##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 655908 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.566294358355137 31.0 28.0 31.0 23.0 34.0 2 29.013233563243627 31.0 28.0 33.0 23.0 34.0 3 29.364240106844253 31.0 30.0 33.0 25.0 34.0 4 33.1465022533648 35.0 33.0 37.0 28.0 37.0 5 32.85321112107186 35.0 33.0 37.0 26.0 37.0 6 32.925823438652984 35.0 33.0 37.0 26.0 37.0 7 32.75425516993237 35.0 33.0 37.0 26.0 37.0 8 32.7272315629631 35.0 33.0 37.0 25.0 37.0 9 33.99494136372784 38.0 34.0 39.0 25.0 39.0 10 33.9103517566488 37.0 34.0 39.0 25.0 39.0 11 34.14022545844844 38.0 34.0 39.0 26.0 39.0 12 33.938939607383965 37.0 34.0 39.0 25.0 39.0 13 34.086028833311985 38.0 34.0 39.0 26.0 39.0 14 34.777418479420895 39.0 34.0 40.0 24.0 41.0 15 34.897747854882084 39.0 34.0 40.0 25.0 41.0 16 34.86726949511212 39.0 34.0 40.0 25.0 41.0 17 34.76033529092495 39.0 34.0 40.0 25.0 41.0 18 34.731649865529924 39.0 34.0 40.0 25.0 41.0 19 34.78114308714027 39.0 34.0 40.0 24.0 41.0 20 34.76019655195546 39.0 34.0 40.0 24.0 41.0 21 34.68911188764278 39.0 34.0 40.0 24.0 41.0 22 34.62119992437964 39.0 34.0 40.0 23.0 41.0 23 34.290534648151876 39.0 34.0 40.0 18.0 41.0 24 34.356335034791464 39.0 34.0 41.0 19.0 41.0 25 34.159665684821654 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8312692273896936 1101 2 0.9738264966374288 1101 3 0.8900672889957661 1101 4 0.6792440990967705 1101 5 0.7656432513449403 1101 6 0.35715195685364876 1101 7 0.5094125047183695 1101 8 0.8141606518128981 1101 9 0.9634065941897063 1101 10 0.9448542122768799 1101 11 0.7038344008719193 1101 12 0.78682666332287 1101 13 0.75211361020299 1101 14 0.9706333407161054 1101 15 0.7725324864129064 1101 16 0.884860136035023 1101 17 0.8123021661802952 1101 18 0.8861824561378242 1101 19 0.7953563383698139 1101 20 0.7661945674899044 1101 21 0.7999469159768609 1101 22 0.8952781944006318 1101 23 1.280307174182994 1101 24 1.1757813526000902 1101 25 1.198753236183471 1102 1 0.2808994003831522 1102 2 0.4307615171154531 1102 3 0.3674650516951985 1102 4 0.1389176732761257 1102 5 0.39853340355473676 1102 6 -0.058839240652311275 1102 7 0.056967367485043496 1102 8 0.31459160725027147 1102 9 0.623416374738639 1102 10 0.4815434964629617 1102 11 0.28894657154249614 1102 12 0.34813542302700995 1102 13 0.2932744391045148 1102 14 0.6505404255012408 1102 15 0.5537416067747856 1102 16 0.18231108046927602 1102 17 0.3927471811567642 1102 18 0.6202951769642837 1102 19 0.5827333174027629 1102 20 0.4297406277625342 1102 21 0.2550541963729742 1102 22 0.378804082291083 1102 23 0.8482697635833247 1102 24 0.5926283481377155 1102 25 0.6474970469298427 1103 1 0.5756947733634519 1103 2 0.4284087299676038 1103 3 0.3539721447130795 1103 4 0.3085517012785246 1103 5 0.47245327786738045 1103 6 0.19559909399026765 1103 7 0.47197976678883435 1103 8 0.5849487996166118 1103 9 0.8529698457735577 1103 10 0.9716795037331067 1103 11 0.661500212008896 1103 12 0.7047401701890337 1103 13 0.5648122608317863 1103 14 0.8808447860514264 1103 15 0.5321028963023764 1103 16 0.7617089825994938 1103 17 0.6588085153526535 1103 18 0.7100407216864895 1103 19 0.7128813389051558 1103 20 0.7379880901410445 1103 21 0.6798196878012774 1103 22 0.4989849118892238 1103 23 0.8394924427571198 1103 24 0.8620028379179203 1103 25 0.8148760959571533 1104 1 0.16559608184584462 1104 2 0.29389072567903085 1104 3 0.16789987368994375 1104 4 0.016121224322411365 