FastQCFastQC Report
Thu 26 May 2016
SRR1926589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591492
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG859.175346E-814.6441885
TCTAGAC450.00466613614.5138643
GAGTAAG550.004680504614.5060571
GTCTTAT550.004680504614.5060571
TGTAGAA1704.3655746E-1113.5989272
CTAGACT550.00127458513.5860084
TAGGACC952.5136978E-713.4838584
GATATAC1008.916308E-613.2972191
CCTATAC500.00937406413.0624773
GTCCTAC2300.012.7190791
ATAGGAC704.7502384E-412.44045453
CTACACT953.6957554E-612.3602034
CTAGACA705.600306E-412.1996824
GGACAGT852.166641E-512.1992666
TGTAGGA2251.8189894E-1212.08793452
AGGACTG707.127495E-411.8548195
CTATATA907.299704E-411.819751
GCTACAC650.003858907611.7227353
GTAGGAC2800.011.6629273
TTAATAC851.8665583E-411.5257153
Produced by FastQC (version 0.11.5)