FastQCFastQC Report
Thu 26 May 2016
SRR1926592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584298
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11530.19733081407090217No Hit
GGTATCAACGCAGAGTACTTTTTTT6780.11603667991333189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG250.00603142718.9979429
GTACTAG500.001455733613.3501031
TAGGACG2750.013.1778614
GTAGGAC2900.013.1550783
GGACGTG2800.012.9414266
AGGACGT2850.012.715485
GGTTAAG603.9576137E-412.7143841
TCCTATA701.04854604E-412.2634682
TAAGCTG550.002977441812.1375035
GATCTAG550.002979364212.1364581
GTCCTAC2800.011.9197351
CTAGGAC907.119348E-611.6568593
GTATAGC1001.8375122E-611.44294551
GACGTGA1501.7462298E-1011.40177
CGTGAAA1751.8189894E-1211.3987669
TGGCGAG2250.011.36949818
TAGGCAG1104.7071262E-711.2705385
GTCCTAG855.1097995E-511.2185751
TAGGACC1201.2032251E-711.1260444
TACACCG600.00570251411.1260445
Produced by FastQC (version 0.11.5)