FastQCFastQC Report
Thu 26 May 2016
SRR1926594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1123.9660056657223794No Hit
GGTATCAACGCAGAGTACTTTTTTT923.2577903682719547No Hit
TATCAACGCAGAGTACTTTTTTTTT652.301699716713881No Hit
ACGCAGAGTACTTTTTTTTTTTTTT451.593484419263456No Hit
GCAGAGTACTTTTTTTTTTTTTTTT291.0269121813031163No Hit
ATCAACGCAGAGTACTTTTTTTTTT210.7436260623229463No Hit
GTACTTTTTTTTTTTTTTTTTTTTT200.708215297450425No Hit
GTGGTATCAACGCAGAGTACTTTTT200.708215297450425No Hit
GAGTACTTTTTTTTTTTTTTTTTTT180.6373937677053824No Hit
CCCATGTACTCTGCGTTGATACCAC140.49575070821529743No Hit
GTACATGGGAAGCAGTGGTATCAAC130.46033994334277617No Hit
GAGTACATGGGAAGCAGTGGTATCA110.3895184135977337No Hit
CAACGCAGAGTACTTTTTTTTTTTT90.3186968838526912No Hit
CATGTACTCTGCGTTGATACCACTG70.24787535410764872No Hit
TCAACGCAGAGTACTTTTTTTTTTT60.2124645892351275No Hit
AACGCAGAGTACTTTTTTTTTTTTT60.2124645892351275No Hit
CAGTGGTATCAACGCAGAGTACATG60.2124645892351275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60.2124645892351275No Hit
GTATCAACGCAGAGTACATGGGAAG60.2124645892351275No Hit
TATCAACGCAGAGTACATGGGAAGC50.17705382436260625No Hit
GTTATCAACGCAGAGTACTTTTTTT50.17705382436260625No Hit
CCACTGCTTCCCATGTACTCTGCGT40.141643059490085No Hit
ACGCAGAGTACATGGGAAGCAGTGG40.141643059490085No Hit
GGGATCAACGCAGAGTACTTTTTTT40.141643059490085No Hit
GTATCAACGCAGATTACTTTTTTTT40.141643059490085No Hit
GCGTTGATACCACTGCTTCCCATGT40.141643059490085No Hit
GTATCAACGCAGAGTACATGGGGGT30.10623229461756376No Hit
TACCACTGCTTCCCATGTACTCTGC30.10623229461756376No Hit
CGCAGAGTACTTTTTTTTTTTTTTT30.10623229461756376No Hit
CATCTAAGTACCCCGAGGAAAAGAA30.10623229461756376No Hit
GCAGAGTACATGGGAAGCAGTGGTA30.10623229461756376No Hit
CTGTAGGACGTGGAATATGGCAAGA30.10623229461756376No Hit
GGTATCAACGCAGAGTACATGGGGG30.10623229461756376No Hit
GGTATCAACGCAGAGTACATGGGGA30.10623229461756376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA304.9421535E-419.2363641
AACGCAG400.003419487314.4272726
TCAACGC400.003419487314.4272724
TATCAAC400.003419487314.4272722
ATCAACG400.003419487314.4272723
CAACGCA400.003419487314.4272725
ACGCAGA450.008502128512.5952387
Produced by FastQC (version 0.11.5)