FastQCFastQC Report
Thu 26 May 2016
SRR1926595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744240
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.003594330320.7757975
GTAGGAC1950.017.3494433
TAGGACC1300.015.6422544
TGTAGGA2700.015.6404232
GTCCTAC2300.015.6059071
TCCTACA2900.014.5617732
GTCTAGG550.0046908614.502461
TAGGACG1353.6379788E-1214.2701244
GTCCTAG751.5695806E-414.18018151
CTAACAC702.9634266E-514.0314633
GTGTTAA600.00834794513.29392051
CTGTAGG2750.013.0522131
TTATACA1357.185008E-1012.6845554
GATTAAG854.526862E-412.5119251
CTACAGT3000.012.486364
CCTACAG2450.012.4724133
ATTATAC704.652196E-412.4724113
CTTATAC1151.4552643E-712.3368433
AGGACGT1609.094947E-1212.3356295
GTATAAA1256.8291592E-611.698651
Produced by FastQC (version 0.11.5)