##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926596_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 707665 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.248431107939492 27.0 7.0 31.0 2.0 31.0 2 16.34992404598221 16.0 2.0 30.0 2.0 31.0 3 19.900859870136294 23.0 15.0 31.0 2.0 31.0 4 21.990292016702817 28.0 2.0 35.0 2.0 37.0 5 22.82130810482361 30.0 2.0 35.0 2.0 37.0 6 23.508285700154733 31.0 2.0 35.0 2.0 37.0 7 23.173692354433243 30.0 7.0 35.0 2.0 37.0 8 23.47577596744222 31.0 8.0 35.0 2.0 37.0 9 26.088050136717232 31.0 17.0 37.0 2.0 39.0 10 26.816017465891345 32.0 23.0 37.0 2.0 39.0 11 23.963765340945223 30.0 7.0 37.0 2.0 39.0 12 25.09644393886938 31.0 10.0 37.0 2.0 39.0 13 21.7566843068401 27.0 2.0 35.0 2.0 39.0 14 25.054121653607286 31.0 17.0 38.0 2.0 40.0 15 26.158949502942775 31.0 17.0 38.0 2.0 40.0 16 26.52310203274148 31.0 17.0 38.0 2.0 40.0 17 25.8273632297768 31.0 10.0 38.0 2.0 40.0 18 25.8761885920598 31.0 11.0 38.0 2.0 40.0 19 26.276156090805678 31.0 16.0 39.0 2.0 40.0 20 26.552885899401552 31.0 16.0 39.0 2.0 40.0 21 24.386603830908694 30.0 2.0 37.0 2.0 40.0 22 25.31600262836229 31.0 2.0 38.0 2.0 40.0 23 25.305337977715446 31.0 2.0 38.0 2.0 40.0 24 24.389562858132027 31.0 2.0 38.0 2.0 40.0 25 24.18064479661987 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.700903318140128 1101 2 10.407663468554162 1101 3 6.961981425519763 1101 4 8.609623145468106 1101 5 8.109030887438273 1101 6 7.722040337892061 1101 7 7.226727023955522 1101 8 5.7623177554224405 1101 9 5.817168400433058 1101 10 5.321943489385411 1101 11 7.99606793508951 1101 12 5.754170545140333 1101 13 10.266407367139472 1101 14 7.675359754325061 1101 15 6.795399876835759 1101 16 6.459223600028025 1101 17 2.507713686226591 1101 18 4.618244033304418 1101 19 5.444609265970001 1101 20 3.42532455804907 1101 21 0.5425618914448904 1101 22 4.222314122679528 1101 23 5.57222606532893 1101 24 6.843052859172776 1101 25 7.303300269634441 1102 1 5.703400946850614 1102 2 10.442255140274398 1102 3 6.991513698381329 1102 4 7.923901936551022 1102 5 8.049037262708445 1102 6 7.1213194917952265 1102 7 6.871258415682181 1102 8 7.075499015238023 1102 9 5.857563252531975 1102 10 5.470018209625078 1102 11 8.119316679472277 1102 12 5.6058936821879115 1102 13 10.038566614131952 1102 14 7.511208209488249 1102 15 5.173161198123463 1102 16 5.751510300592468 1102 17 6.5867139091018885 1102 18 6.288010869459974 1102 19 5.025116488684624 1102 20 5.386147745373169 1102 21 4.366343981600544 1102 22 5.3738615510614025 1102 23 5.905312551311113 1102 24 7.435298612880022 1102 25 7.030066815533793 1103 1 4.745529451144893 1103 2 8.794738530324633 1103 3 5.875819321457005 1103 4 7.243966215706209 1103 5 6.8700306187285385 1103 6 6.151113420385947 1103 7 6.419361100282195 1103 8 5.840853090752525 1103 9 4.767394182149001 1103 10 4.223829045254096 1103 11 4.843145880574205 1103 12 3.8985745010719377 1103 13 7.3853769636161 1103 14 5.863476885553716 1103 15 5.060228394147877 1103 16 4.671077631657123 1103 17 2.242882547356089 1103 18 1.1006546873176966 1103 19 3.1217944987316173 1103 20 3.628215481558552 1103 21 5.8369727289672575 1103 22 1.7276345754226625 1103 23 3.6256455605478664 1103 