##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2824 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.77514164305949 30.0 25.0 31.0 10.0 33.0 2 26.12570821529745 30.0 26.0 31.0 10.0 34.0 3 26.44298866855524 30.0 26.0 31.0 10.0 34.0 4 30.183781869688385 35.0 30.0 35.0 16.0 37.0 5 29.44086402266289 35.0 28.0 35.0 7.0 37.0 6 29.40828611898017 35.0 29.0 37.0 2.0 37.0 7 29.18873937677054 35.0 28.0 37.0 2.0 37.0 8 29.003895184135978 35.0 28.0 37.0 2.0 37.0 9 29.812677053824363 35.0 27.0 39.0 2.0 39.0 10 29.674929178470254 35.0 27.0 39.0 2.0 39.0 11 29.789305949008497 35.0 27.0 39.0 2.0 39.0 12 29.301345609065155 35.0 27.0 39.0 2.0 39.0 13 29.40545325779037 35.0 27.0 39.0 2.0 39.0 14 29.64730878186969 36.0 27.0 40.0 2.0 41.0 15 29.695467422096318 36.0 27.0 40.0 2.0 41.0 16 29.60269121813031 36.0 27.0 39.0 2.0 41.0 17 29.695821529745043 36.0 27.0 39.0 2.0 41.0 18 29.617209631728045 36.0 27.0 40.0 2.0 41.0 19 29.76558073654391 37.0 27.0 40.0 2.0 41.0 20 29.880665722379604 37.0 27.0 40.0 2.0 41.0 21 29.59029745042493 37.0 27.0 40.0 2.0 41.0 22 29.534348441926344 37.0 27.0 40.0 2.0 41.0 23 28.921388101983002 36.0 24.0 40.0 2.0 41.0 24 28.90545325779037 36.0 25.0 40.0 2.0 41.0 25 28.655807365439095 36.0 22.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.4045105820105803 1101 2 -1.6828571428571522 1101 3 -1.9463624338624257 1101 4 -1.9390608465608494 1101 5 0.08203703703702558 1101 6 -3.2806613756613743 1101 7 -2.521283068783081 1101 8 -5.004748677248678 1101 9 -2.2940873015873002 1101 10 -0.03873015873016428 1101 11 -1.494589947089942 1101 12 -0.9322883597883589 1101 13 -1.7035714285714398 1101 14 -4.256164021164029 1101 15 -3.6147222222222197 1101 16 -0.20964285714286035 1101 17 -3.944060846560852 1101 18 -4.116044973544977 1101 19 -5.299179894179893 1101 20 -0.15072751322751188 1101 21 -5.206494708994708 1101 22 -5.790542328042328 1101 23 -4.235277777777775 1101 24 -3.9812830687830676 1101 25 -4.304841269841276 1102 1 -0.9283201058201058 1102 2 -1.0876190476190573 1102 3 -1.9225529100529002 1102 4 -0.7247751322751341 1102 5 -3.8227248677248795 1102 6 -6.471137566137564 1102 7 -8.402235449735464 1102 8 -4.361891534391535 1102 9 -7.2940873015873 1102 10 -8.324444444444449 1102 11 -10.423161375661369 1102 12 -7.860859788359786 1102 13 -6.417857142857155 1102 14 -8.089497354497361 1102 15 -5.8528174603174605 1102 16 -9.162023809523813 1102 17 -3.444060846560852 1102 18 -5.330330687830692 1102 19 -7.91822751322751 1102 20 -8.793584656084654 1102 21 -4.373161375661375 1102 22 -4.457208994708996 1102 23 -3.44956349206349 1102 24 -4.981283068783068 1102 25 -9.09055555555556 1103 1 0.6788227513227518 1103 2 2.7338095238095157 1103 3 1.7203042328042422 1103 4 4.060939153439151 1103 5 5.248703703703693 1103 6 5.136005291005294 1103 7 2.062050264550251 1103 8 3.245251322751322 1103 9 5.7059126984127 1103 10 2.7112698412698357 1103 11 5.755410052910058 1103 12 8.067711640211641 1103 13 7.29642857142856 1103 14 7.410502645502639 1103 15 7.468611111111112 1103 16 5.873690476190472 1103 17 6.805939153439148 1103 18 