FastQCFastQC Report
Thu 26 May 2016
SRR1926592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584298
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA601.0177264E-619.9957521
AGGTGTA350.001375329217.4307337
GTCCTAC1650.016.1581841
GCTATAT500.00244046915.9966021
ACACCGT400.00290432815.5860976
TAACTCA506.0959073E-414.9769944
TATTGTA657.0475106E-414.6916322
AGTAAGC657.0475106E-414.6916322
GTAGGAC1750.013.7218763
TATAGGA1006.842485E-613.642232
GTCTGGA700.001469638813.33050251
GTCCTAT700.001469638813.33050251
CCTCTAG600.008192793513.3305021
TAGGACC1055.7461875E-813.2449614
TGTAGGA1653.6743586E-1013.228832
CTGCACT652.942757E-413.1665874
GTAACCC500.009206451513.0981533
TGGACAG902.721641E-612.702765
AGGACGT2150.012.56848055
TCCTACA2500.012.5508532
Produced by FastQC (version 0.11.5)