24 4.9337240436583905 1103 25 0.82315005433896 1104 1 5.252162144620456 1104 2 9.646720153116593 1104 3 6.374085896589683 1104 4 6.7461419081520155 1104 5 6.852708251870883 1104 6 6.5972769141677965 1104 7 6.189622236183759 1104 8 6.211955195154079 1104 9 5.01987088058852 1104 10 4.020160204655252 1104 11 7.091627648588357 1104 12 4.063331715974375 1104 13 8.535147248515631 1104 14 6.139410928275446 1104 15 5.361098407085855 1104 16 4.930940858758227 1104 17 5.385493378291056 1104 18 2.921718505869169 1104 19 3.741601485239155 1104 20 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5 1.1701121275775925 2204 6 4.113948057638165 2204 7 5.045201409748579 2204 8 4.982357797554506 2204 9 3.4084559351237402 2204 10 3.14634085401563 2204 11 4.476295930348051 2204 12 4.658389970382519 2204 13 2.565167445787708 2204 14 3.8504976034996936 2204 15 3.9915832449265523 2204 16 3.939881121839317 2204 17 -1.1983382146848385 2204 18 2.4661557329025214 2204 19 3.461497993590548 2204 20 3.6678935884153177 2204 21 4.992744824880457 2204 22 4.547793317246722 2204 23 2.887652415308448 2204 24 4.680405898586002 2204 25 4.842562522520442 2205 1 5.804079077527806 2205 2 10.222598747365797 2205 3 6.8141817432350535 2205 4 7.621541269174379 2205 5 7.585935088931585 2205 6 7.0765101410048565 2205 7 7.165660574841908 2205 8 7.025054236687623 2205 9 5.787442765572546 2205 10 5.274153814878893 2205 11 7.703448734850337 2205 12 6.7438428980830025 2205 13 9.776361306023617 2205 14 7.222478020927202 2205 15 5.976913103571476 2205 16 5.59228271179337 2205 17 6.38868826646997 2205 18 6.073475694367573 2205 19 4.8110903031305305 2205 20 5.31521809555797 2205 21 -1.0768841083574863 2205 22 3.726792286720947 2205 23 4.879589003105888 2205 24 6.773006646040034 2205 25 7.195193462571737 2206 1 4.2143646563437365 2206 2 8.843753152066526 2206 3 6.011292938748802 2206 4 2.114849877498962 2206 5 5.677002434791266 2206 6 6.027513787695405 2206 7 6.222914112926116 2206 8 6.300555004999893 2206 9 4.816359358386904 2206 10 3.9428231824300504 2206 11 3.1401014502570455 2206 12 4.374233185821531 2206 13 7.705324692654738 2206 14 0.2690924820784062 2206 15 2.505266184640213 2206 16 3.4278551975255986 2206 17 4.6218046353690845 2206 18 -0.8859173265270464 2206 19 1.2679977903894546 2206 20 2.703506856849927 2206 21 3.363955846859465 2206 22 -2.100725952937868 2206 23 -2.6756612134861832 2206 24 1.2912221011564498 2206 25 2.713564690208141 2207 1 1.1806684504567464 2207 2 -13.736666428353054 2207 3 -6.249918767895402 2207 4 -4.851338340103968 2207 5 0.8984872286285253 2207 6 1.5359367399340407 2207 7 2.820464142165715 2207 8 2.930619721564206 2207 9 0.5426515697235779 2207 10 0.4195825678393952 2207 11 -1.7112545951167846 2207 12 -2.347268881678918 2207 13 0.3800421426349203 2207 14 -5.257828993104994 2207 15 -1.4449567712415963 2207 16 -0.43521992909035134 2207 17 0.8388485274331892 2207 18 0.8331727094587045 2207 19 0.18098061283064482 2207 20 -0.2488404455377946 2207 21 -3.9288396536967447 2207 22 -4.580743465291675 2207 23 -1.6903589023956727 2207 24 0.06924987595059306 2207 25 0.6162084400628842 2208 1 4.68108921487406 2208 2 6.197324466942849 2208 3 6.022137935936744 2208 4 