##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926588_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740722 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.576221578405935 31.0 28.0 31.0 23.0 34.0 2 29.034103212811285 31.0 28.0 33.0 24.0 34.0 3 29.39769576170277 31.0 30.0 33.0 25.0 34.0 4 33.25204462672906 35.0 33.0 37.0 28.0 37.0 5 33.023093684270215 35.0 33.0 37.0 27.0 37.0 6 33.14257845723497 35.0 33.0 37.0 28.0 37.0 7 32.980762013278934 35.0 33.0 37.0 26.0 37.0 8 32.96929077305656 35.0 33.0 37.0 26.0 37.0 9 34.26086304983516 38.0 34.0 39.0 26.0 39.0 10 34.15287516774174 37.0 34.0 39.0 26.0 39.0 11 34.40891184546969 38.0 35.0 39.0 27.0 39.0 12 34.21644152597061 38.0 34.0 39.0 26.0 39.0 13 34.354806526605124 38.0 35.0 39.0 27.0 39.0 14 35.047976163796946 39.0 34.0 40.0 25.0 41.0 15 35.169232181574195 39.0 34.0 40.0 27.0 41.0 16 35.14286601451017 39.0 34.0 40.0 26.0 41.0 17 35.05967285972335 39.0 34.0 40.0 26.0 41.0 18 35.029032484521856 39.0 34.0 40.0 26.0 41.0 19 35.060291175366736 39.0 34.0 40.0 25.0 41.0 20 35.03783065711563 39.0 34.0 41.0 25.0 41.0 21 34.973311174772725 39.0 34.0 41.0 25.0 41.0 22 34.89714764783549 39.0 34.0 40.0 25.0 41.0 23 34.564524882479525 39.0 34.0 40.0 22.0 41.0 24 34.63660455609527 39.0 34.0 41.0 24.0 41.0 25 34.438191386242075 39.0 34.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8236709978677119 1101 2 0.8383395014794708 1101 3 0.7363128966197081 1101 4 0.40277581991674793 1101 5 0.5935948891190606 1101 6 0.4124541946539253 1101 7 0.28630086435766344 1101 8 0.43058256237412706 1101 9 0.7665389653216792 1101 10 0.865115144981452 1101 11 0.5633083057834156 1101 12 0.7153725399880955 1101 13 0.6796733670379567 1101 14 1.0413776319897963 1101 15 0.8998554408972907 1101 16 1.0295436241926126 1101 17 0.8405826492202024 1101 18 0.9041237280621957 1101 19 0.9758958104528617 1101 20 1.1083161558623615 1101 21 1.1895188085696304 1101 22 0.9722294422746458 1101 23 1.2814160047073315 1101 24 1.2273196371149382 1101 25 1.1857421459907016 1102 1 0.8379957690536024 1102 2 0.8722031085525792 1102 3 0.8190527838016024 1102 4 0.6493999236891383 1102 5 0.748029129777855 1102 6 0.5035116909114947 1102 7 0.8546284091814371 1102 8 0.813529095492008 1102 9 1.247683625187534 1102 10 1.0508816901500069 1102 11 0.8788790109681841 1102 12 1.0712017508671892 1102 13 0.9031544478094204 1102 14 1.231488037758261 1102 15 1.0129852786537938 1102 16 0.898933699239457 1102 17 1.0905283991543868 1102 18 1.11358515984535 1102 19 0.9872497709279742 1102 20 1.0362368196848877 1102 21 1.0095336130546002 1102 22 1.2334217540764811 1102 23 1.6721448061498876 1102 24 1.3381665660104503 1102 25 1.484654501016351 1103 1 0.23560909661494733 1103 2 0.007831026903200922 1103 3 -0.11409240920195884 1103 4 -0.102751077651412 1103 5 -0.11605875863333637 1103 6 -0.34215155331954605 1103 7 0.09912326671580729 1103 8 0.1579222823446571 1103 9 0.37670845684710486 1103 10 0.4944445480765154 1103 11 0.2206406565571868 1103 12 0.12288912068079583 1103 13 0.14172200373656096 1103 14 0.6029104249153718 1103 15 0.4348591254956702 1103 16 0.5446504775455949 1103 17 0.3719017354398062 1103 18 