1104 5 0.15302203477133247 1104 6 -0.28664973073293254 1104 7 -0.08965974245849395 1104 8 -0.03705256341736174 1104 9 0.37974092104416 1104 10 0.10045221404003257 1104 11 0.09923131955711284 1104 12 0.0711975965330609 1104 13 -0.09243531721399734 1104 14 0.22371885349894427 1104 15 0.004019409715873223 1104 16 -0.07892182106165535 1104 17 -0.13648251960115942 1104 18 -0.20315048538436287 1104 19 -0.0655017280421788 1104 20 -0.2424322743723195 1104 21 -0.04509527368772126 1104 22 -0.14141212086741461 1104 23 -0.017528768892439928 1104 24 -0.12422704335305923 1104 25 -0.017893839925847033 1105 1 0.8793078238703167 1105 2 0.7255036847635417 1105 3 0.8980717071021473 1105 4 0.5623599519998166 1105 5 0.6495901434095757 1105 6 0.1298264826324882 1105 7 0.14622270443974372 1105 8 0.2858154169914471 1105 9 0.7179538893838782 1105 10 0.5930405826302447 1105 11 0.40568399184955695 1105 12 0.12155563878756936 1105 13 0.13832168427728675 1105 14 0.5099362046281541 1105 15 0.6841877351051551 1105 16 0.49007800428358195 1105 17 0.6524434730573745 1105 18 0.5623053411431584 1105 19 0.6667734263481506 1105 20 0.6784655406518709 1105 21 0.4775150536332049 1105 22 0.6420152324561812 1105 23 0.7623771265266797 1105 24 0.6579986255224171 1105 25 0.7035407772443563 1106 1 0.6017965800411567 1106 2 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0.4187678601571463 1107 14 0.916110600939831 1107 15 0.8893775716870636 1107 16 0.6379031857324335 1107 17 0.5388546372971135 1107 18 0.6608105278414769 1107 19 0.6500578788062725 1107 20 0.38357834706078364 1107 21 0.8032449858466535 1107 22 0.4944839068524871 1107 23 0.788894763583329 1107 24 0.7865976463833348 1107 25 0.9854904679824799 1108 1 1.21859300678409 1108 2 1.1633057437909287 1108 3 0.9383965645778218 1108 4 0.7960526655685669 1108 5 -0.8012243590526182 1108 6 0.35765492362868656 1108 7 0.565375941220637 1108 8 0.8028541044975697 1108 9 1.1201718617826089 1108 10 1.1614010902381935 1108 11 0.8131115865905798 1108 12 0.9577551837258298 1108 13 0.9539549059620356 1108 14 1.3489365203408497 1108 15 1.216969236518601 1108 16 1.135129842856415 1108 17 1.2108122182264225 1108 18 0.9524209935979187 1108 19 0.964220508183061 1108 20 1.0672617317778048 1108 21 1.2521803800329607 1108 22 1.3053914712656152 1108 23 1.6632114063932804 1108 24 1.2437037353505218 1108 25 1.2621744307660023 1109 1 0.5466187952891488 1109 2 -0.484455243795086 1109 3 0.5779430372928012 1109 4 0.7411867757844561 1109 5 -0.837525752600115 1109 6 0.07253671991025357 1109 7 0.5075231646915555 1109 8 0.6816980786629614 1109 9 1.103243352651738 1109 10 -1.2920923838714842 1109 11 -0.03495754135683171 1109 12 0.4939693604877391 1109 13 0.6168378241511476 1109 14 1.1254256412955428 1109 15 0.848534028600703 1109 16 0.8918103796814947 1109 17 0.7740482299219238 1109 18 0.9501656520500674 1109 19 0.7904178179102246 1109 20 0.7748157812108971 1109 21 0.9219069342783399 1109 22 0.7885232838763159 1109 23 0.7822773030932595 1109 24 0.7659017520331304 1109 25 1.0082698466978641 1110 1 0.6787979407720641 1110 2 -0.4919432801079573 1110 3 0.20743081977393274 1110 4 0.41808136842184496 1110 5 0.6103006264901225 1110 6 0.21900509919459665 1110 7 0.6532375143970341 1110 8 0.7137928624207177 1110 9 0.8765566780714948 1110 10 0.8019970694197411 1110 11 0.570946286276488 1110 12 0.8365750179492721 1110 13 0.6783621584027557 1110 14 0.9020761075247279 