##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926591_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739985 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.55316932099975 31.0 28.0 31.0 23.0 34.0 2 29.000029730332372 31.0 28.0 33.0 23.0 34.0 3 29.355065305377813 31.0 30.0 33.0 25.0 34.0 4 33.14367183118577 35.0 33.0 37.0 28.0 37.0 5 32.870193314729356 35.0 33.0 37.0 26.0 37.0 6 32.940671770373726 35.0 33.0 37.0 27.0 37.0 7 32.797120211896186 35.0 33.0 37.0 26.0 37.0 8 32.755150442238694 35.0 33.0 37.0 26.0 37.0 9 34.03762508699501 38.0 34.0 39.0 25.0 39.0 10 33.95377473867713 37.0 34.0 39.0 25.0 39.0 11 34.174407589343026 38.0 34.0 39.0 26.0 39.0 12 33.97824820773394 37.0 34.0 39.0 25.0 39.0 13 34.11325634979087 38.0 34.0 39.0 26.0 39.0 14 34.81734224342385 39.0 34.0 40.0 25.0 41.0 15 34.922148421927474 39.0 34.0 40.0 25.0 41.0 16 34.899673642033285 39.0 34.0 40.0 25.0 41.0 17 34.8110677919147 39.0 34.0 40.0 25.0 41.0 18 34.77798198612134 39.0 34.0 40.0 25.0 41.0 19 34.82562349236809 39.0 34.0 40.0 25.0 41.0 20 34.82178557673466 39.0 34.0 40.0 25.0 41.0 21 34.744542119097005 39.0 34.0 40.0 24.0 41.0 22 34.66945005642006 39.0 34.0 40.0 24.0 41.0 23 34.341681250295615 39.0 34.0 40.0 18.0 41.0 24 34.41557058589026 39.0 34.0 41.0 19.0 41.0 25 34.21911119819996 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6205221019178246 1101 2 0.5502321168216078 1101 3 0.49710057299882493 1101 4 0.36110644216051924 1101 5 0.491004846290096 1101 6 0.23503722552018758 1101 7 0.480798308878839 1101 8 0.4955361705507073 1101 9 0.9066220891025765 1101 10 0.7876964852828863 1101 11 0.5005706304847237 1101 12 0.5783733270484674 1101 13 0.48225084993436695 1101 14 0.8759239662255283 1101 15 0.7657040821902186 1101 16 0.7458511601736859 1101 17 0.6694607239990304 1101 18 0.8729440749302526 1101 19 0.8983061327742305 1101 20 0.8867665168020409 1101 21 0.8167333520517204 1101 22 0.6571990900610771 1101 23 1.071351017565739 1101 24 0.8526925452411547 1101 25 0.9727960454788516 1102 1 0.9166241286403292 1102 2 0.9163971590901241 1102 3 0.8399594128186898 1102 4 0.6900229031813652 1102 5 0.9412125495358978 1102 6 0.6578574477923596 1102 7 0.85197280173238 1102 8 0.8781090122595216 1102 9 1.1943024392225894 1102 10 1.1731376930944393 1102 11 0.9432394104799897 1102 12 1.1039254263826734 1102 13 0.9754397905954022 1102 14 1.2620004536590983 1102 15 1.010464335463574 1102 16 0.9438563472230683 1102 17 1.0044283544351487 1102 18 0.916375423388402 1102 19 0.8953690563182377 1102 20 1.0262887119930113 1102 21 1.0357982623730635 1102 22 1.0122918665067004 1102 23 1.139327683995603 1102 24 1.0170360643991216 1102 25 1.0600168514396486 1103 1 0.1444837337431757 1103 2 0.08211705849099005 1103 3 -0.027152076765702304 1103 4 -0.18930895130266379 1103 5 0.15099888735134215 1103 6 -0.043869979021536665 1103 7 0.04069488202711824 1103 8 0.24261732991074325 1103 9 0.4866520223763189 1103 10 0.5665863673282345 1103 11 0.1560850739822044 1103 12 0.14367913839954838 1103 