4.383955026455023 1103 19 5.8674867724867745 1103 20 4.599272486772488 1103 21 5.626838624338625 1103 22 5.542791005291004 1103 23 6.014722222222225 1103 24 5.018716931216932 1103 25 6.945158730158724 1104 1 3.178822751322752 1104 2 2.4838095238095157 1104 3 1.2203042328042422 1104 4 0.06093915343915057 1104 5 -1.7512962962963066 1104 6 -7.613994708994706 1104 7 0.8120502645502512 1104 8 -1.2547486772486778 1104 9 0.20591269841269977 1104 10 2.9612698412698357 1104 11 5.505410052910058 1104 12 2.817711640211641 1104 13 6.54642857142856 1104 14 6.410502645502639 1104 15 1.9686111111111124 1104 16 0.1236904761904718 1104 17 -4.444060846560852 1104 18 -0.6160449735449767 1104 19 2.6174867724867745 1104 20 0.5992724867724881 1104 21 6.876838624338625 1104 22 6.042791005291004 1104 23 6.764722222222225 1104 24 6.518716931216932 1104 25 7.695158730158724 1105 1 -9.642605820105821 1105 2 -7.373333333333342 1105 3 -5.493981481481473 1105 4 -6.153346560846565 1105 5 -5.965582010582022 1105 6 -4.185423280423279 1105 7 -6.830806878306891 1105 8 -6.933320105820105 1105 9 -4.2940873015873 1105 10 -4.610158730158737 1105 11 -5.708875661375657 1105 12 -3.5751455026455012 1105 13 -3.70357142857144 1105 14 -3.2323544973545033 1105 15 -6.138531746031745 1105 16 -5.447738095238101 1105 17 -8.015489417989425 1105 18 -5.330330687830692 1105 19 -5.775370370370368 1105 20 -5.650727513227512 1105 21 -6.373161375661375 1105 22 -4.028637566137569 1105 23 -2.1638492063492016 1105 24 -6.981283068783068 1105 25 -6.376269841269849 1106 1 -1.4045105820105803 1106 2 -1.1828571428571522 1106 3 3.88697089947091 1106 4 3.5609391534391506 1106 5 0.08203703703702558 1106 6 4.386005291005294 1106 7 -1.021283068783081 1106 8 4.495251322751322 1106 9 3.7059126984126998 1106 10 5.294603174603171 1106 11 1.3387433862433902 1106 12 -1.432288359788359 1106 13 -5.036904761904772 1106 14 -2.422830687830693 1106 15 0.05194444444444457 1106 16 -7.876309523809528 1106 17 3.8892724867724837 1106 18 4.050621693121688 1106 19 4.034153439153439 1106 20 5.349272486772488 1106 21 2.6268386243386246 1106 22 4.542791005291004 1106 23 0.5980555555555576 1106 24 4.018716931216932 1106 25 6.195158730158724 1107 1 4.678822751322752 1107 2 4.983809523809516 1107 3 0.47030423280424216 1107 4 4.310939153439151 1107 5 6.248703703703693 1107 6 6.386005291005294 1107 7 6.812050264550251 1107 8 5.995251322751322 1107 9 7.7059126984127 1107 10 7.461269841269836 1107 11 7.005410052910058 1107 12 7.317711640211641 1107 13 5.54642857142856 1107 14 7.910502645502639 1107 15 7.718611111111112 1107 16 7.373690476190472 1107 17 7.555939153439148 1107 18 7.883955026455023 1107 19 7.8674867724867745 1107 20 8.599272486772488 1107 21 9.126838624338625 1107 22 8.292791005291004 1107 23 9.014722222222225 1107 24 7.018716931216932 1107 25 7.945158730158724 1108 1 -1.0711772486772482 1108 2 -2.5161904761904843 1108 3 -2.5796957671957585 1108 4 -3.039060846560851 1108 5 -0.851296296296308 1108 6 -1.6139947089947064 1108 7 -1.887949735449748 1108 8 0.09525132275132364 1108 9 0.5059126984127005 