FastQCFastQC Report
Thu 26 May 2016
SRR1926588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740722
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11810.15943903380755534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC250.006036100418.996217
GCACCGT601.4073667E-615.8936296
TATGGAC400.00513496114.3081553
CCGTCTC400.00527550914.25005159
GTCTTAT759.2762093E-713.9825941
TTAGAGC550.002973695512.1402523
GCTATAC550.002973695512.1402523
TAGGACG3150.012.1094324
ACTGATC550.003065745612.0909528
ACCGATC951.037266E-612.0000428
GTAGGAC4150.011.9521933
AGGACGT3300.011.8479795
GTCCTAC4600.011.8133571
TACCGAT658.0281094E-411.6899767
CGGTTTC658.147386E-411.66944513
TAGGACC2550.011.5934
TGTAGGA4300.011.536042
GGACGTG3400.011.4995096
GGCGAGG1657.2759576E-1211.48487819
GTCCTAT752.0042014E-411.4403041
Produced by FastQC (version 0.11.5)