##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584298 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.33136173664808 27.0 7.0 31.0 2.0 31.0 2 16.468278173123988 16.0 2.0 30.0 2.0 31.0 3 19.925149495634077 23.0 15.0 31.0 2.0 31.0 4 21.930711725865912 28.0 2.0 35.0 2.0 37.0 5 22.898599687145943 30.0 2.0 35.0 2.0 37.0 6 23.353138295869574 30.0 2.0 35.0 2.0 37.0 7 23.293410211912416 30.0 7.0 35.0 2.0 37.0 8 23.569699023443516 31.0 10.0 35.0 2.0 37.0 9 26.245888228267084 32.0 17.0 37.0 2.0 39.0 10 26.985035033493183 32.0 23.0 37.0 2.0 39.0 11 24.114184885109996 30.0 7.0 37.0 2.0 39.0 12 25.237264204224555 31.0 11.0 37.0 2.0 39.0 13 21.903819626286587 27.0 2.0 37.0 2.0 39.0 14 25.23551509674858 31.0 17.0 38.0 2.0 40.0 15 26.341036594340558 31.0 17.0 38.0 2.0 40.0 16 26.69806331700605 31.0 17.0 39.0 2.0 40.0 17 26.008480261784225 31.0 11.0 38.0 2.0 40.0 18 26.078663627121777 31.0 17.0 38.0 2.0 40.0 19 26.48884644479358 31.0 18.0 39.0 2.0 40.0 20 26.742039164946654 31.0 18.0 39.0 2.0 40.0 21 24.58249386443219 30.0 2.0 38.0 2.0 40.0 22 25.51007533826917 31.0 2.0 38.0 2.0 40.0 23 25.503267168465406 31.0 2.0 38.0 2.0 40.0 24 24.58374151545958 31.0 2.0 39.0 2.0 40.0 25 24.371271166425352 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.089221320097494 1101 2 9.84493017820357 1101 3 6.356356979985364 1101 4 7.672848408974222 1101 5 7.481515778889094 1101 6 7.1565206072578 1101 7 6.6517745800688886 1101 8 5.204059047726513 1101 9 4.7811285280406715 1101 10 4.769145303054447 1101 11 7.7024295268716365 1101 12 5.2689098858569885 1101 13 9.78238805868229 1101 14 7.068537877837073 1101 15 6.043874296662004 1101 16 5.675449033208171 1101 17 1.9437559424810438 1101 18 4.184625235315512 1101 19 4.85319889693432 1101 20 2.983705312183467 1101 21 0.3163969411467491 1101 22 3.9908251635257237 1101 23 5.181314483114285 1101 24 6.222225265057634 1101 25 7.011602602324242 1102 1 5.222102995393918 1102 2 10.265274704690286 1102 3 6.233957396664799 1102 4 7.382543865330113 1102 5 7.766753531271213 1102 6 6.3029826618550615 1102 7 6.31701784732585 1102 8 6.524346893555123 1102 9 5.57131051891049 1102 10 4.888630428211467 1102 11 7.6023996546329045 1102 12 4.614826580608664 1102 13 9.27160341454491 1102 14 6.932688127538352 1102 15 4.844396677862406 1102 16 5.280093783353703 1102 17 5.9907128039044935 1102 18 6.069425627484389 1102 19 4.7966216427292245 1102 20 5.081399022162326 1102 21 3.8807256583255842 1102 22 4.856350302163548 1102 23 5.420051117202341 1102 24 7.164989289690745 1102 25 6.806491385681575 1103 1 4.246310906904981 1103 2 8.50873645947094 1103 3 5.322696304301342 1103 4 6.938474374016529 1103 5 6.625036870853766 1103 6 6.1330507783405 1103 7 6.091733507868284 1103 8 5.384965878681982 1103 9 4.811592983602402 1103 10 3.952341214363198 1103 11 5.031744424036731 1103 12 3.8959789365641697 1103 13 7.65679745279013 1103 14 5.9772294348749355 1103 15 5.047066656615062 1103 16 4.705407806600796 1103 17 2.8520035800574774 1103 18 1.34890631739815 1103 19 3.3338826101083043 1103 20 3.8779791371479533 1103 21 6.085176664449818 1103 22 1.8148554890151942 1103 23 4.063517558897129 1103 24 5.429315623898965 1103 25 1.4451459288636777 1104 1 5.3204498343206055 1104 2 10.142491660580509 1104 3 6.72761231601347 1104 4 7.295979382492408 1104 5 7.4936838118308025 1104 6 7.029691246825628 1104 7 6.6647378228628895 1104 8 