FastQCFastQC Report
Thu 26 May 2016
SRR1926597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912133
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10880.11928085049000531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAA258.2122756E-426.77581
TAGGACC3100.016.6153724
TCTTAGA603.6881206E-416.0017662
GTGTAGG952.8830618E-715.50178051
TGTAGGA6750.015.4429752
GTCCTAC8350.014.5904071
CTGTAGG6950.014.4473741
TCCTACA9800.013.9957142
TGTAGAA3050.013.940652
TATAGGA1703.6379788E-1113.7157992
CAGGACT1600.013.413454
GTCTATA700.001424654413.38791
GCCCTGA904.6088095E-513.38791
GTCCAAG600.00797116513.38791
GTAGGAC7950.013.3794763
ACAGGAC755.4232783E-513.1587833
CTTAGAC500.00893706113.1587833
CTAGGCC600.00200644512.79326253
ACAGATC1301.2551027E-1012.7620898
TAGACTA902.6149155E-612.7508645
Produced by FastQC (version 0.11.5)