13 0.1535091329747189 1103 14 0.4567068506752392 1103 15 0.3824311466275958 1103 16 0.47882949781455153 1103 17 0.5503258325648872 1103 18 0.5023385035534815 1103 19 0.4252818789696349 1103 20 0.3672429085491373 1103 21 0.37740135370544436 1103 22 0.26200596732310544 1103 23 0.6694887337216215 1103 24 0.36302021758267244 1103 25 0.4029124757511795 1104 1 0.7430654412289925 1104 2 0.7942105966119257 1104 3 0.684550566101322 1104 4 0.5558965281493897 1104 5 0.6132653879740033 1104 6 0.19923573565952069 1104 7 0.24871342360730608 1104 8 0.43164804805105206 1104 9 0.8069405238292049 1104 10 0.857218718234563 1104 11 0.5746820021681742 1104 12 0.761777934580536 1104 13 0.4731472954211 1104 14 0.9425343032835087 1104 15 0.7769240205725296 1104 16 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0.8995234740983022 2202 24 0.6627239367781854 2202 25 0.9089380139946428 2203 1 0.0015183917872079178 2203 2 0.19963324413983585 2203 3 -0.21413364442499372 2203 4 -0.3772773155507707 2203 5 -0.06424657374848408 2203 6 -0.2799109948029965 2203 7 0.18754914928513244 2203 8 0.18982189175121533 2203 9 0.49876668012904446 2203 10 0.5352091488565733 2203 11 0.37260863693690993 2203 12 0.2872962758883517 2203 13 0.1932031167927093 2203 14 0.530931432470112 2203 15 0.44757792806854013 2203 16 0.4078420457888825 2203 17 0.5508865952020727 2203 18 0.4976557714013907 2203 19 0.6352583560694001 2203 20 0.5889480639240858 2203 21 0.7134511086448896 2203 22 0.48292459847966285 2203 23 0.6249772890659813 2203 24 0.6526359131104087 2203 25 0.6201388126602865 2204 1 0.20049382844461405 2204 2 0.37770051095290214 2204 3 0.17078295073796212 2204 4 0.024386903022744377 2204 5 0.09937573153115409 2204 6 0.01334875030445204 2204 7 0.387600777388279 2204 8 0.23363063992726296 2204 9 0.6137494949998796 2204 10 0.44364682913075626 2204 11 0.20890876830983274 2204 12 0.3700951492807292 2204 13 0.20034864017563336 2204 14 0.6799318348299792 2204 15 0.5272463932353233 2204 16 0.6607955126281269 2204 17 0.3265491558590341 2204 18 0.36383475852102976 2204 19 0.4144330820526321 2204 20 0.5115180284873304 2204 21 0.4458054471022521 2204 22 0.45534355690086414 2204 23 0.6180656676526795 2204 24 0.6173470138797441 2204 25 0.49565812350994776 2205 1 0.5518357726051164 2205 2 0.6669716143618061 2205 3 0.6450537043884275 2205 4 0.4199648280751518 2205 5 0.42498165982390645 2205 6 0.1892016531866858 2205 7 0.25726103783420484 2205 8 0.1066581715578323 2205 9 0.6171602039263959 2205 10 0.6289403892880827 2205 11 0.4745807671003419 2205 12 0.6049985595383589 2205 13 0.2834817085951542 2205 14 0.7255318736418843 2205 15 0.667522657273615 2205 16 0.49606041515834676 2205 17 0.5747603106711452 2205 18 0.5925609834089371 2205 19 0.6379242128555234 2205 20 0.5935160743530759 2205 21 0.5261218318184859 2205 22 0.796625432869746 2205 23 1.1649598126963738 2205 24 0.7315815054776422 2205 25 0.8018575354644639 2206 1 0.1997248496848094 2206 2 0.4263281796277134 2206 3 0.6549642841198313 2206 4 