FastQCFastQC Report
Thu 26 May 2016
SRR1926591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739985
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG552.4472535E-416.8852651
TCTAGGT607.1477716E-616.3072413
AAAGGCG450.006489090613.8108845
ATATGAC807.14226E-613.5893683
GTCTAAA600.00846059313.2669941
TGTAGGA1757.2759576E-1113.1830592
GCTACAC755.8764017E-513.04579453
TGCTAGA853.8041666E-412.7725122
GTCCTAA1051.5107396E-512.6352331
TAGGACC1202.5547706E-812.4385114
GTATTAA750.00256124512.3825291
TTCTAGG1255.4265474E-611.94232
CCTCTAA907.447516E-411.7928851
CTGTAGG1701.22690835E-811.7061711
TATATGA1401.5049663E-611.632112
GTATTAG800.004151864411.6086211
GTATCAA3200.011.6086211
GTCTTAG800.004151864411.6086211
TCTATAC851.8906727E-411.5109943
CCTAGGA1204.7009948E-511.3089952
Produced by FastQC (version 0.11.5)