FastQCFastQC Report
Thu 26 May 2016
SRR1926598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21299
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA4412.070519742710925No Hit
CCCATGTACTCTGCGTTGATACCAC2711.2723602047044462No Hit
GTACATGGGAAGCAGTGGTATCAAC2631.2347997558570825No Hit
GAGTACATGGGAAGCAGTGGTATCA1460.6854781914643879No Hit
CATGTACTCTGCGTTGATACCACTG1340.6291375181933424No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA1080.5070660594394103No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1060.4976759472275694No Hit
ACGCAGAGTACATGGGAAGCAGTGG940.4413352739565238No Hit
GCGTTGATACCACTGCTTCCCATGT890.4178599934269215No Hit
ACTCTGCGTTGATACCACTGCTTCC890.4178599934269215No Hit
GTATCAACGCAGAGTACATGGGAAG780.3662143762617963No Hit
GGTATCAACGCAGAGTACATGGGAA770.36151932015587585No Hit
CNCATGTACTCTGCGTTGATACCAC670.3145687590966712No Hit
TATCAACGCAGAGTACATGGGAAGC590.2770083102493075No Hit
GNACATGGGAAGCAGTGGTATCAAC590.2770083102493075No Hit
AAACAAAAAAAAAAAAAAAAAAAAA460.21597258087234142No Hit
GTGGTATCAACGCAGAGTACATGGG460.21597258087234142No Hit
GCTTCCCATGTACTCTGCGTTGATA460.21597258087234142No Hit
GTACTCTGCGTTGATACCACTGCTT440.2065824686605005No Hit
GCAGAGTACATGGGAAGCAGTGGTA440.2065824686605005No Hit
CAGTGGTATCAACGCAGAGTACATG400.18780224423681863No Hit
GATACCACTGCTTCCCATGTACTCT370.17371707591905722No Hit
GTATCAACGCAGAGTACTTTTTTTT360.16902201981313678No Hit
AAGCAGTGGTATCAACGCAGAGTAC350.1643269637072163No Hit
GAACAAAAAAAAAAAAAAAAAAAAA350.1643269637072163No Hit
CATGGGAAGCAGTGGTATCAACGCA330.15493685149537537No Hit
ATGGGAAGCAGTGGTATCAACGCAG330.15493685149537537No Hit
GCAGTGGTATCAACGCAGAGTACAT320.15024179538945492No Hit
CCACTGCTTCCCATGTACTCTGCGT320.15024179538945492No Hit
CTGCTTCCCATGTACTCTGCGTTGA320.15024179538945492No Hit
GGGAAGCAGTGGTATCAACGCAGAG310.14554673928353443No Hit
ANAAAAANAAAAAAAAAAAAAAAAA300.14085168317761398No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA300.14085168317761398No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA290.13615662707169351No Hit
TACCACTGCTTCCCATGTACTCTGC290.13615662707169351No Hit
GNGTACATGGGAAGCAGTGGTATCA270.12676651485985257No Hit
NNANNNNAAAANNAAAAAAAAAANA270.12676651485985257No Hit
ATACCACTGCTTCCCATGTACTCTG270.12676651485985257No Hit
ANGCAGAGTACATGGGAAGCAGTGG270.12676651485985257No Hit
ACATGGGAAGCAGTGGTATCAACGC250.11737640264801164No Hit
GGTATCAACGCAGAGTACTTTTTTT240.11268134654209118No Hit
GGAAGCAGTGGTATCAACGCAGAGT230.10798629043617071No Hit
NNAANNANAAAANAAAAAAAAAAAA230.10798629043617071No Hit
CCATGTACTCTGCGTTGATACCACT230.10798629043617071No Hit
CNTGTACTCTGCGTTGATACCACTG230.10798629043617071No Hit
GNTATCAACGCAGAGTACATGGGAA220.10329123433025025No Hit
ANTCTGCGTTGATACCACTGCTTCC220.10329123433025025No Hit
NNAANANAAAAANAAAAAAAAAAAA220.10329123433025025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAAA500.00821948613.2020633
TACTCTG1352.331973E-59.0919427
GTACTCT1301.239128E-48.8528156
ATGTACT1000.0090682498.6235694
TGTACTC1258.44839E-48.3699355
TGCGTTG1202.5109068E-48.313621512
TGGGAAG1559.029928E-58.0999376
CTGCGTT1401.2236035E-47.908192611
CGTTGAT1606.889528E-67.44765914
ACTCTGC1458.343989E-47.4272918
CTCTGCG1502.7242402E-47.380989
ATGGGAA1753.6921594E-47.17422965
TCTGCGT1553.975735E-47.14288410
GGGAAGC1600.00148354917.0320497
ATACCAC1454.8579165E-47.02789719
GTTGATA1655.907502E-56.929653615
GCGTTGA1450.0019046696.880238513
CAGTGGT1856.5540706E-56.86333313
TGATACC1657.2383045E-56.825186317
CATGGGA1750.0021794316.7756614
Produced by FastQC (version 0.11.5)