6.748490689900969 1104 9 5.652030647341036 1104 10 4.460828654646754 1104 11 7.605929583537424 1104 12 4.847639438952829 1104 13 8.875746828656983 1104 14 6.638930005070652 1104 15 5.890746345319023 1104 16 5.53008996101596 1104 17 6.034094426104634 1104 18 3.7966062711660626 1104 19 4.685477617043116 1104 20 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2204 4 5.579423179265039 2204 5 1.29163746473154 2204 6 3.7517408270011643 2204 7 4.973290260507309 2204 8 5.210742622614944 2204 9 3.489647489766604 2204 10 2.8259342526232274 2204 11 4.516711892904787 2204 12 4.402778603868246 2204 13 2.7740057219939906 2204 14 3.7200916303571105 2204 15 3.9498745558113058 2204 16 3.8938814085783164 2204 17 -1.2400542763857878 2204 18 2.408727454471979 2204 19 3.1941736413102078 2204 20 3.6378677073327985 2204 21 4.96784207962013 2204 22 4.5463764846155215 2204 23 3.2160757418177006 2204 24 4.799758544782634 2204 25 4.91425107010398 2205 1 5.3720958983209 2205 2 9.954368744643075 2205 3 6.671450859887017 2205 4 7.092889903669249 2205 5 7.141790884287097 2205 6 6.620234899300712 2205 7 6.56788256964353 2205 8 6.76187658920275 2205 9 5.408623193535838 2205 10 4.548689968918783 2205 11 6.9755469450451315 2205 12 5.8525803943515875 2205 13 9.21983960513494 2205 14 6.5586238205862095 2205 15 5.419222239278234 2205 16 5.090669169210354 2205 17 5.500333073145033 2205 18 5.644232512392275 2205 19 4.1636467738383125 2205 20 4.43621345237942 2205 21 -1.2240290070661004 2205 22 3.2500193395016233 2205 23 4.2272927463914804 2205 24 6.109159604501961 2205 25 6.283125206596274 2206 1 4.448096621190693 2206 2 9.080522173756654 2206 3 6.487140748745787 2206 4 2.0005378660800197 2206 5 5.851862267679163 2206 6 6.172733318023038 2206 7 6.314511285646063 2206 8 6.267307148523251 2206 9 4.907148539157959 2206 10 3.952738039760021 2206 11 3.7097206145129213 2206 12 4.831772587357822 2206 13 8.129773643266319 2206 14 0.11000721265271451 2206 15 2.4353603074087147 2206 16 3.395526854219842 2206 17 4.5356940562479515 2206 18 -1.0079984445066117 2206 19 0.9757873720130661 2206 20 2.5656378673066804 2206 21 3.5641846009577556 2206 22 -2.1879222887625858 2206 23 -3.310768155388587 2206 24 0.9582045127878551 2206 25 2.364352278070026 2207 1 1.4768432214765 2207 2 -13.910906397671917 2207 3 -6.227396659634795 2207 4 -4.297565669622742 2207 5 1.254345096241373 2207 6 1.9637949508078094 2207 7 3.206837842599917 2207 8 3.0505367617625474 2207 9 0.7419929590168977 2207 10 0.3534479461814506 2207 11 -1.5734536813428868 2207 12 -2.525641324964127 2207 13 0.27744985218163976 2207 14 -4.959284263860447 2207 15 -1.4268947979248026 2207 16 -0.42745475275014755 2207 17 0.8107661993355855 2207 18 0.4090697341685363 2207 19 0.10645901724424789 2207 20 -0.031161100102160333 2207 21 -4.075890077509953 2207 22 -4.614359388287777 2207 23 -1.8003555799034068 2207 24 -0.014160766334441632 2207 25 0.6282503096931293 2208 1 5.302825365055927 2208 2 6.549632135290013 2208 3 6.538927457858403 2208 4 8.641938582355387 2208 5 3.9374677856523697 2208 6 6.4480350821827415 2208 7 6.924873402637704 2208 8 7.004646318174398 2208 9 5.43035320821221 2208 10 5.030175365665315 2208 11 7.104773008729659 2208 12 6.594109767355167 2208 13 9.150919683855257 2208 14 3.217419336276535 2208 15 5.026061991982264 2208 16 5.79324273165717 2208 17 6.14959775700402 2208 18 1.0855922173848853 2208 19 