FastQCFastQC Report
Thu 26 May 2016
SRR1926596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707665
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14460.20433397158259914No Hit
GTCCTACAGTGGACATTTCTAAATT13670.193170497339843No Hit
CTGTAGGACGTGGAATATGGCAAGA13550.1914747797333484No Hit
CTTTAGGACGTGAAATATGGCGAGG11180.1579843570050801No Hit
GTCCTAAAGTGTGTATTTCTCATTT10800.15261458458451385No Hit
GTACATGGGAAGCAGTGGTATCAAC9700.13707050652498004No Hit
GGTATCAACGCAGAGTACTTTTTTT9410.13297252230928475No Hit
CCCATGTACTCTGCGTTGATACCAC9220.13028763609900165No Hit
GTCCTACAGTGTGCATTTCTCATTT8180.11559141684271512No Hit
CTGTAGGACCTGGAATATGGCGAGA7910.11177605222810229No Hit
TATCAACGCAGAGTACTTTTTTTTT7860.1110695032253962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT350.002200147116.25056812
TTTAGGC500.001454315513.3524793
TTAGACT751.4183299E-512.7157424
TAGGACC3700.012.6298264
TGTAGGA9100.012.57869152
CTGTAGG8450.012.5222951
TCGTCAA701.0485489E-412.2642252
ACTGTTC1551.8189894E-1212.2619518
GGCTTAC701.05483574E-412.2563781
AAAGGCG550.002978057612.13775355
GTATTAG550.00298759312.1325761
GTAGGAC8650.012.1285933
TTAGGAC4400.011.9218563
TAAAGGC802.7501119E-511.9210084
GGACGTG7550.011.8736396
CTCCTAG657.785852E-411.7326011
GGCGAGG2750.011.71348219
CGAAATC658.1604905E-411.66707413
ACGAAAT658.1604905E-411.66707412
GACGTGG4400.011.6644417
Produced by FastQC (version 0.11.5)