FastQCFastQC Report
Thu 26 May 2016
SRR1926593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655908
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10950.1669441446056459No Hit
GGTATCAACGCAGAGTACTTTTTTT6830.10413045732023393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC2100.014.0756743
TAGGACG1450.013.8063264
AGGACGT1450.013.1488825
AGTACAG751.4238816E-512.7105865
GGACGTG1600.012.5119836
CCTACAC1301.8553692E-1012.4690213
TGTAGGA2550.012.344322
GTCCTAC1950.012.2254681
TATTCCC550.002986717512.1328325
CGTGGAA1459.640644E-1111.7932939
GTTATAC657.7681156E-411.7355493
TAGGTCT657.7831326E-411.7328494
CTGTAGG2250.011.4430381
ACGTGGA1501.7462298E-1011.4010558
GGACTGT1354.454705E-911.2982996
GTAAGAC600.005710366611.1243223
CTATACT600.0057201611.1217634
CTAAGGT600.0057201611.1217634
GTATAAC700.00144329510.8981311
TCAAGAC700.001444219510.8972963
Produced by FastQC (version 0.11.5)