FastQCFastQC Report
Thu 26 May 2016
SRR1926594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824
Sequences flagged as poor quality0
Sequence length25
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA873.080736543909348No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA431.5226628895184136No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA180.6373937677053824No Hit
ACACAAAAAAAAAAAAAAAAAAAAA130.46033994334277617No Hit
GAACAAAAAAAAAAAAAAAAAAAAA120.424929178470255No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA90.3186968838526912No Hit
AAACAAAAAAAAAAAAAAAAAAAAA80.28328611898017No Hit
AACAAAAAAAAAAAAAAAAAAAAAA70.24787535410764872No Hit
NNAAAANAAAAAAAAAAAAAAAAAA70.24787535410764872No Hit
NNANNNAAAAAANAAAAAAAAAANA60.2124645892351275No Hit
NNAANANAAAAANAAAAAAAAAAAA60.2124645892351275No Hit
NNANNNNAAAANNAAAAAAAAAANA60.2124645892351275No Hit
ANNAAAAAAANAAAAAAAAANAANA60.2124645892351275No Hit
ANAANANAAAAANAACAAAAAAAAA60.2124645892351275No Hit
GGTATCAACGCAGAGTACTTTTTTT60.2124645892351275No Hit
GNACAAAAAAAAAAAAAAAAAAAAA50.17705382436260625No Hit
ACACAAAAAACAAAAAAAAAAAAAA50.17705382436260625No Hit
ACANAAAAAAAAAAAAAAAAAAAAA50.17705382436260625No Hit
GAAAACAAAAAAAAAAAAAAAAAAA50.17705382436260625No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA40.141643059490085No Hit
NNAANNANAAAANAAAAAAAAAAAA40.141643059490085No Hit
CCCATGTACTCTGCGTTGATACCAC40.141643059490085No Hit
GTACATGGGAAGCAGTGGTATCAAC40.141643059490085No Hit
ANACAAAAAAAAAAAAAAAAAAAAA40.141643059490085No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA40.141643059490085No Hit
ACTCTGCGTTGATACCACTGCTTCC30.10623229461756376No Hit
ANAAAAANAAAAAAAAAAAAAAAAA30.10623229461756376No Hit
NNANNNAAAAAANANANAAAAAAAA30.10623229461756376No Hit
GGTATCAACGCAGAGTACATGGGGG30.10623229461756376No Hit
ACAAAAANAAAAAAAAAAAAAAANA30.10623229461756376No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)