1108 10 -0.23873015873016357 1108 11 -0.7945899470899427 1108 12 -1.6322883597883582 1108 13 0.29642857142856016 1108 14 -0.28949735449736025 1108 15 0.9186111111111117 1108 16 1.1236904761904718 1108 17 1.3559391534391487 1108 18 1.783955026455022 1108 19 0.7674867724867731 1108 20 0.7492724867724867 1108 21 0.226838624338626 1108 22 0.5427910052910043 1108 23 1.4647222222222247 1108 24 0.418716931216931 1108 25 0.9951587301587246 1109 1 3.678822751322752 1109 2 1.9838095238095157 1109 3 2.470304232804242 1109 4 3.3109391534391506 1109 5 -2.5012962962963066 1109 6 0.8860052910052936 1109 7 2.812050264550251 1109 8 3.995251322751322 1109 9 5.9559126984127 1109 10 -2.7887301587301643 1109 11 -2.744589947089942 1109 12 -5.432288359788359 1109 13 -2.95357142857144 1109 14 -1.839497354497361 1109 15 0.4686111111111124 1109 16 1.1236904761904718 1109 17 2.555939153439148 1109 18 4.633955026455023 1109 19 3.1174867724867745 1109 20 1.5992724867724881 1109 21 4.126838624338625 1109 22 -0.9572089947089957 1109 23 -0.2352777777777746 1109 24 2.7687169312169324 1109 25 -7.554841269841276 1110 1 -5.321177248677248 1110 2 0.9838095238095157 1110 3 0.47030423280424216 1110 4 1.0609391534391506 1110 5 -1.5012962962963066 1110 6 1.3860052910052936 1110 7 -3.437949735449749 1110 8 -10.504748677248678 1110 9 -10.7940873015873 1110 10 -10.538730158730164 1110 11 -10.494589947089942 1110 12 -10.432288359788359 1110 13 -10.70357142857144 1110 14 -11.339497354497361 1110 15 -10.781388888888888 1110 16 -9.876309523809528 1110 17 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1112 4 4.894272486772486 1112 5 4.748703703703693 1112 6 5.719338624338629 1112 7 1.8120502645502512 1112 8 6.495251322751322 1112 9 0.3725793650793676 1112 10 0.6279365079365036 1112 11 1.8387433862433902 1112 12 2.567711640211641 1112 13 2.629761904761896 1112 14 2.410502645502639 1112 15 1.8852777777777767 1112 16 2.9570238095238075 1112 17 0.7226058201058159 1112 18 0.8839550264550233 1112 19 2.7008201058201102 1112 20 2.0159391534391524 1112 21 2.9601719576719603 1112 22 3.5427910052910043 1112 23 4.264722222222225 1112 24 3.0187169312169324 1112 25 4.195158730158724 1113 1 3.428822751322752 1113 2 3.9838095238095157 1113 3 0.9703042328042422 1113 4 1.5609391534391506 1113 5 -2.7512962962963066 1113 6 -3.3639947089947064 1113 7 -1.4379497354497488 1113 8 1.2452513227513222 1113 9 -4.5440873015873 1113 10 -0.03873015873016428 1113 11 4.505410052910058 1113 12 -5.932288359788359 1113 13 -4.45357142857144 1113 14 -2.089497354497361 1113 15 -6.281388888888888 1113 16 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6.415370370370358 1202 6 6.719338624338629 1202 7 6.9787169312169155 1202 8 7.495251322751322 1202 9 8.7059126984127 1202 10 6.961269841269836 1202 11 8.672076719576722 1202 12 8.901044973544977 1202 13 6.629761904761896 1202 14 8.910502645502639 1202 15 9.718611111111112 1202 16 5.123690476190472 1202 17 7.222605820105812 1202 18 8.717288359788359 1202 19 9.034153439153439 1202 20 9.015939153439152 1202 21 9.626838624338625 1202 22 