8.03488659343856 2208 5 3.2712470874577697 2208 6 5.82342433474404 2208 7 6.383441412300151 2208 8 6.375121140264056 2208 9 5.0109249512833 2208 10 4.03155926509633 2208 11 6.286109800362514 2208 12 5.851204957937462 2208 13 8.247746694864297 2208 14 3.027952236385808 2208 15 4.493721415128828 2208 16 4.935902874010303 2208 17 5.9597563481311155 2208 18 0.44625345329168553 2208 19 2.917585767746779 2208 20 4.112530559500335 2208 21 6.856517744580696 2208 22 5.960673615290229 2208 23 1.744317728495794 2208 24 3.2991123730831404 2208 25 4.46431309877142 2209 1 3.490506694430451 2209 2 5.347155868377875 2209 3 5.599303419466743 2209 4 0.628405056603178 2209 5 5.3996815438911625 2209 6 6.21176196942892 2209 7 6.502041058440319 2209 8 5.279811754583552 2209 9 5.231276470705524 2209 10 4.242347538115347 2209 11 -0.15569030571911924 2209 12 3.711118057492655 2209 13 2.9324629264987543 2209 14 4.5911146901022875 2209 15 5.1345365138267205 2209 16 4.479445313658886 2209 17 2.5033038756277577 2209 18 4.407533657619663 2209 19 4.889587339891829 2209 20 4.94865288154331 2209 21 6.865822146714031 2209 22 5.7550749472888505 2209 23 0.9853443544766378 2209 24 -2.796832993604429 2209 25 0.7386377823390688 2210 1 -2.303439849169287 2210 2 -13.736666428353054 2210 3 -7.6586911596813 2210 4 -2.2078169201458877 2210 5 -0.5536112593869618 2210 6 -1.263899023471577 2210 7 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19 -8.800512582505405 2211 20 -6.910631674657914 2211 21 -4.672350923005482 2211 22 -6.322327098653684 2211 23 -8.099932464064345 2211 24 -21.8571850472507 2211 25 -21.65784168119823 2212 1 -0.6817477474332314 2212 2 -13.736666428353054 2212 3 -7.550278942931284 2212 4 -19.381653619004535 2212 5 -20.122531364742535 2212 6 -20.810870050688433 2212 7 -9.716884791705972 2212 8 -9.228183864586585 2212 9 -4.8555003919219 2212 10 -5.098912759215718 2212 11 -5.263986596752552 2212 12 -2.6372123368954057 2212 13 -5.967509636626843 2212 14 -3.479955884643541 2212 15 -3.4562088697113076 2212 16 -3.50143352797593 2212 17 -3.7529829855862538 2212 18 -2.7125438609984833 2212 19 -3.24161382832947 2212 20 -3.5286580326639267 2212 21 -1.5194611068911215 2212 22 -2.7693487838690842 2212 23 -2.8096598668716695 2212 24 -1.7904582663670183 2212 25 -2.268301554958377 2213 1 -18.65199121001213 2213 2 -13.736666428353054 2213 3 -8.560532337424299 2213 4 -5.712088401613231 2213 5 -20.122531364742535 2213 6 -10.842754108659447 2213 7 -20.500473610461608 2213 8 -10.626859609371241 2213 9 -8.502737969406077 2213 10 -7.237711258973956 2213 11 -6.416190566904536 2213 12 -5.360829818806689 2213 13 -19.007185019854976 2213 14 -12.193324328164493 2213 15 -6.995203394090236 2213 16 -6.248844913957122 2213 17 -4.77414144753352 2213 18 -4.736591290244487 2213 19 -5.598810763375621 2213 20 -5.786145519758971 2213 21 -3.9107375756600433 2213 22 -4.805479170734564 2213 23 -4.541895462329126 2213 24 -3.5422092018400697 2213 25 -4.222092888927701 2214 1 -18.65199121001213 2214 2 -13.736666428353054 2214 3 -12.287491264599716 2214 4 -19.381653619004535 2214 5 -20.122531364742535 2214 6 -20.810870050688433 2214 7 -16.287818008801857 2214 8 -14.988490896079202 2214 9 -13.947504140953773 