FastQCFastQC Report
Thu 26 May 2016
SRR1926597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912133
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22370.24524932219314508No Hit
GTCCTACAGTGGACATTTCTAAATT21630.23713647022967047No Hit
GTATCAACGCAGAGTACTTTTTTTT16720.1833066011206699No Hit
GTCCTACAGTGTGCATTTCTCATTT16320.17892127573500793No Hit
CTTTAGGACGTGAAATATGGCGAGG15300.16773869600156993No Hit
CTGTAGGACCTGGAATATGGCGAGA14910.16346300375054953No Hit
GTCCTAAAGTGTGTATTTCTCATTT14420.15809098015311362No Hit
CCCATGTACTCTGCGTTGATACCAC12270.13451985620518062No Hit
GTACATGGGAAGCAGTGGTATCAAC12190.13364279112804822No Hit
GGTATCAACGCAGAGTACTTTTTTT10410.11412809316185249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC1050.016.25242413
TGCCGGT1151.8189894E-1214.84161110
CCGGTTT1151.8189894E-1214.839169512
CCTGCGT400.005262003714.2560638
TAGGACC5100.013.8358324
GCCGGTT1257.2759576E-1213.65203611
CCAACGT802.059638E-613.02650719
TGTAGGA12100.013.0072682
CCAACGA905.58035E-712.63176519
TCCAACG1750.012.45131218
TAGGACA4000.012.3961564
GGACTGC1001.3725366E-712.39547356
GTCTTAC853.7866503E-612.3373431
GTAGGAC12700.012.2411853
CTGTAGG12550.012.2301021
AGGACCT7950.012.114295
GTCTTAG959.950836E-712.04219151
TTAGGAC6400.011.7729883
GGACCTG7900.011.7075066
TATAGGA2050.011.6325162
Produced by FastQC (version 0.11.5)