9.542791005291004 1202 23 9.264722222222225 1202 24 10.018716931216932 1202 25 9.195158730158724 1203 1 -0.9045105820105803 1203 2 0.6504761904761835 1203 3 -0.61302910052909 1203 4 -0.9390608465608494 1203 5 1.2487037037036934 1203 6 0.5526719576719614 1203 7 0.8120502645502512 1203 8 0.9952513227513222 1203 9 1.039246031746032 1203 10 1.7946031746031679 1203 11 2.505410052910058 1203 12 2.234378306878309 1203 13 0.963095238095228 1203 14 1.410502645502639 1203 15 1.3852777777777803 1203 16 2.123690476190472 1203 17 1.555939153439148 1203 18 2.5506216931216876 1203 19 0.03415343915344238 1203 20 2.682605820105824 1203 21 1.9601719576719603 1203 22 2.2094576719576686 1203 23 3.4313888888888897 1203 24 2.0187169312169324 1203 25 3.861825396825388 1204 1 3.178822751322752 1204 2 6.233809523809516 1204 3 4.970304232804242 1204 4 5.560939153439151 1204 5 6.248703703703693 1204 6 5.136005291005294 1204 7 6.312050264550251 1204 8 6.495251322751322 1204 9 8.2059126984127 1204 10 7.211269841269836 1204 11 5.255410052910058 1204 12 7.817711640211641 1204 13 5.54642857142856 1204 14 7.410502645502639 1204 15 4.468611111111112 1204 16 4.623690476190472 1204 17 6.055939153439148 1204 18 6.383955026455023 1204 19 5.1174867724867745 1204 20 4.349272486772488 1204 21 6.626838624338625 1204 22 8.542791005291004 1204 23 8.014722222222225 1204 24 7.768716931216932 1204 25 6.695158730158724 1205 1 2.5288227513227532 1205 2 0.683809523809515 1205 3 3.020304232804243 1205 4 -0.6390608465608487 1205 5 5.748703703703693 1205 6 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20 0.3492724867724881 2203 21 0.6268386243386246 2203 22 0.34279100529100504 2203 23 -2.335277777777776 2203 24 0.01871693121693241 2203 25 1.3951587301587232 2204 1 -1.6711772486772496 2204 2 2.4838095238095157 2204 3 1.2203042328042422 2204 4 2.3609391534391477 2204 5 1.3487037037036949 2204 6 -2.413994708994707 2204 7 -2.28794973544975 2204 8 -1.704748677248677 2204 9 -1.8940873015873017 2204 10 -1.2387301587301636 2204 11 -2.3945899470899406 2204 12 -0.2322883597883596 2204 13 -1.5035714285714405 2204 14 -0.8894973544973617 2204 15 -5.081388888888888 2204 16 -1.676309523809529 2204 17 -3.444060846560852 2204 18 -4.416044973544977 2204 19 -1.032513227513224 2204 20 -0.05072751322751046 2204 21 -1.3731613756613754 2204 22 0.7427910052910036 2204 23 1.0647222222222261 2204 24 0.6187169312169338 2204 25 -1.0048412698412754 2205 1 2.5288227513227532 2205 2 3.4838095238095157 2205 3 -2.1796957671957564 2205 4 -0.4390608465608494 2205 5 -3.451296296296306 2205 6 -1.8139947089947057 2205 7 -1.887949735449748 2205 8 -2.104748677248679 2205 9 -0.29408730158730023 2205 10 0.7612698412698364 2205 11 -2.7945899470899427 2205 12 -1.2322883597883596 2205 13 -2.1035714285714384 2205 14 -0.08949735449736096 2205 15 1.118611111111111 2205 16 -0.8763095238095282 2205 17 -1.2440608465608527 2205 18 -1.216044973544978 2205 19 -1.6325132275132255 2205 20 -3.4507275132275126 2205 21 0.6268386243386246 2205 22 1.5427910052910043 2205 23 1.8647222222222268 2205 24 1.6187169312169338 