FastQCFastQC Report
Thu 26 May 2016
SRR1926595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744240
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12110.1627163280662152No Hit
GGTATCAACGCAGAGTACTTTTTTT7480.10050521337203053No Hit
CACACACACACACACACACACACAC7460.10023648285499302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGT759.257692E-713.9850944
CCGTGCA500.00149982313.2981619
TAGGACC1850.012.3701094
GTCTTAT701.0563367E-412.2546891
GCTATAC1103.603236E-812.1374663
CTAGGAC1501.2732926E-1112.079673
CTTCTAG1209.498763E-911.9142811
GTAGGAC3450.011.8861743
GACAGTT802.8696426E-511.8717597
CCTGTAC1406.348273E-1011.5801343
TGTAGGA3950.011.3480922
CTTAGGC600.00570514511.1260113
TGGACTA600.005710893311.1245075
TAAGGTA600.005710893311.1245075
TGGGCCG600.005710893311.1245075
AGGACGT2250.011.0185595
CCTAGAC700.001442788610.8989513
TAGGACA1751.8189894E-1110.8974764
GCCCTAG700.001449315610.8930581
TAGGACG2400.010.7272034
Produced by FastQC (version 0.11.5)