0.44207509136359135 1103 19 0.4327860094211786 1103 20 0.42298085740988967 1103 21 0.37964408491450996 1103 22 0.2316251681405248 1103 23 0.48996427294609646 1103 24 0.35554366069636956 1103 25 0.481246935968592 1104 1 0.2932145946672229 1104 2 0.2507849349054396 1104 3 0.11634884888101382 1104 4 -0.05220491688518791 1104 5 0.19480039766052926 1104 6 -0.0975675383221315 1104 7 0.13121753677733494 1104 8 0.12294776413867226 1104 9 0.40884179381954766 1104 10 0.18002120511560094 1104 11 0.1398984494693778 1104 12 0.19115935024974107 1104 13 0.23823493408132634 1104 14 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1107 13 0.7623226836592707 1107 14 1.1828045339178033 1107 15 0.9417152432582014 1107 16 1.0931468104929252 1107 17 1.0819286282227765 1107 18 1.2380068268679167 1107 19 1.285284571204926 1107 20 0.9627181609644353 1107 21 1.143182724282184 1107 22 1.2983179938606142 1107 23 1.3452855667345958 1107 24 1.2429996502157366 1107 25 1.2942559608420297 1108 1 1.2211323669431984 1108 2 0.9895812029390534 1108 3 0.7851462528053794 1108 4 0.6327264424745778 1108 5 -0.6376460257101826 1108 6 0.23433704900767793 1108 7 0.6006225076721563 1108 8 0.7106754066054819 1108 9 1.1373466762656221 1108 10 1.1220089493388414 1108 11 0.9120215369604168 1108 12 0.8317080662355991 1108 13 0.7106179844378957 1108 14 1.1339585606833964 1108 15 0.9375809211105377 1108 16 1.0863869531029664 1108 17 1.0545586668276101 1108 18 1.1625246943064695 1108 19 1.1199999340893 1108 20 1.0856273331693842 1108 21 1.118528151443769 1108 22 1.1829431290417176 1108 23 1.3396408531580803 1108 24 1.361317745924488 1108 25 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0.5729069581185939 2204 11 0.4993894776484993 2204 12 0.41364788747448245 2204 13 0.3469465363243671 2204 14 0.7079008579832049 2204 15 0.6591621720453347 2204 16 0.6996760201149144 2204 17 0.5418295520412286 2204 18 0.5015479821616466 2204 19 0.7521847958371097 2204 20 0.6348816217876916 2204 21 0.7337719746652454 2204 22 0.7331597843725106 2204 23 1.0159018919900973 2204 24 0.8476470074404077 2204 25 0.7355466289368167 2205 1 0.860111273877628 2205 2 0.7921166837666007 2205 3 0.7336063916287507 2205 4 0.40171572746682926 2205 5 0.4844123340175628 2205 6 0.255821515615942 2205 7 0.231541033179262 2205 8 0.30247215840816466 2205 9 0.786906889217974 2205 10 0.9707610215809055 2205 11 0.7062669712888763 2205 12 0.8423853355933701 2205 13 0.82572816706476 2205 14 1.083191258304531 2205 15 0.8420327035756614 2205 16 0.7035191048652152 2205 17 0.7418766801708259 2205 18 0.8302607951218874 2205 19 0.7101561440764996 2205 20 0.8729461551924302 2205 21 0.8460456391450961 2205 22 0.7214521929948177 2205 23 1.1263418437908754 2205 24 1.0217067209228006 2205 25 1.0107754413615098 2206 1 0.33875124762229447 2206 2 0.3574097589224934 2206 3 0.4073838776806049 2206 4 0.40298386005625986 2206 5 0.7717927081560632 2206 6 0.4413206951367812 2206 7 0.6627848795855655 2206 8 0.5197822995305756 2206 9 0.8959479131759664 2206 10 0.8882746415349558 2206 11 0.46984294765908174 2206 12 0.6527168091739455 2206 13 0.345662528731971 2206 14 0.6636730436671812 2206 15 0.5763024771234129 