0.403850619965759 2206 5 0.7286210403057041 2206 6 0.4069521210705176 2206 7 0.5592650476319818 2206 8 0.7268304105986871 2206 9 0.9491243380190113 2206 10 0.8774600537453594 2206 11 0.6835370194064225 2206 12 0.8185280706719951 2206 13 0.5435156496217886 2206 14 0.8088063047351497 2206 15 0.8055939534340766 2206 16 0.6510899996419468 2206 17 0.783565973288006 2206 18 0.716425170402438 2206 19 0.5539890764468254 2206 20 0.7598526662027822 2206 21 0.8334025932355118 2206 22 0.8546369154724687 2206 23 1.1766741346093426 2206 24 0.9518186481188593 2206 25 0.9909259465265237 2207 1 -0.68429682300971 2207 2 0.32315080452373834 2207 3 0.3564597965485419 2207 4 0.5380658809250392 2207 5 0.6457405618152592 2207 6 0.6578574477923596 2207 7 0.6071531547638429 2207 8 0.7929209846309746 2207 9 0.9341335980867456 2207 10 0.7372205787429422 2207 11 0.6232087120916603 2207 12 0.8238026328293344 2207 13 0.6853400208333156 2207 14 -4.496249738205826 2207 15 -1.4576860866392707 2207 16 -0.26105539491047125 2207 17 -0.12757471540368215 2207 18 0.2402434203185635 2207 19 0.2883084270012759 2207 20 0.6640477296445866 2207 21 0.6697199814828139 2207 22 0.7444484282872068 2207 23 0.9996500170731153 2207 24 0.7108196407613576 2207 25 0.9218741039338951 2208 1 0.5548424004010322 2208 2 0.8220490389221489 2208 3 0.7213319106612488 2208 4 0.5745168851199622 2208 5 0.7010520827071502 2208 6 0.5076458132808881 2208 7 0.6803167214189969 2208 8 0.8872624408940979 2208 9 1.1507976917457086 2208 10 1.0414006241241367 2208 11 0.6499659560338884 2208 12 0.7194808684050358 2208 13 0.6338180404985039 2208 14 1.0550995359283348 2208 15 0.7193438776088783 2208 16 0.7558825852377566 2208 17 0.7850120680738328 2208 18 0.7981333170613638 2208 19 0.6000875452111742 2208 20 0.3936148090661149 2208 21 0.8272369216842606 2208 22 0.7610632090502918 2208 23 0.9821705946559618 2208 24 0.8222084762773036 2208 25 0.8448887878482267 2209 1 -0.40103243131578736 2209 2 -0.38038960508811925 2209 3 0.24024499053584236 2209 4 0.2720085196971951 2209 5 0.2717103766884037 2209 6 0.33664028831763915 2209 7 0.6130149171480994 2209 8 0.639993387339338 2209 9 0.9884213383131879 2209 10 0.5812450352878216 2209 11 0.6137589843513354 2209 12 0.48753791152910253 2209 13 0.6735879139679 2209 14 0.6826449981881808 2209 15 0.7374244287749931 2209 16 0.7806130454678808 2209 17 0.7925942716832921 2209 18 0.7305826715649246 2209 19 0.7907354727192555 2209 20 0.5744398152011385 2209 21 0.6654247612607946 2209 22 0.5510400679630507 2209 23 0.6611619735115681 2209 24 0.6878651762986365 2209 25 0.7298656582283201 2210 1 -0.3956107948156635 2210 2 -0.3908642606191286 2210 3 0.3983065469625693 2210 4 0.5380131099264105 2210 5 0.845101576686254 2210 6 0.9337279412468007 2210 7 1.285249411671309 2210 8 1.078950872561009 2210 9 1.4707788219204758 2210 10 1.4482615746424443 2210 11 1.1789316291682255 2210 12 1.2204906988960218 2210 13 1.127555125847607 2210 14 1.4584408217791633 2210 15 1.0206087455815336 2210 16 1.1343687731645105 2210 