FastQCFastQC Report
Thu 26 May 2016
SRR1926591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739985
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11620.15703021007182577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGCG508.3797255E-515.2678015
AAGGCGT500.001449558413.35841756
TAGGACC1206.87578E-1012.7231674
AGTAGGA802.7246051E-511.9320312
GTCCTAC2200.011.7087061
GTAGGAC2400.011.5327253
CCCTATA751.9808293E-411.454752
GTATTAT751.9908801E-411.4485131
GCATTAT600.00568798511.1304981
ATAGTAC700.001433029210.90783
GTATTAG700.00143792310.9033451
GAGTTAA700.00143792310.9033451
TGTAGGA2900.010.8622632
TATACTA1002.2789076E-510.49661355
GACAGTA1002.3963295E-510.4489577
TTATACA1752.1645974E-1010.3602934
GGACGTG1752.1645974E-1010.3595886
CTTCTAG1201.438555E-610.3354631
GTGCATT2300.010.30132111
CTGTAGG2800.010.2218871
Produced by FastQC (version 0.11.5)