3.545913383420416 2208 20 4.573957273063414 2208 21 7.304439276644185 2208 22 6.221535198967885 2208 23 1.8160046261474285 2208 24 3.1872852927321453 2208 25 4.600062584476714 2209 1 2.642434600284769 2209 2 4.981370025092309 2209 3 5.037611132021048 2209 4 0.3474948927931436 2209 5 4.621020223544434 2209 6 5.113790228592144 2209 7 5.779064131174518 2209 8 4.208305987083065 2209 9 3.956289073420443 2209 10 3.2259087714673385 2209 11 -0.532642916265246 2209 12 2.9965402993206567 2209 13 1.8397968720700355 2209 14 3.416011858413455 2209 15 3.8516785420196307 2209 16 3.6102423013743135 2209 17 1.2973433013118303 2209 18 3.4188830885944306 2209 19 3.8812112976808955 2209 20 3.8332394933229423 2209 21 6.333313520818383 2209 22 4.954615458043456 2209 23 0.3219380002444012 2209 24 -2.979559714854421 2209 25 -0.4739404048568012 2210 1 -2.316465948023037 2210 2 -13.910906397671917 2210 3 -7.6730585868023855 2210 4 -2.458997017640911 2210 5 -0.7214932140484116 2210 6 -1.3472002367942366 2210 7 -0.965621604719388 2210 8 -20.91364523242913 2210 9 -12.863748470808819 2210 10 -6.88934390929867 2210 11 -9.888175288034144 2210 12 -4.555802163472745 2210 13 -5.14116766348894 2210 14 -3.955264105176745 2210 15 -5.319789194252415 2210 16 -4.894329181217586 2210 17 -4.560717903884939 2210 18 -4.609703870863203 2210 19 -10.241421930312516 2210 20 -6.990906983191657 2210 21 -5.157786606129733 2210 22 -9.411288844687279 2210 23 -7.4481103480796165 2210 24 -10.5998464285189 2210 25 -8.048117267887891 2211 1 -0.2559235538575173 2211 2 4.745376053003058 2211 3 0.4780886793198853 2211 4 1.2280827407276966 2211 5 1.1821129723223898 2211 6 -0.24523137564258946 2211 7 -20.672155381020605 2211 8 -13.780727267735463 2211 9 -9.85069747775348 2211 10 -7.814032469066561 2211 11 -9.910409929916689 2211 12 -5.42472644839355 2211 13 -7.617596183168928 2211 14 -5.536214416185739 2211 15 -6.06624182877286 2211 16 -12.106591525842461 2211 17 -8.218321668421979 2211 18 -6.403189069043034 2211 19 -9.209137713285852 2211 20 -7.371807615559362 2211 21 -5.205905890067317 2211 22 -6.7703136253595275 2211 23 -8.874918874866406 2211 24 -22.015128820545478 2211 25 -21.788981055263307 2212 1 -1.1327224879384268 2212 2 -13.910906397671917 2212 3 -7.73885648330096 2212 4 -19.414700229158075 2212 5 -20.25194725613036 2212 6 -20.660600015310294 2212 7 -9.848689288447947 2212 8 -9.487380431567988 2212 9 -5.254333853583855 2212 10 -5.593584170501909 2212 11 -5.386322237504096 2212 12 -2.8166942740907395 2212 13 -6.230418063125601 2212 14 -3.7624014192607618 2212 15 -3.6781616568129287 2212 16 -3.899220185607927 2212 17 -4.053991729799513 2212 18 -3.147832367311473 2212 19 -3.8025928627503767 2212 20 -3.7720872851870375 2212 21 -1.7339762478812446 2212 22 -3.0359975360843343 2212 23 -3.0866182758458116 2212 24 -1.9720717699534447 2212 25 -2.417613993907011 2213 1 -18.828093854999782 2213 2 -13.910906397671917 2213 3 -8.794061265099398 2213 4 -6.3783566168232735 2213 5 -20.25194725613036 2213 6 -10.862141865530559 2213 7 -20.672155381020605 2213 8 -10.86408576106349 2213 9 -8.786879151213345 2213 10 -7.684633472721622 2213 11 -6.868389307870128 2213 12 -5.532286569344507 2213 13 -19.137845404352728 2213 14 -12.618943909646427 2213 15 -7.6364039048841335 2213 16 -6.78249076692553 2213 17 -5.320471246877702 2213 18 -5.421573019711143 2213 19 -6.104758044391382 2213 20 -6.333950972634582 