17 1.1015374158019569 2210 18 1.1605924385825404 2210 19 1.2850757147152763 2210 20 1.4938202149762034 2210 21 1.6529993928451248 2210 22 1.4127748840175514 2210 23 1.7770840956719383 2210 24 1.4375564255351705 2210 25 1.5509408911703488 2211 1 -1.05670938512014 2211 2 -0.11708625095696235 2211 3 0.3345830063730588 2211 4 0.4817571494760031 2211 5 0.9302335583426782 2211 6 0.6308230190729986 2211 7 1.0167807467124135 2211 8 0.8230780915591822 2211 9 1.0328259524224421 2211 10 0.9910100318747439 2211 11 0.7637820708652185 2211 12 0.8928366323898658 2211 13 0.7035438300367787 2211 14 0.8433643925896703 2211 15 0.667924707542987 2211 16 0.7825763971240605 2211 17 0.8342398145224124 2211 18 1.0914754476816313 2211 19 0.9097793849984868 2211 20 1.0290711561749788 2211 21 1.107697831474404 2211 22 0.9634463746397799 2211 23 1.2604832166970539 2211 24 1.1080370677028668 2211 25 1.0714462660889126 2212 1 1.0785326790522518 2212 2 0.233096841257332 2212 3 0.7612164394949446 2212 4 0.7393171215176295 2212 5 0.6384597398699299 2212 6 0.750032975069324 2212 7 0.9190846753921704 2212 8 0.8433369263629658 2212 9 0.8497733018714086 2212 10 0.8242026941190588 2212 11 0.6793464476703122 2212 12 0.6690443156689128 2212 13 0.754009583220558 2212 14 0.7183275763280932 2212 15 0.8986121056113987 2212 16 0.40356672159071394 2212 17 0.8907873943202844 2212 18 0.927956296631649 2212 19 0.9787455843733781 2212 20 0.8779332589439974 2212 21 0.8384291892581288 2212 22 1.0889601538125433 2212 23 1.470109515157148 2212 24 1.396788655747585 2212 25 1.0665469558168468 2213 1 0.12868809108521972 2213 2 0.07055432587536359 2213 3 -0.3908309866524924 2213 4 -1.5737871701474475 2213 5 -2.057913554737784 2213 6 -0.9450409730332474 2213 7 -0.5538026126678588 2213 8 -0.3950772339661981 2213 9 -1.1788314292785103 2213 10 -1.0329217374168422 2213 11 -0.8313620146422664 2213 12 -1.0588922711261617 2213 13 -1.195803158018407 2213 14 -8.433151385723345 2213 15 -4.151216877458882 2213 16 -2.6041385942651303 2213 17 -1.9283040822214161 2213 18 -6.22527901247269 2213 19 -3.9039199230723867 2213 20 -5.720713573580426 2213 21 -2.8846030014146002 2213 22 -2.416020674457158 2213 23 -4.957034725043613 2213 24 -2.8510792058083183 2213 25 -2.374283415770776 2214 1 0.3191880948594026 2214 2 0.2383865860826404 2214 3 -1.6550870196841423 2214 4 -2.315285822764089 2214 5 -0.3299642193714263 2214 6 -0.2755386368139199 2214 7 -0.1864358979432552 2214 8 -0.14528858821856971 2214 9 -5.293759078188906 2214 10 -1.3353857128393614 2214 11 -0.637113303525787 2214 12 -0.7432516790323191 2214 13 -0.9071706200312519 2214 14 -2.2010674327949573 2214 15 -1.4796019422616027 2214 16 -0.9623000764623129 2214 17 -1.421718132750179 2214 18 -0.7592891197349729 2214 19 -3.7566597912779116 2214 20 -1.4380942527824132 2214 21 -3.1059833711335756 2214 22 -5.47222203467274 2214 23 -5.991087291865526 2214 24 -5.151374913052486 2214 25 -3.049768775714618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21876.0 3 12.0 4 