2206 16 0.7076044346978421 2206 17 0.7324326368495306 2206 18 0.5427228889846987 2206 19 0.46659066983954034 2206 20 0.5091286297575763 2206 21 0.7206974277896663 2206 22 0.7250202927650804 2206 23 1.1428538227866056 2206 24 0.9344156149168938 2206 25 0.9552480373575705 2207 1 -0.7525337080517573 2207 2 0.08826569683304797 2207 3 0.17839371439772478 2207 4 0.4209300727545653 2207 5 0.4298663234532043 2207 6 0.45357408720337844 2207 7 0.38205535066605734 2207 8 0.51873404331225 2207 9 0.8621773924150844 2207 10 0.7207093118227732 2207 11 0.5650565181334741 2207 12 0.37619962527922013 2207 13 0.3129369521634473 2207 14 -4.821610520955964 2207 15 -1.7903498374307318 2207 16 -0.5950358020016182 2207 17 -0.6551141455172456 2207 18 0.02201003059589368 2207 19 0.38046640262528797 2207 20 0.3921699528015523 2207 21 0.5475507823199663 2207 22 0.5465208613295403 2207 23 0.6712020050363563 2207 24 0.6907570406336774 2207 25 0.5633492528710988 2208 1 0.4579752681510705 2208 2 0.8754724461144896 2208 3 0.874193363711786 2208 4 0.7764215256056985 2208 5 0.7601804084628014 2208 6 0.7534707640053497 2208 7 0.723595435469889 2208 8 0.6659252722234825 2208 9 1.0785661590819728 2208 10 1.0471132048307226 2208 11 0.8961137858916004 2208 12 0.7327790363807765 2208 13 0.5624783460196596 2208 14 1.1040416520053142 2208 15 0.9911522454511044 2208 16 0.8059156689310711 2208 17 0.8153369521822853 2208 18 0.892731804427541 2208 19 0.640189071778714 2208 20 0.7282417886927917 2208 21 1.0218967486654975 2208 22 0.884710603875277 2208 23 1.2544105624737654 2208 24 1.1422679234997517 2208 25 1.1151476675605068 2209 1 -0.22292213715578413 2209 2 0.2763497839997022 2209 3 0.47284493086729285 2209 4 0.6799938937449923 2209 5 0.6600207256259552 2209 6 0.7143576461520738 2209 7 0.7058223074605365 2209 8 0.7477122516482595 2209 9 0.8220335403446271 2209 10 0.6307019695218514 2209 11 0.7770797141016246 2209 12 0.6174280706361088 2209 13 0.7045471258837139 2209 14 0.9305012816145535 2209 15 0.9140762156191755 2209 16 0.9908602082063211 2209 17 1.1400926863868364 2209 18 1.0042405931016845 2209 19 0.9587777446980965 2209 20 0.8780805763268518 2209 21 0.8727309538304127 2209 22 0.7398764354190561 2209 23 0.9580061794552108 2209 24 0.9582288654449513 2209 25 1.0129261795122417 2210 1 -0.35333710824872 2210 2 -0.4080127461255394 2210 3 0.3814492267597238 2210 4 0.323962260594719 2210 5 0.7806619488095521 2210 6 0.5466104216251892 2210 7 0.4333900316384316 2210 8 0.6016216191169477 2210 9 0.9984107412981018 2210 10 1.1574143343698111 2210 11 0.49628546127347306 2210 12 0.42668425701094037 2210 13 0.6086711562789304 2210 14 0.776528700523329 2210 15 0.5197522721426893 2210 16 0.6981508460054329 2210 17 0.5864190530521824 2210 18 0.8113455408845951 2210 19 0.8119311825972986 2210 20 0.9376087129736348 2210 21 0.8081997223500323 2210 22 1.052922146769852 2210 23 1.1064712736830131 2210 24 0.7988376916469946 2210 25 0.7676876139346973 2211 1 -1.1101889234208997 2211 2 -0.13432496139958516 2211 3 0.5572973917805193 2211 4 0.7187544283319056 2211 5 0.941352082082517 2211 6 0.7254211513725082 2211 7 0.9434548288994051 2211 8 1.1523474050313212 