1110 15 0.8750295828124379 1110 16 0.5534302741173533 1110 17 0.7373247497394644 1110 18 0.7804268747724876 1110 19 0.8301350194899584 1110 20 0.9682206353696259 1110 21 0.8057863791333943 1110 22 0.7963622052407402 1110 23 0.9838495667497824 1110 24 0.9747231515418946 1110 25 1.1491016682392186 1111 1 0.39766672853960827 1111 2 0.5644255953438133 1111 3 0.5063779076054651 1111 4 0.4555268190914816 1111 5 0.9173427219326484 1111 6 0.32830484908021873 1111 7 0.7219515492509316 1111 8 0.6193370774832303 1111 9 0.9920387503497992 1111 10 1.000928023353957 1111 11 0.642325921556882 1111 12 0.6472869904702065 1111 13 0.6441372518741417 1111 14 1.0094561574540677 1111 15 0.9431146294955184 1111 16 0.8546579312026665 1111 17 0.7716657891521592 1111 18 0.8056417400934208 1111 19 0.598666593215242 1111 20 0.6896342141876133 1111 21 0.8155805121624482 1111 22 0.5515104383451543 1111 23 0.94054758929515 1111 24 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2208 15 1.0503138721221106 2208 16 0.9709530663090788 2208 17 0.9979961219853308 2208 18 1.1626805163256506 2208 19 0.9081562751212076 2208 20 1.1754271698877048 2208 21 1.2364625389474 2208 22 1.1192573223604683 2208 23 1.5344550403896022 2208 24 1.2985852988613686 2208 25 1.365807206445325 2209 1 0.11766522799566204 2209 2 0.2093179625769359 2209 3 0.718675959399242 2209 4 1.0679699234668192 2209 5 1.1050646773915034 2209 6 1.0063686159457035 2209 7 1.365090937279092 2209 8 1.1041987773494313 2209 9 1.4219766340826538 2209 10 0.8959790418481575 2209 11 1.0517993556767493 2209 12 1.2423573909903993 2209 13 1.1557172308665287 2209 14 1.3836925994142817 2209 15 1.4674429797724997 2209 16 1.3015425831389962 2209 17 1.4929092711643932 2209 18 1.4517501731504012 2209 19 1.5271078406674405 2209 20 1.4491056163208853 2209 21 1.4589348218497094 2209 22 1.5136725033437202 2209 23 1.7278845614475529 2209 24 1.5196497058798997 2209 25 1.5063833932303794 2210 1 -0.19341137655920448 2210 2 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0.2517075590213125 2211 15 -0.03692869643421659 2211 16 0.13512202941025464 2211 17 0.1453394461329296 2211 18 0.21524578878796063 2211 19 0.2741088149702051 2211 20 0.05016669292544407 2211 21 0.17918560332171296 2211 22 0.4097392633810628 2211 23 0.6346204442064547 2211 24 0.45202193013192016 2211 25 0.6068685148152468 2212 1 1.1170245856449306 2212 2 0.40036996849931583 2212 3 0.66319455226391 2212 4 0.6811625290304377 2212 5 0.8629321213708891 2212 6 0.8097679354411653 2212 7 0.7733651891511926 2212 8 0.8168457691776823 2212 9 1.2759617854166123 2212 10 0.8973777776472573 2212 11 0.7912145203930123 2212 12 1.0112812542061391 2212 13 0.9674911695356982 2212 14 1.1698266075575603 2212 15 1.0381503201101836 2212 16 0.1862145030729394 2212 17 0.7850368444111666 2212 18 0.9787052000573766 2212 19 0.8893234666462035 2212 20 1.158081371567036 2212 21 1.448141777801986 2212 22 1.3179865177510521 2212 23 1.5067383810996162 2212 24 1.2033752546968444 2212 25 0.6245676704434402 2213 1 0.19133430528066597 2213 2 -0.08480470304416343 2213 3 -0.6478814307433645 2213 4 -1.2395708827833651 2213 5 -2.067746524722871 2213 6 -0.7959661260019573 2213 7 -0.7263237762058488 2213 8 -0.2996052074317248 2213 9 -1.1215542683018285 2213 10 -1.2372765998700572 2213 11 -1.1498503204871753 2213 12 -1.224471193715246 2213 13 -1.2818916826126028 2213 14 -8.301396782247593 2213 15 -4.056280191207144 2213 16 -2.48737038773028 2213 17 -1.8905914258818726 2213 18 -6.153988478065074 2213 19 -3.3662840155086755 2213 20 -5.848487758835223 2213 21 -2.9506361316514997 2213 22 -2.5191848780858095 2213 23 -5.424902897528369 2213 24 -3.134735150789961 2213 25 -2.648542400385839 2214 1 0.3358488468923646 2214 2 0.316736420204613 2214 3 -1.7014715266527212 2214 4 -1.8760625449231334 2214 5 -0.3975701811473371 2214 6 -0.49963770606412794 2214 7 -0.7013809520609762 2214 8 -0.6974213408374901 2214 9 -5.71042449166427 2214 10 -1.744644410662488 2214 11 -1.0564836326696252 2214 12 -1.3235326423528377 2214 13 -1.7115391324096976 2214 14 -3.0522310247645166 2214 15 -2.5173070284807793 2214 16 -2.0036078740925234 2214 17 -2.040634108057162 2214 18 -1.2730009905615915 2214 19 -4.530578435784307 2214 20 -2.1937317776245067 2214 21 -3.9480569413802975 2214 22 -6.273675636367749 2214 23 -6.089441542015521 2214 24 -5.203275420395233 2214 25 -3.1851669022369222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19723.0 3 17.0 4 314.0 5 2344.0 6 2903.0 7 2358.0 8 1796.0 9 1478.0 10 1334.0 11 1165.0 12 1164.0 13 1163.0 14 1164.0 15 1253.0 16 1259.0 17 1291.0 18 1408.0 19 1696.0 20 2171.0 21 2877.0 22 3833.0 23 4595.0 24 5637.0 25 6576.0 26 7674.0 27 8592.0 28 9872.0 29 12116.0 30 14694.0 31 18910.0 32 24331.0 33 31175.0 34 41180.0 35 54785.0 36 74503.0 37 110239.0 38 178318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97068374085965 18.950889314794242 8.652355432938217 30.426071511407887 2 14.315060658975318 21.460085555462356 37.66910929290927 26.555744492653062 3 18.367530570103064 24.023724047057677 28.820538473035175 28.78820690980409 4 12.265962297759552 17.0151147522483 36.69387860921709 34.02504434077506 5 13.459060417012958 38.05788407021482 34.806232167610354 13.676823345161864 6 32.57937248828504 37.6718219632161 16.96427205029398 12.784533498204878 7 28.80838355369186 31.802204349121237 20.990811392956164 18.39860070423073 8 26.079297980808747 36.96905902968213 19.366789444657474 17.58485354485165 9 26.089201712961334 15.734127378200535 19.32627391186774 38.85039699697039 10 14.198821313937621 30.275458780296272 33.78879714553647 21.73692276022964 11 35.211787270504075 23.145203340783723 22.800483004875787 18.842526383836415 12 22.340806696295846 25.555242153731218 31.101336362319614 21.00261478765332 13 28.599590186428586 21.842697451471853 25.415454606438708 24.142257755660854 14 20.907332137146483 21.71628400893478 27.458896334541727 29.917487519377005 15 23.24004647064025 30.02905939643054 23.12600434823707 23.604889784692134 16 22.82655429078479 28.918544764855298 25.928427459315074 22.32647348504484 17 22.089762960771967 28.55082385120517 26.890009346179124 22.469403841843743 18 21.865166003951895 28.40988095528505 27.77044251457566 21.9545105261874 19 22.989397741722037 27.635242065077193 27.211243211596926 22.164116981603847 20 22.96954256676714 27.548753695309795 27.62330785684991 21.858395881073154 21 23.03127877690164 27.057758100221374 27.18414777682236 22.72681534605463 22 22.552553101959422 27.55188227617288 27.380516779792284 22.515047842075415 23 22.533834827193488 27.423095042437417 27.115581934634026 22.927488195735066 24 22.259168629603373 27.384606002393642 27.835281023928772 22.520944344074216 25 22.592345267933915 27.26830592095233 27.37457082395702 22.764777987156734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 