FastQCFastQC Report
Thu 26 May 2016
SRR1926596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707665
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA450.001220934117.7426951
TGCGCCA350.002981913515.52600512
TAGGACC1400.015.2642574
TGTAGGA4000.014.9879182
GTCCTAT550.00465975514.5167511
CTGTAGG3900.014.3306391
GTCTATA658.515167E-414.3306391
GTTCAAG658.515167E-414.3306391
GTATAGT852.8468028E-514.08978751
GTCCTAC5450.013.6732681
GCGCCAC400.007233588513.58639713
GTAGGAC4300.013.1911133
AGGACGT4500.013.1657635
TATGAAC755.6931854E-513.08964353
TTTGGGC500.00924972113.08964353
TCCTACA6000.012.9441112
CAGGACT756.910158E-512.8219784
CCAACGA607.006058E-512.80418319
CAGGACC1101.0738586E-712.6277064
GACGTGG2100.012.5891927
Produced by FastQC (version 0.11.5)