2205 25 2.3951587301587267 2206 1 -8.821177248677248 2206 2 -9.266190476190484 2206 3 -9.529695767195758 2206 4 -11.43906084656085 2206 5 -10.251296296296307 2206 6 -10.113994708994706 2206 7 -10.187949735449749 2206 8 -10.004748677248678 2206 9 -10.2940873015873 2206 10 -10.038730158730164 2206 11 -10.494589947089942 2206 12 -9.432288359788359 2206 13 -9.20357142857144 2206 14 -9.089497354497361 2206 15 -10.531388888888888 2206 16 -9.376309523809528 2206 17 -9.444060846560852 2206 18 -9.116044973544977 2206 19 -9.382513227513225 2206 20 -9.400727513227512 2206 21 -9.623161375661375 2206 22 -9.957208994708996 2206 23 -9.235277777777775 2206 24 -8.981283068783068 2206 25 -9.054841269841276 2207 1 3.678822751322752 2207 2 6.233809523809516 2207 3 5.470304232804242 2207 4 4.810939153439151 2207 5 6.748703703703693 2207 6 6.886005291005294 2207 7 3.812050264550251 2207 8 6.995251322751322 2207 9 7.4559126984127 2207 10 7.711269841269836 2207 11 8.505410052910058 2207 12 5.317711640211641 2207 13 6.54642857142856 2207 14 8.160502645502639 2207 15 7.718611111111112 2207 16 6.373690476190472 2207 17 8.805939153439148 2207 18 9.633955026455023 2207 19 9.617486772486775 2207 20 9.349272486772488 2207 21 9.126838624338625 2207 22 9.042791005291004 2207 23 6.764722222222225 2207 24 7.268716931216932 2207 25 5.695158730158724 2208 1 -4.471177248677247 2208 2 2.2838095238095164 2208 3 2.020304232804243 2208 4 2.5609391534391506 2208 5 -0.25129629629630656 2208 6 -1.6139947089947064 2208 7 3.71205026455025 2208 8 1.4952513227513222 2208 9 -1.2940873015873002 2208 10 1.3612698412698343 2208 11 -1.1945899470899413 2208 12 1.9677116402116397 2208 13 -2.3035714285714413 2208 14 -5.4894973544973595 2208 15 -0.08138888888888829 2208 16 2.923690476190469 2208 17 -4.044060846560853 2208 18 -9.216044973544978 2208 19 -9.632513227513225 2208 20 -7.850727513227511 2208 21 -6.373161375661375 2208 22 -3.2572089947089964 2208 23 -3.135277777777773 2208 24 -7.181283068783067 2208 25 -2.2048412698412747 2209 1 3.928822751322752 2209 2 3.817142857142848 2209 3 3.88697089947091 2209 4 5.227605820105815 2209 5 3.4153703703703577 2209 6 5.719338624338629 2209 7 3.312050264550251 2209 8 2.16191798941799 2209 9 1.3725793650793676 2209 10 1.6279365079365036 2209 11 -0.994589947089942 2209 12 7.234378306878305 2209 13 7.9630952380952245 2209 14 2.910502645502639 2209 15 6.051944444444448 2209 16 8.123690476190472 2209 17 5.889272486772484 2209 18 5.383955026455023 2209 19 5.3674867724867745 2209 20 3.349272486772488 2209 21 -3.0398280423280433 2209 22 2.5427910052910043 2209 23 1.2647222222222254 2209 24 5.685383597883597 2209 25 -5.471507936507944 2210 1 -4.321177248677248 2210 2 -9.516190476190484 2210 3 -11.779695767195758 2210 4 -9.18906084656085 2210 5 -13.251296296296307 2210 6 -9.613994708994706 2210 7 -5.437949735449749 2210 8 -9.504748677248678 2210 9 -10.2940873015873 2210 10 -9.538730158730164 2210 11 -10.994589947089942 2210 12 -7.432288359788359 2210 13 -9.20357142857144 2210 14 -9.839497354497361 2210 15 -15.281388888888888 2210 16 -4.626309523809528 2210 17 -12.194060846560852 2210 18 -7.616044973544977 2210 19 -4.8825132275132255 2210 20 -12.400727513227512 2210 21 -19.123161375661375 2210 22 -19.707208994708996 2210 23 -20.235277777777775 2210 24 -19.981283068783068 2210 25 -20.304841269841276 2211 1 -4.737843915343916 2211 2 -4.182857142857152 2211 3 -4.446362433862426 2211 4 -6.772394179894182 2211 5 -5.251296296296307 2211 6 -4.947328042328039 2211 7 -5.021283068783081 2211 8 -4.83808201058201 2211 9 -4.2940873015873 2211 10 -4.705396825396832 2211 11 -3.327923280423274 2211 12 -2.765621693121691 2211 13 -6.370238095238108 2211 14 -3.756164021164029 2211 15 -4.948055555555555 2211 16 -4.20964285714286 2211 17 -3.444060846560852 2211 18 -3.949378306878309 2211 19 -3.6325132275132255 2211 20 -7.984060846560844 2211 21 -5.039828042328043 2211 22 -5.790542328042328 2211 23 -4.735277777777775 2211 24 -3.3146164021163997 2211 25 -3.1381746031746083 2212 1 1.3288227513227504 2212 2 -0.11619047619048573 2212 3 1.8203042328042436 2212 4 3.7609391534391534 2212 5 3.148703703703692 2212 6 4.386005291005294 2212 7 5.512050264550254 2212 8 5.695251322751325 2212 9 6.905912698412703 2212 10 1.7612698412698364 2212 11 0.4054100529100566 2212 12 3.567711640211641 2212 13 4.496428571428563 2212 14 2.910502645502639 2212 15 0.7186111111111124 2212 16 0.3236904761904711 2212 17 2.555939153439148 2212 18 1.183955026455024 2212 19 2.767486772486773 2212 20 4.9492724867724895 2212 21 0.026838624338623163 2212 22 0.14279100529100575 2212 23 -0.335277777777776 2212 24 -0.7812830687830683 2212 25 -0.40484126984127755 2213 1 1.4288227513227518 2213 2 2.4838095238095157 2213 3 3.470304232804242 2213 4 3.8109391534391506 2213 5 3.2487037037036934 2213 6 3.6360052910052936 2213 7 1.3120502645502512 2213 8 1.9952513227513222 2213 9 4.9559126984127 2213 10 2.2112698412698357 2213 11 6.755410052910058 2213 12 7.067711640211641 2213 13 3.29642857142856 2213 14 4.910502645502639 2213 15 2.9686111111111124 2213 16 6.123690476190472 2213 17 7.555939153439148 2213 18 6.883955026455023 2213 19 5.1174867724867745 2213 20 7.599272486772488 2213 21 8.876838624338625 2213 22 3.7927910052910043 2213 23 7.014722222222225 2213 24 4.268716931216932 2213 25 5.195158730158724 2214 1 -5.237843915343916 2214 2 -5.3495238095238165 2214 3 -8.446362433862426 2214 4 -7.439060846560849 2214 5 -5.417962962962974 2214 6 -5.780661375661374 2214 7 -5.854616402116417 2214 8 -5.671415343915346 2214 9 -13.627420634920632 2214 10 -7.038730158730164 2214 11 -4.827923280423274 2214 12 -7.765621693121691 2214 13 -5.036904761904772 2214 14 -8.422830687830693 2214 15 -6.281388888888888 2214 16 -5.20964285714286 2214 17 -5.944060846560852 2214 18 -8.116044973544977 2214 19 -7.6325132275132255 2214 20 -6.150727513227512 2214 21 -5.373161375661375 2214 22 -7.457208994708996 2214 23 -13.568611111111107 2214 24 -8.481283068783068 2214 25 -6.138174603174608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 221.0 3 0.0 4 3.0 5 36.0 6 39.0 7 28.0 8 20.0 9 20.0 10 14.0 11 12.0 12 18.0 13 10.0 14 19.0 15 15.0 