FastQCFastQC Report
Thu 26 May 2016
SRR1926593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655908
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT604.2494867E-717.8186174
GTATCGT453.4519238E-416.1412146
GTCCTAA1003.2585376E-815.9313371
GCTACAC556.057702E-515.8566433
CCTATAC450.0046217314.5352563
AAAGGCG450.00639441113.84108455
TAAGACT703.595125E-513.7457914
TCTATAC806.9377493E-613.6268023
GACAGTC602.3124172E-413.5478837
AAAAGTG550.001573739713.2119455
GCAGTTC450.00974958712.9858138
GGTATCA1553.074092E-912.8478531
ATAGAAT600.00209548112.7183493
AAAGTGT1254.9149094E-912.4517956
CCTCTAG750.00254710112.3910411
TGATACC601.1332034E-412.16212917
AGTAGGA1255.317035E-611.9631562
TTCTATA800.00345847511.8951832
CTCTTAA907.4010715E-411.800991
TTTGAAC650.003817739711.7400153
Produced by FastQC (version 0.11.5)