FastQCFastQC Report
Thu 26 May 2016
SRR1926590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860521
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA3650.014.6079782
GTATTAA658.305537E-414.3788961
GTCCTAA1901.8189894E-1213.3518311
GTGCAAG802.6161637E-413.35183051
GTTTAAG700.001453731313.35183051
CAATTAT700.001453731313.35183051
CCACTAA600.00811378713.35183051
CTGTAGG4050.013.1869931
TCAGGAC1252.235538E-913.1293953
ACCGTCC450.00972936712.9907188
CTAGACA756.724417E-512.8602344
CAGGACC1253.0468073E-912.8602344
AGGACGT3500.012.7874185
TCTAGGA1401.0014264E-712.6950262
GTCCTAC3900.012.6671211
TAGGACG3900.012.6404014
TTAGGAC2350.012.5706983
CCTTATA905.788313E-412.1525062
GGACGTG3550.012.0142026
GTAGGAC4100.012.0085933
Produced by FastQC (version 0.11.5)