16 12.0 17 21.0 18 6.0 19 17.0 20 25.0 21 23.0 22 31.0 23 38.0 24 35.0 25 44.0 26 55.0 27 42.0 28 54.0 29 79.0 30 90.0 31 90.0 32 114.0 33 151.0 34 181.0 35 250.0 36 267.0 37 362.0 38 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.45799361928394 18.04324707550514 9.500177242112725 23.99858206309819 2 16.153028692879914 21.006021962451292 36.733970952887 26.106978391781794 3 18.307365439093484 27.939093484419264 28.966005665722378 24.78753541076487 4 12.677053824362606 19.36968838526912 31.444759206798867 36.508498583569406 5 12.039660056657224 38.668555240793204 32.507082152974505 16.78470254957507 6 30.00355492356914 36.43796658371845 14.71738357625311 18.841094916459298 7 24.458643947461837 36.38622648207313 18.849840255591054 20.305289314873978 8 27.29528535980149 33.002481389578165 17.795108117688763 21.907125132931586 9 26.019142148174407 15.278270116979794 19.248493442041827 39.45409429280397 10 20.219546742209634 28.966005665722378 27.053824362606232 23.760623229461757 11 31.444759206798867 25.424929178470258 18.6614730878187 24.468838526912183 12 24.575070821529746 28.505665722379604 27.089235127478755 19.8300283286119 13 31.975920679886688 23.406515580736546 24.71671388101983 19.90084985835694 14 23.273113708820404 23.804463336875664 28.30322352107687 24.619199433227063 15 27.90368271954674 24.858356940509914 27.372521246458923 19.86543909348442 16 20.467422096317282 28.222379603399432 30.73654390934844 20.573654390934845 17 20.325779036827196 26.770538243626063 30.240793201133144 22.6628895184136 18 18.342776203966004 22.981586402266288 34.73796033994334 23.937677053824363 19 19.723796033994333 22.875354107648725 39.05807365439094 18.342776203966004 20 21.458923512747877 19.086402266288953 39.553824362606235 19.90084985835694 21 20.96317280453258 20.361189801699716 39.09348441926346 19.58215297450425 22 20.077903682719548 21.635977337110482 39.12889518413598 19.157223796033996 23 19.29886685552408 20.361189801699716 39.48300283286119 20.856940509915013 24 19.688385269121813 22.13172804532578 38.491501416430594 19.688385269121813 25 18.590651558073652 22.202549575070822 38.562322946175634 20.644475920679888 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 9.0 2 7.0 3 5.0 4 5.0 5 5.0 6 15.0 7 25.0 8 25.0 9 25.0 10 26.5 11 28.0 12 28.0 13 28.0 14 20.0 15 12.0 16 12.0 17 12.0 18 32.0 19 52.0 20 52.0 21 52.0 22 72.5 23 93.0 24 93.0 25 93.0 26 139.5 27 186.0 28 186.0 29 186.0 30 216.0 31 246.0 32 246.0 33 246.0 34 250.5 35 255.0 36 255.0 37 255.0 38 229.0 39 203.0 40 203.0 41 203.0 42 224.5 43 246.0 44 246.0 45 246.0 46 277.0 47 308.0 48 308.0 49 308.0 50 318.0 51 328.0 52 328.0 53 328.0 54 295.0 55 262.0 56 262.0 57 262.0 58 226.5 59 191.0 60 191.0 61 191.0 62 176.0 63 161.0 64 161.0 65 161.0 66 135.5 67 110.0 68 110.0 69 110.0 70 84.5 71 59.0 72 59.0 73 59.0 74 43.0 75 27.0 76 27.0 77 27.0 78 20.5 79 14.0 80 14.0 81 14.0 82 8.5 83 3.0 84 3.0 85 3.0 86 2.