FastQCFastQC Report
Thu 26 May 2016
SRR1926589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591492
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9770.1651755222386778No Hit
CTGTAGGACGTGGAATATGGCAAGA9180.15520074658659797No Hit
GTCCTACAGTGGACATTTCTAAATT8220.13897060315270537No Hit
CTTTAGGACGTGAAATATGGCGAGG6920.11699228391930915No Hit
GTCCTACAGTGTGCATTTCTCATTT6700.11327287604904208No Hit
GTCCTAAAGTGTGTATTTCTCATTT6380.10786282823774455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC400.00510889514.3184433
ATAAGAC801.2107193E-714.3184433
GCTCTAA551.8884652E-413.8762751
GATATAC1500.013.3559141
TAGGACC1800.013.2566874
GTATTAG701.0468572E-412.26563551
AGGACGT4750.012.2575535
TATAGTG802.7247816E-511.931025
GTAGGAC5000.011.8365793
GGACGTG4700.011.7787086
GTCCTAA1950.011.7414631
TGTAGGA5650.011.4905022
ACTACAC751.9795274E-411.4547553
CTGTAGG5350.011.4122631
ATGGGTT855.060987E-511.2291965
TAGGACG5050.011.1513684
TACTAAA600.00565545911.1384652
ACTGTAC700.001430653510.909293
GTCCTAT700.001437792210.9027871
GTGCTAG700.001437792210.9027871
Produced by FastQC (version 0.11.5)