FastQCFastQC Report
Thu 26 May 2016
SRR1926588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740722
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC607.41023E-616.2455483
TCTAGGA952.6239104E-715.6292542
GTATTAA604.9251114E-415.4038951
GTCCTAC2350.015.1697931
GCCTAGC550.004908890514.4036411
GTAGGAC2650.014.30425553
TAGGACC1500.014.1825014
TGTAGGA2600.014.0171462
TAATACT851.1050743E-613.7653684
TACCCTC450.006645879713.7612265
GACTTTA450.007614345813.4812787
GGACGTG2000.013.4123526
GGACTGA550.001648864513.1309746
ATAAGAC500.00969762212.9964373
CTTAGGC500.00969762212.9964373
TAGGACG2050.012.97184
CTGTAGG2750.012.9632781
TAAGAGT757.210155E-512.7642514
CCCTAAC750.002249967812.5981262
TCCTACA2800.012.533852
Produced by FastQC (version 0.11.5)