173.5 3 194.0 4 194.0 5 194.0 6 320.0 7 446.0 8 446.0 9 446.0 10 781.0 11 1116.0 12 1116.0 13 1116.0 14 1843.5 15 2571.0 16 2571.0 17 2571.0 18 4488.5 19 6406.0 20 6406.0 21 6406.0 22 10165.5 23 13925.0 24 13925.0 25 13925.0 26 19728.0 27 25531.0 28 25531.0 29 25531.0 30 33527.5 31 41524.0 32 41524.0 33 41524.0 34 49376.5 35 57229.0 36 57229.0 37 57229.0 38 64586.0 39 71943.0 40 71943.0 41 71943.0 42 77667.5 43 83392.0 44 83392.0 45 83392.0 46 86484.5 47 89577.0 48 89577.0 49 89577.0 50 87805.5 51 86034.0 52 86034.0 53 86034.0 54 77260.0 55 68486.0 56 68486.0 57 68486.0 58 58468.5 59 48451.0 60 48451.0 61 48451.0 62 39521.0 63 30591.0 64 30591.0 65 30591.0 66 23535.5 67 16480.0 68 16480.0 69 16480.0 70 12035.5 71 7591.0 72 7591.0 73 7591.0 74 5182.5 75 2774.0 76 2774.0 77 2774.0 78 1909.0 79 1044.0 80 1044.0 81 1044.0 82 685.5 83 327.0 84 327.0 85 327.0 86 213.5 87 100.0 88 100.0 89 100.0 90 61.0 91 22.0 92 22.0 93 22.0 94 11.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08720735225062051 2 0.029424858364282797 3 0.030797002018575777 4 0.02911993755221769 5 0.022716600498850447 6 0.37657720290040675 7 0.19713130500009146 8 0.20414448367758894 9 0.20871829585856552 10 0.014178817761027461 11 0.002134445684455747 12 0.003506589338748727 13 0.0 14 0.07501051976801625 15 0.0015246040603255333 16 0.002134445684455747 17 0.003811510150813834 18 0.0030492081206510667 19 0.0015246040603255333 20 0.0013721436542929802 21 0.0 22 0.0 23 1.5246040603255335E-4 24 4.5738121809766E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 655908.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.84772334496047 #Duplication Level Percentage of deduplicated Percentage of total 1 93.0785137691411 82.6981404072074 2 5.471139999857808 9.721966661778273 3 0.7850986213384697 2.0926267532157072 4 0.26600971073176877 0.9453742874467662 5 0.1220306907420217 0.5421074525320787 6 0.07781983798008732 0.41484692613626667 7 0.04618838064284317 0.287261272557494 8 0.02908439836851371 0.20672660639202606 9 0.0197936862556356 0.15827615643758924 >10 0.09348238571621517 1.4244845592278343 >50 0.0063655600981098286 0.4033829045058205 >100 0.003956848459738211 0.7561532022137094 >500 3.4407377852959867E-4 0.18128111927836418 >1k 1.7203688926479933E-4 0.16737169107066507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1095 0.1669441446056459 No Hit GGTATCAACGCAGAGTACTTTTTTT 683 0.10413045732023393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5246040603255335E-4 0.0 10 0.0 0.0 0.0 1.5246040603255335E-4 0.0 11 0.0 0.0 0.0 1.5246040603255335E-4 0.0 12 0.0 0.0 0.0 1.5246040603255335E-4 0.0 13 0.0 0.0 0.0 1.5246040603255335E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGGAC 210 0.0 14.075674 3 TAGGACG 145 0.0 13.806326 4 AGGACGT 145 0.0 13.148882 5 AGTACAG 75 1.4238816E-5 12.710586 5 GGACGTG 160 0.0 12.511983 6 CCTACAC 130 1.8553692E-10 12.469021 3 TGTAGGA 255 0.0 12.34432 2 GTCCTAC 195 0.0 12.225468 1 TATTCCC 55 0.0029867175 12.132832 5 CGTGGAA 145 9.640644E-11 11.793293 9 GTTATAC 65 7.7681156E-4 11.735549 3 TAGGTCT 65 7.7831326E-4 11.732849 4 CTGTAGG 225 0.0 11.443038 1 ACGTGGA 150 1.7462298E-10 11.401055 8 GGACTGT 135 4.454705E-9 11.298299 6 GTAAGAC 60 0.0057103666 11.124322 3 CTATACT 60 0.00572016 11.121763 4 CTAAGGT 60 0.00572016 11.121763 4 GTATAAC 70 0.001443295 10.898131 1 TCAAGAC 70 0.0014442195 10.897296 3 >>END_MODULE