389.0 5 2642.0 6 3207.0 7 2672.0 8 1944.0 9 1723.0 10 1492.0 11 1381.0 12 1357.0 13 1289.0 14 1301.0 15 1355.0 16 1307.0 17 1396.0 18 1639.0 19 1956.0 20 2400.0 21 3301.0 22 4252.0 23 5132.0 24 6306.0 25 7408.0 26 8636.0 27 9582.0 28 11214.0 29 13583.0 30 16561.0 31 20984.0 32 27208.0 33 35214.0 34 46722.0 35 61657.0 36 84064.0 37 124599.0 38 202224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.828943506424125 18.974927061649645 9.05734029646041 30.138789135465828 2 14.315780082886596 21.275847992581838 37.879254831672085 26.52911709285948 3 18.418726243450898 23.986342180817413 28.83727405933528 28.757657516396414 4 12.222132100467732 17.058425933436073 36.6668018492984 34.0526401167978 5 13.387993652698821 37.95513096039424 35.098439937175776 13.55843544973116 6 32.58689154887349 37.81374771570092 16.881902924581162 12.717457810844435 7 28.774496329866533 31.663753217544606 21.035192930812467 18.526557521776397 8 25.853754978177946 37.21612029146631 19.397730181062816 17.53239454929293 9 25.815900374840204 15.800300088834963 19.457971312807402 38.925828223517435 10 13.999189079605351 30.40032436815786 33.919854034328964 21.680632517907824 11 35.229393504898155 23.157196276080345 22.70866639728747 18.904743821734034 12 22.245166312369673 25.461273063653184 31.3196740918127 20.973886532164446 13 28.475712345520517 21.890849138833897 25.461597194537728 24.17184132110786 14 20.674291308557304 21.798245376805873 27.54039040906952 29.987072905567302 15 23.010746294474416 30.32260505746132 23.08237023903478 23.584278409029483 16 22.59510520696785 29.018473721907647 26.27701123018501 22.1094098409395 17 21.938934784577064 28.48858659149174 27.223117912652388 22.349360711278806 18 21.635559423595918 28.611816472896763 28.053548333912186 21.69907576959513 19 22.87752964627183 27.687692151761883 27.274299807425926 22.160478394540355 20 22.82087527163043 27.79265814026401 27.724682962690682 21.66178362541488 21 22.84373716188458 27.130721745335034 27.282346645332872 22.74319444744751 22 22.366264181030697 27.759346473239322 27.472854179476613 22.40153516625337 23 22.522888977479276 27.479070521699768 27.20798394562052 22.790056555200444 24 22.196786133698026 27.562464441654583 27.894364855313853 22.346384569333537 25 22.56059244444144 27.47528666121611 27.467043250876706 22.497077643465747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 162.0 3 155.0 4 155.0 5 155.0 6 307.5 7 460.0 8 460.0 9 460.0 10 827.5 11 1195.0 12 1195.0 13 1195.0 14 1979.5 15 2764.0 16 2764.0 17 2764.0 18 4931.0 19 7098.0 20 7098.0 21 7098.0 22 11483.0 23 15868.0 24 15868.0 25 15868.0 26 22716.5 27 29565.0 28 29565.0 29 29565.0 30 38776.5 31 47988.0 32 47988.0 33 47988.0 34 57322.5 35 66657.0 36 66657.0 37 66657.0 38 74683.5 39 82710.0 40 82710.0 41 82710.0 42 88797.5 43 94885.0 44 94885.0 45 94885.0 46 97924.0 47 100963.0 48 100963.0 49 100963.0 50 98360.0 51 95757.0 52 95757.0 53 95757.0 54 85802.0 55 75847.0 56 75847.0 57 75847.0 58 64874.5 59 53902.0 60 53902.0 61 53902.0 62 43758.5 63 33615.0 64 33615.0 65 33615.0 66 25789.5 67 17964.0 68 17964.0 69 17964.0 70 12859.0 71 7754.0 72 7754.0 73 7754.0 74 5370.5 75 2987.0 76 2987.0 77 2987.0 78 2093.0 79 1199.0 80 1199.0 81 1199.0 82 771.0 83 343.0 84 343.0 85 343.0 86 230.0 87 117.0 88 117.0 89 117.0 90 67.5 91 18.0 92 18.0 93 18.0 94 11.