2214 10 -13.868386585211251 2214 11 -14.520379032837544 2214 12 -12.558793216084538 2214 13 -19.007185019854976 2214 14 -16.73051337541127 2214 15 -23.56621788684386 2214 16 -18.208338369165894 2214 17 -23.267861254296804 2214 18 -17.749648800217024 2214 19 -15.093222133071134 2214 20 -13.766748682907687 2214 21 -11.319503386392299 2214 22 -12.404221328010006 2214 23 -12.257642049616395 2214 24 -11.188097910321238 2214 25 -10.94103670194512 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129062.0 3 6.0 4 592.0 5 2346.0 6 3301.0 7 3258.0 8 2714.0 9 2616.0 10 2422.0 11 2278.0 12 2193.0 13 2241.0 14 3330.0 15 7054.0 16 10460.0 17 11227.0 18 11627.0 19 13352.0 20 15764.0 21 18444.0 22 19040.0 23 20795.0 24 21940.0 25 22443.0 26 18758.0 27 14976.0 28 16151.0 29 18497.0 30 18108.0 31 20913.0 32 26408.0 33 33619.0 34 40395.0 35 45477.0 36 45097.0 37 46513.0 38 34248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54234645147457 19.280224028484362 12.375332176805774 25.802097343235285 2 14.118325901394346 20.6730971177931 41.14390695227125 24.0646700285413 3 18.35363581816196 25.086449531578953 29.151036421774958 27.40887822848413 4 11.314986707078889 17.99704746911927 38.70020053694256 31.987765286859275 5 12.23168554262404 37.62853679834222 37.251723598639366 12.888054060394374 6 31.30275817725921 37.923904258009486 18.359308497010748 12.414029067720548 7 27.84857022773618 31.484721595242917 22.833704332124345 17.83300384489656 8 24.015940999977094 38.73601917806128 20.629547345838773 16.618492476122857 9 26.026794556206323 15.823784059078177 20.598279715926775 37.55114166878873 10 13.963178822375946 30.091078232101854 35.15771699524971 20.78802595027249 11 32.68866646609699 22.935142651441332 25.138116171753506 19.238074710708176 12 23.188661870072696 25.2414144212268 32.14865947427134 19.421264234429163 13 27.812446981315585 22.06853919561102 25.447120738977574 24.67189308409582 14 19.989033025808276 23.020741074619924 28.187276874469774 28.802949025102027 15 21.84868447853439 32.17498967020305 23.816729033421986 22.159596817840576 16 21.21276038636099 28.894448970091936 28.438554637495635 21.454236006051435 17 20.842011565805244 28.960328357166958 29.25121883108572 20.946441245942076 18 20.74052933060646 27.97916983383482 31.070708403489096 20.20959243206962 19 22.22946928115069 27.60431822775483 27.944197327698785 22.222015163395696 20 22.728348470827317 28.14425991613323 28.76369495623141 20.363696656808045 21 21.48978626540929 26.75584592189671 27.269636680757976 24.484731131936027 22 21.913949782505707 28.81677361930603 28.405616090270897 20.863660507917366 23 21.2412766721981 28.502735301732002 28.732480841570364 21.523507184499532 24 21.779410714230256 27.946195917175753 29.011780125037657 21.262613243556334 25 21.91365316680979 27.923581765219346 28.685981549048094 21.476783518922765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 888.0 1 888.0 2 1735.5 3 2583.0 4 2583.0 5 2583.0 6 3609.5 7 4636.0 8 4636.0 9 4636.0 10 5575.0 11 6514.0 12 6514.0 13 6514.0 14 8156.5 15 9799.0 16 9799.0 17 9799.0 18 13874.0 19 17949.0 20 17949.0 21 17949.0 22 24250.5 23 30552.0 24 30552.0 25 30552.0 26 39265.0 27 47978.0 28 47978.0 29 47978.0 