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10623229461756376 2 0.03541076487252125 3 0.0 4 0.0 5 0.0 6 0.3895184135977337 7 0.24787535410764872 8 0.10623229461756376 9 0.10623229461756376 10 0.0 11 0.0 12 0.0 13 0.0 14 0.03541076487252125 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2824.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.74504249291785 #Duplication Level Percentage of deduplicated Percentage of total 1 96.44760213143873 76.91218130311614 2 2.0426287744227354 3.2577903682719547 3 0.3552397868561279 0.84985835694051 4 0.2220248667850799 0.708215297450425 5 0.08880994671403197 0.3541076487252125 6 0.2220248667850799 1.0623229461756374 7 0.04440497335701599 0.24787535410764872 8 0.0 0.0 9 0.04440497335701599 0.3186968838526912 >10 0.3996447602131439 6.763456090651558 >50 0.08880994671403197 5.559490084985836 >100 0.04440497335701599 3.9660056657223794 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 112 3.9660056657223794 No Hit GGTATCAACGCAGAGTACTTTTTTT 92 3.2577903682719547 No Hit TATCAACGCAGAGTACTTTTTTTTT 65 2.301699716713881 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 45 1.593484419263456 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 29 1.0269121813031163 No Hit ATCAACGCAGAGTACTTTTTTTTTT 21 0.7436260623229463 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 20 0.708215297450425 No Hit GTGGTATCAACGCAGAGTACTTTTT 20 0.708215297450425 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 18 0.6373937677053824 No Hit CCCATGTACTCTGCGTTGATACCAC 14 0.49575070821529743 No Hit GTACATGGGAAGCAGTGGTATCAAC 13 0.46033994334277617 No Hit GAGTACATGGGAAGCAGTGGTATCA 11 0.3895184135977337 No Hit CAACGCAGAGTACTTTTTTTTTTTT 9 0.3186968838526912 No Hit CATGTACTCTGCGTTGATACCACTG 7 0.24787535410764872 No Hit TCAACGCAGAGTACTTTTTTTTTTT 6 0.2124645892351275 No Hit AACGCAGAGTACTTTTTTTTTTTTT 6 0.2124645892351275 No Hit CAGTGGTATCAACGCAGAGTACATG 6 0.2124645892351275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 6 0.2124645892351275 No Hit GTATCAACGCAGAGTACATGGGAAG 6 0.2124645892351275 No Hit TATCAACGCAGAGTACATGGGAAGC 5 0.17705382436260625 No Hit GTTATCAACGCAGAGTACTTTTTTT 5 0.17705382436260625 No Hit CCACTGCTTCCCATGTACTCTGCGT 4 0.141643059490085 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4 0.141643059490085 No Hit GGGATCAACGCAGAGTACTTTTTTT 4 0.141643059490085 No Hit GTATCAACGCAGATTACTTTTTTTT 4 0.141643059490085 No Hit GCGTTGATACCACTGCTTCCCATGT 4 0.141643059490085 No Hit GTATCAACGCAGAGTACATGGGGGT 3 0.10623229461756376 No Hit TACCACTGCTTCCCATGTACTCTGC 3 0.10623229461756376 No Hit CGCAGAGTACTTTTTTTTTTTTTTT 3 0.10623229461756376 No Hit CATCTAAGTACCCCGAGGAAAAGAA 3 0.10623229461756376 No Hit GCAGAGTACATGGGAAGCAGTGGTA 3 0.10623229461756376 No Hit CTGTAGGACGTGGAATATGGCAAGA 3 0.10623229461756376 No Hit GGTATCAACGCAGAGTACATGGGGG 3 0.10623229461756376 No Hit GGTATCAACGCAGAGTACATGGGGA 3 0.10623229461756376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 30 4.9421535E-4 19.236364 1 AACGCAG 40 0.0034194873 14.427272 6 TCAACGC 40 0.0034194873 14.427272 4 TATCAAC 40 0.0034194873 14.427272 2 ATCAACG 40 0.0034194873 14.427272 3 CAACGCA 40 0.0034194873 14.427272 5 ACGCAGA 45 0.0085021285 12.595238 7 >>END_MODULE