FastQCFastQC Report
Thu 26 May 2016
SRR1926598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21299
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC8343.9156767923376683No Hit
GTACATGGGAAGCAGTGGTATCAAC7123.342879947415372No Hit
GTATCAACGCAGAGTACTTTTTTTT4382.056434574393164No Hit
GAGTACATGGGAAGCAGTGGTATCA3891.8263768252030612No Hit
CATGTACTCTGCGTTGATACCACTG3321.5587586271655947No Hit
GGTATCAACGCAGAGTACTTTTTTT3061.4366871684116627No Hit
ACGCAGAGTACATGGGAAGCAGTGG2791.30992065355181No Hit
TATCAACGCAGAGTACTTTTTTTTT2311.0845579604676274No Hit
GCGTTGATACCACTGCTTCCCATGT2080.976571670031457No Hit
ACTCTGCGTTGATACCACTGCTTCC2070.9718766139255365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2060.967181557819616No Hit
GTATCAACGCAGAGTACATGGGAAG1820.8545002112775247No Hit
GGTATCAACGCAGAGTACATGGGAA1730.8122447063242406No Hit
TATCAACGCAGAGTACATGGGAAGC1570.7371238086295131No Hit
GCTTCCCATGTACTCTGCGTTGATA1160.5446265082867741No Hit
CATGGGAAGCAGTGGTATCAACGCA1120.5258462838630922No Hit
GTGGTATCAACGCAGAGTACATGGG1070.5023710033334898No Hit
GCAGAGTACATGGGAAGCAGTGGTA1060.4976759472275694No Hit
CAGTGGTATCAACGCAGAGTACATG1050.49298089112164895No Hit
CTGCTTCCCATGTACTCTGCGTTGA1040.4882858350157285No Hit
ATACCACTGCTTCCCATGTACTCTG980.4601154983802057No Hit
ACATGGGAAGCAGTGGTATCAACGC970.4554204422742852No Hit
GCAGTGGTATCAACGCAGAGTACAT950.44603033006244425No Hit
GATACCACTGCTTCCCATGTACTCT910.42725010563876237No Hit
GTACTCTGCGTTGATACCACTGCTT900.42255504953284195No Hit
GAGTACTTTTTTTTTTTTTTTTTTT820.3849946006854782No Hit
GGGAAGCAGTGGTATCAACGCAGAG820.3849946006854782No Hit
CTTCCCATGTACTCTGCGTTGATAC810.3802995445795577No Hit
CCACTGCTTCCCATGTACTCTGCGT790.3709094323677168No Hit
GCAGAGTACTTTTTTTTTTTTTTTT780.3662143762617963No Hit
GTACTTTTTTTTTTTTTTTTTTTTT700.3286539274144326No Hit
AAGCAGTGGTATCAACGCAGAGTAC670.3145687590966712No Hit
TACCACTGCTTCCCATGTACTCTGC620.29109347856706885No Hit
GTTGATACCACTGCTTCCCATGTAC600.28170336635522797No Hit
ATCAACGCAGAGTACTTTTTTTTTT570.2676181980374665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA550.2582280858256256No Hit
ATGGGAAGCAGTGGTATCAACGCAG550.2582280858256256No Hit
CCATGTACTCTGCGTTGATACCACT530.2488379736137847No Hit
ATCAACGCAGAGTACATGGGAAGCA470.2206676369782619No Hit
GTGGTATCAACGCAGAGTACTTTTT430.20188741255458004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA340.15963190760129584No Hit
AAAAAGTACTCTGCGTTGATACCAC330.15493685149537537No Hit
CTCTGCGTTGATACCACTGCTTCCC320.15024179538945492No Hit
GTACATGGTAAGCAGTGGTATCAAC320.15024179538945492No Hit
AAAGTACTCTGCGTTGATACCACTG260.12207145875393212No Hit
GTACATGGAAAGCAGTGGTATCAAC250.11737640264801164No Hit
GGAAGCAGTGGTATCAACGCAGAGT240.11268134654209118No Hit
TGGGAAGCAGTGGTATCAACGCAGA230.10798629043617071No Hit
AACGCAGAGTACTTTTTTTTTTTTT230.10798629043617071No Hit
TCAACGCAGAGTACATGGGAAGCAG230.10798629043617071No Hit
CTGCGTTGATACCACTGCTTCCCAT220.10329123433025025No Hit
CAACGCAGAGTACATGGGAAGCAGT220.10329123433025025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA2501.5825208E-108.33101218
TGATACC2459.731593E-108.11462217
ATACCAC2603.5288394E-108.01058919
CCATGTA2404.944013E-97.96417242
CTGCGTT2801.6043487E-97.43840311
TTGATAC2551.832268E-87.42514416
CCCATGT2602.195884E-87.3515441
ACTCTGC2852.1864253E-97.3251418
TACTCTG2902.9613147E-97.2158647
TGTACTC2801.08993845E-87.16775565
GTACTCT2953.794412E-97.12725936
TGCGTTG2801.31385605E-87.10029412
CTCTGCG3006.164555E-96.95888339
TCTGCGT3006.4701453E-96.942509710
ATGTACT2902.1451342E-86.9205924
CATGTAC2952.9796865E-86.8032933
GTTGATA2801.0277108E-76.76218515
CGTTGAT2953.5866833E-86.73926214
GCGTTGA3109.2486516E-86.41316913
AGTGGTA2050.0010733446.003500514
Produced by FastQC (version 0.11.5)