2213 21 -4.466004197028401 2213 22 -5.288100597971731 2213 23 -5.035024501098675 2213 24 -3.920415164157813 2213 25 -4.978408368038636 2214 1 -18.828093854999782 2214 2 -13.910906397671917 2214 3 -12.166735234425971 2214 4 -19.414700229158075 2214 5 -20.25194725613036 2214 6 -20.660600015310294 2214 7 -16.435279580764522 2214 8 -14.950777114631434 2214 9 -13.816956088608642 2214 10 -13.836591879147361 2214 11 -14.310662288968574 2214 12 -12.497150039311284 2214 13 -19.137845404352728 2214 14 -16.69104028445112 2214 15 -23.81702064497224 2214 16 -18.184473145780156 2214 17 -23.531924991371096 2214 18 -17.7027597395496 2214 19 -14.858087391720852 2214 20 -13.495121842467112 2214 21 -11.078884724083402 2214 22 -12.10560413743035 2214 23 -12.024038077954282 2214 24 -10.898611535014108 2214 25 -10.598200005327326 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 105315.0 3 4.0 4 410.0 5 1790.0 6 2487.0 7 2508.0 8 2160.0 9 2049.0 10 1914.0 11 1745.0 12 1710.0 13 1835.0 14 2661.0 15 6039.0 16 8548.0 17 9264.0 18 9441.0 19 11043.0 20 12947.0 21 15111.0 22 15847.0 23 16965.0 24 18161.0 25 18641.0 26 15639.0 27 12434.0 28 13323.0 29 15225.0 30 14749.0 31 17290.0 32 22147.0 33 27649.0 34 33629.0 35 38353.0 36 37602.0 37 39022.0 38 28641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.861524805207615 18.6209685373311 11.58753328730644 25.929973370154848 2 14.328401524149385 20.250871978975766 40.01133975740443 25.40938673947042 3 18.61244862114874 25.146439942822578 28.842629507398737 27.39848192862994 4 11.946438782641845 16.837751763136158 38.58131075671898 32.63449869750302 5 12.522467122369443 37.39893502379686 36.86446961772772 13.214128236105976 6 31.130924674574256 37.56721185175674 18.83035327351813 12.471510200150876 7 28.393519819940494 31.14516259878841 22.567244036849562 17.89407354442153 8 24.696419860755938 37.855870571018905 20.425788236685342 17.021921331539815 9 26.13974159746292 15.643616264865171 20.05386670047613 38.162775437195776 10 13.61507652298471 29.951569051476568 35.23481882722702 21.198535598311697 11 33.27598485564171 22.878772464010346 24.38666802316709 19.45857465718085 12 22.595183640521995 25.294613865850245 32.01464615429367 20.095556339334088 13 27.821920771148847 21.660810408224066 25.531450352395762 24.985818468231326 14 20.28242519405417 22.136885001906162 28.30466548177136 29.276024322268313 15 22.477235763752915 30.871162007980274 23.822451305173065 22.82915092309375 16 21.848426288698036 28.777949317628604 27.226671659771707 22.146952733901653 17 21.326895539864708 28.40735149929109 28.613499615566383 21.652253345277813 18 21.02908666514766 28.040115699633176 29.905363544179625 21.025434091039543 19 22.324714586966007 27.523777900243214 27.730829475382418 22.42067803740836 20 22.502831840181237 28.011018432705182 28.31582741221295 21.170322314900627 21 22.01992331673057 26.650661124449066 27.03300247249794 24.296413086322428 22 21.8833006673606 27.96213068013688 28.516523876331153 21.638044776171363 23 21.960291814727118 27.789561695376403 28.201104071219792 22.04904241867669 24 21.86941125806518 27.756454497415188 28.318173013816356 22.055961230703268 25 22.26718913575001 27.525405259072816 28.18009077529089 22.027314829886283 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 676.0 1 676.0 2 1360.5 3 2045.0 4 2045.0 5 2045.0 6 3014.0 7 3983.0 8 3983.0 9 3983.0 10 