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08946127286363913 2 0.024189679520530822 3 0.025541058264694553 4 0.033108779232011464 5 0.019324716041541384 6 0.3902781813144861 7 0.19676074515023956 8 0.20432846611755642 9 0.20770691297796579 10 0.011486719325391732 11 0.0016216544929964797 12 0.0029730332371602124 13 0.0 14 0.06121745711061711 15 0.0012162408697473598 16 0.0020270681162456 17 0.0029730332371602124 18 0.0024324817394947196 19 0.001351378744163733 20 0.0012162408697473598 21 1.351378744163733E-4 22 0.0 23 0.0 24 5.405514976654932E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 739985.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.59056674485112 #Duplication Level Percentage of deduplicated Percentage of total 1 93.2564334536725 82.61640292264339 2 5.280690691682809 9.356387623608796 3 0.7752826875186816 2.0604819802425394 4 0.26066247018781025 0.9236894385220388 5 0.1231694624045598 0.5455826240039293 6 0.07724654433481647 0.4105989085021596 7 0.04798767671416778 0.2975878833813773 8 0.03665487499277051 0.25978209196569674 9 0.02477806076950429 0.19755922017678723 >10 0.10531234874068246 1.618203924345021 >50 0.005967675619942151 0.3708809520919259 >100 0.005202047755079521 0.9291596738832819 >500 4.590042049778714E-4 0.2561796887407345 >1k 1.5300140165929048E-4 0.15750306789245158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1162 0.15703021007182577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.351378744163733E-4 0.0 4 0.0 0.0 0.0 1.351378744163733E-4 0.0 5 0.0 0.0 0.0 1.351378744163733E-4 0.0 6 0.0 0.0 0.0 1.351378744163733E-4 0.0 7 0.0 0.0 0.0 1.351378744163733E-4 0.0 8 0.0 0.0 0.0 1.351378744163733E-4 0.0 9 0.0 0.0 0.0 1.351378744163733E-4 0.0 10 0.0 0.0 0.0 2.702757488327466E-4 0.0 11 1.351378744163733E-4 0.0 0.0 2.702757488327466E-4 0.0 12 1.351378744163733E-4 0.0 0.0 4.0541362324911993E-4 0.0 13 1.351378744163733E-4 0.0 0.0 4.0541362324911993E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAGGCG 50 8.3797255E-5 15.267801 5 AAGGCGT 50 0.0014495584 13.3584175 6 TAGGACC 120 6.87578E-10 12.723167 4 AGTAGGA 80 2.7246051E-5 11.932031 2 GTCCTAC 220 0.0 11.708706 1 GTAGGAC 240 0.0 11.532725 3 CCCTATA 75 1.9808293E-4 11.45475 2 GTATTAT 75 1.9908801E-4 11.448513 1 GCATTAT 60 0.005687985 11.130498 1 ATAGTAC 70 0.0014330292 10.9078 3 GTATTAG 70 0.001437923 10.903345 1 GAGTTAA 70 0.001437923 10.903345 1 TGTAGGA 290 0.0 10.862263 2 TATACTA 100 2.2789076E-5 10.4966135 5 GACAGTA 100 2.3963295E-5 10.448957 7 TTATACA 175 2.1645974E-10 10.360293 4 GGACGTG 175 2.1645974E-10 10.359588 6 CTTCTAG 120 1.438555E-6 10.335463 1 GTGCATT 230 0.0 10.301321 11 CTGTAGG 280 0.0 10.221887 1 >>END_MODULE