FastQCFastQC Report
Thu 26 May 2016
SRR1926590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860521
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13630.15839241575743068No Hit
GTCCTACAGTGTGCATTTCTCATTT10460.12155426770526227No Hit
GTCCTACAGTGGACATTTCTAAATT10070.11702212961682515No Hit
CTGTAGGACGTGGAATATGGCAAGA9660.11225757419051946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.012.9405534
GCTATAC657.772397E-411.7355613
GTCCTAC7200.011.384541
GTCCTAA3100.011.3759991
TCCTACA7900.011.2262292
ATTTCTC7700.011.19743515
TTAGGAC3000.011.1243353
TAGAGTG1201.2087185E-711.1243345
TTACACC600.00571278911.1243344
GCATTTC5400.011.06287913
AGGACGT5050.010.9512565
GGCAGCG700.001488585410.8585757
TGTAGGA7950.010.7957652
GTGTATT3700.010.76388211
GGACGTG5100.010.655686
GTAGGAC7200.010.59460453
GTCTTAC909.191556E-510.5902691
GGCGAGA3950.010.55415419
CAGTACT1002.299272E-510.4886594
GATATAC2001.8189894E-1210.4843671
Produced by FastQC (version 0.11.5)