4620.0 11 5257.0 12 5257.0 13 5257.0 14 6476.0 15 7695.0 16 7695.0 17 7695.0 18 10564.0 19 13433.0 20 13433.0 21 13433.0 22 18450.0 23 23467.0 24 23467.0 25 23467.0 26 30033.0 27 36599.0 28 36599.0 29 36599.0 30 43750.0 31 50901.0 32 50901.0 33 50901.0 34 56783.5 35 62666.0 36 62666.0 37 62666.0 38 66068.0 39 69470.0 40 69470.0 41 69470.0 42 70703.5 43 71937.0 44 71937.0 45 71937.0 46 71160.5 47 70384.0 48 70384.0 49 70384.0 50 65081.5 51 59779.0 52 59779.0 53 59779.0 54 51979.0 55 44179.0 56 44179.0 57 44179.0 58 36740.0 59 29301.0 60 29301.0 61 29301.0 62 23266.5 63 17232.0 64 17232.0 65 17232.0 66 13109.5 67 8987.0 68 8987.0 69 8987.0 70 6502.5 71 4018.0 72 4018.0 73 4018.0 74 2760.5 75 1503.0 76 1503.0 77 1503.0 78 1031.5 79 560.0 80 560.0 81 560.0 82 365.5 83 171.0 84 171.0 85 171.0 86 107.5 87 44.0 88 44.0 89 44.0 90 27.5 91 11.0 92 11.0 93 11.0 94 5.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.237765660673151 2 36.30955437122838 3 5.772910398461059 4 13.80288825222746 5 13.445022916388554 6 14.696952582415138 7 9.284816994068096 8 7.374661559683586 9 0.10867742145275186 10 1.548182605451328 11 5.795330464933989 12 5.862761809898374 13 16.126017888132427 14 1.6849278963816408 15 2.9779324933509956 16 3.112110601097385 17 2.948837750599865 18 1.6020934523137167 19 1.553830408455959 20 0.2803364036844213 21 4.476825181670996 22 1.6765417646474916 23 0.10953314918072628 24 8.991473528918464 25 0.11278491454702907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 584298.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.84537318276959 #Duplication Level Percentage of deduplicated Percentage of total 1 97.74568016497717 93.68471192415876 2 1.7735827733074387 3.399794055563658 3 0.24914175149695394 0.7163725244290318 4 0.08408482394445993 0.32236565319856963 5 0.03815222178158105 0.18283569672037125 6 0.02498056704453335 0.14365630624202966 7 0.018991553968955965 0.12741768042327056 8 0.011015426784015233 0.08446221527051323 9 0.008107375869185758 0.06993490191435808 >10 0.04101491941774741 0.7074792274777689 >50 0.002714727349916887 0.154208036640418 >100 0.0025336940580268485 0.4067617779612478 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.711455455948848E-4 0.0 0.0 0.0 6 0.0 1.711455455948848E-4 0.0 0.0 0.0 7 0.0 1.711455455948848E-4 0.0 0.0 0.0 8 0.0 1.711455455948848E-4 0.0 0.0 0.0 9 0.0 1.711455455948848E-4 0.0 1.711455455948848E-4 0.0 10 0.0 1.711455455948848E-4 0.0 1.711455455948848E-4 0.0 11 0.0 1.711455455948848E-4 0.0 1.711455455948848E-4 0.0 12 0.0 1.711455455948848E-4 0.0 1.711455455948848E-4 0.0 13 0.0 1.711455455948848E-4 0.0 1.711455455948848E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTATA 60 1.0177264E-6 19.995752 1 AGGTGTA 35 0.0013753292 17.430733 7 GTCCTAC 165 0.0 16.158184 1 GCTATAT 50 0.002440469 15.996602 1 ACACCGT 40 0.002904328 15.586097 6 TAACTCA 50 6.0959073E-4 14.976994 4 TATTGTA 65 7.0475106E-4 14.691632 2 AGTAAGC 65 7.0475106E-4 14.691632 2 GTAGGAC 175 0.0 13.721876 3 TATAGGA 100 6.842485E-6 13.64223 2 GTCTGGA 70 0.0014696388 13.3305025 1 GTCCTAT 70 0.0014696388 13.3305025 1 CCTCTAG 60 0.0081927935 13.330502 1 TAGGACC 105 5.7461875E-8 13.244961 4 TGTAGGA 165 3.6743586E-10 13.22883 2 CTGCACT 65 2.942757E-4 13.166587 4 GTAACCC 50 0.0092064515 13.098153 3 TGGACAG 90 2.721641E-6 12.70276 5 AGGACGT 215 0.0 12.5684805 5 TCCTACA 250 0.0 12.550853 2 >>END_MODULE