30 57189.5 31 66401.0 32 66401.0 33 66401.0 34 72549.5 35 78698.0 36 78698.0 37 78698.0 38 82111.0 39 85524.0 40 85524.0 41 85524.0 42 86135.0 43 86746.0 44 86746.0 45 86746.0 46 84686.0 47 82626.0 48 82626.0 49 82626.0 50 75808.0 51 68990.0 52 68990.0 53 68990.0 54 59151.5 55 49313.0 56 49313.0 57 49313.0 58 41103.5 59 32894.0 60 32894.0 61 32894.0 62 26003.5 63 19113.0 64 19113.0 65 19113.0 66 14473.5 67 9834.0 68 9834.0 69 9834.0 70 7017.0 71 4200.0 72 4200.0 73 4200.0 74 2918.0 75 1636.0 76 1636.0 77 1636.0 78 1103.0 79 570.0 80 570.0 81 570.0 82 364.5 83 159.0 84 159.0 85 159.0 86 103.5 87 48.0 88 48.0 89 48.0 90 29.0 91 10.0 92 10.0 93 10.0 94 7.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.004599634007615 2 36.081761850593146 3 5.561388510100118 4 13.468095779782807 5 13.259522514183972 6 14.507429362763455 7 9.221171034317086 8 7.454091978549172 9 0.11375438943567931 10 1.4163481308246133 11 5.591346187814856 12 5.6283693555566545 13 15.877003949608925 14 1.558364480368536 15 2.8732521744045556 16 2.857990715946104 17 2.843294496689818 18 1.4706110942324406 19 1.4221418326468034 20 0.2857284166943398 21 4.398267541845365 22 1.5678322370047977 23 0.11248260123080836 24 9.001292984674953 25 0.11559141684271512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 707665.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.8241997432602 #Duplication Level Percentage of deduplicated Percentage of total 1 97.29113804177791 91.2826316888087 2 2.017135765658189 3.7851229797277606 3 0.3232635008559261 0.9098981782203592 4 0.12246466457708467 0.4596059660308701 5 0.06677259807653872 0.31324427896548 6 0.04122716886885994 0.232086367608062 7 0.02434928400802548 0.15991864604620476 8 0.018268434774601376 0.13712170186311362 9 0.012062266663840266 0.10185592651421563 >10 0.0720877420029133 1.2192851001876255 >50 0.006769204931000362 0.45699000593767103 >100 0.004153650028119756 0.7714871806406237 >500 3.0767777703060155E-4 0.1707519794493507 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4130980054121654E-4 0.0 0.0 0.0 6 0.0 1.4130980054121654E-4 0.0 0.0 0.0 7 0.0 1.4130980054121654E-4 0.0 0.0 0.0 8 0.0 1.4130980054121654E-4 0.0 0.0 0.0 9 0.0 1.4130980054121654E-4 0.0 0.0 0.0 10 0.0 1.4130980054121654E-4 0.0 0.0 0.0 11 0.0 1.4130980054121654E-4 0.0 1.4130980054121654E-4 0.0 12 0.0 1.4130980054121654E-4 0.0 1.4130980054121654E-4 1.4130980054121654E-4 13 0.0 1.4130980054121654E-4 0.0 1.4130980054121654E-4 1.4130980054121654E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAA 45 0.0012209341 17.742695 1 TGCGCCA 35 0.0029819135 15.526005 12 TAGGACC 140 0.0 15.264257 4 TGTAGGA 400 0.0 14.987918 2 GTCCTAT 55 0.004659755 14.516751 1 CTGTAGG 390 0.0 14.330639 1 GTCTATA 65 8.515167E-4 14.330639 1 GTTCAAG 65 8.515167E-4 14.330639 1 GTATAGT 85 2.8468028E-5 14.0897875 1 GTCCTAC 545 0.0 13.673268 1 GCGCCAC 40 0.0072335885 13.586397 13 GTAGGAC 430 0.0 13.191113 3 AGGACGT 450 0.0 13.165763 5 TATGAAC 75 5.6931854E-5 13.0896435 3 TTTGGGC 50 0.009249721 13.0896435 3 TCCTACA 600 0.0 12.944111 2 CAGGACT 75 6.910158E-5 12.821978 4 CCAACGA 60 7.006058E-5 12.804183 19 CAGGACC 110 1.0738586E-7 12.627706 4 GACGTGG 210 0.0 12.589192 7 >>END_MODULE