2211 9 1.469890042959058 2211 10 1.3552454653508264 2211 11 1.0728919523178675 2211 12 1.02755152166106 2211 13 0.8992415985414581 2211 14 1.3343425710985812 2211 15 1.193655104546977 2211 16 1.052642795865367 2211 17 1.154744793693368 2211 18 1.0919035330183817 2211 19 1.1362711842950404 2211 20 1.1825494333833575 2211 21 1.1091914882876068 2211 22 1.1194737440647842 2211 23 1.3810305674211634 2211 24 1.3263793571346696 2211 25 1.2809979333936852 2212 1 1.2745111450427764 2212 2 0.2747558656653055 2212 3 0.6906721779239433 2212 4 0.5965726402617406 2212 5 0.7128120441391488 2212 6 0.6454206781894172 2212 7 0.6778571616920246 2212 8 0.8394306896908716 2212 9 1.0076892388435468 2212 10 0.6292140371216064 2212 11 0.5684484673835755 2212 12 0.6473422564404601 2212 13 0.5000562128011268 2212 14 0.6511319942736122 2212 15 0.6841005029153848 2212 16 0.05427971360987982 2212 17 0.6865781859281341 2212 18 0.9307957818357906 2212 19 0.6369225503894071 2212 20 0.6740328771507222 2212 21 0.9257080918385512 2212 22 0.8311344540464276 2212 23 1.0471747610950004 2212 24 1.0437574496449216 2212 25 0.6466250266743359 2213 1 0.18226234339634217 2213 2 0.008920828151627092 2213 3 -0.48902061371884287 2213 4 -1.3386405559556316 2213 5 -2.1192882007417957 2213 6 -1.1288288854535793 2213 7 -0.7115906513127115 2213 8 -0.4976835902549368 2213 9 -1.2314995278943215 2213 10 -1.0730879089003764 2213 11 -0.8524308896736912 2213 12 -1.1388379264387112 2213 13 -0.9124436243790939 2213 14 -7.915669704262314 2213 15 -3.671162483590116 2213 16 -2.5336038897871873 2213 17 -1.5766012610056421 2213 18 -6.267084793113408 2213 19 -3.730831155388742 2213 20 -5.9361855292995465 2213 21 -2.978468398288058 2213 22 -2.592454224117482 2213 23 -5.363954947652978 2213 24 -3.0804276373659647 2213 25 -2.699071483772183 2214 1 0.03504385132245247 2214 2 -0.13862314210908266 2214 3 -1.6635792734856452 2214 4 -2.059173521368969 2214 5 -0.7073924847716668 2214 6 -0.921139002707605 2214 7 -0.8400726538019043 2214 8 -0.8423895544891842 2214 9 -6.480844477455438 2214 10 -2.1518208775889676 2214 11 -1.5201974952023107 2214 12 -1.7928525668160233 2214 13 -1.8704896908012074 2214 14 -2.9503710012621838 2214 15 -2.541781833780078 2214 16 -1.8282128246751768 2214 17 -2.169018642769039 2214 18 -1.6434051291542815 2214 19 -4.969541770481104 2214 20 -2.667674213303215 2214 21 -4.457443326232507 2214 22 -6.125345944279935 2214 23 -6.254455078663064 2214 24 -6.209982284948829 2214 25 -3.608500525433392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19708.0 3 6.0 4 331.0 5 2350.0 6 2887.0 7 2480.0 8 1893.0 9 1543.0 10 1372.0 11 1242.0 12 1241.0 13 1132.0 14 1221.0 15 1241.0 16 1272.0 17 1407.0 18 1541.0 19 1898.0 20 2434.0 21 3223.0 22 4040.0 23 5014.0 24 6106.0 25 7224.0 26 8397.0 27 9389.0 28 10990.0 29 13357.0 30 16546.0 31 20966.0 32 27530.0 33 35454.0 34 46958.0 35 62755.0 36 85835.0 37 127783.0 38 201956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62976685611128 18.340859770450425 8.998213653524454 30.03115971991385 2 15.004300909740312 20.284629750412197 37.600889079740355 27.110180260107136 3 19.460160906564543 22.88867523502675 28.110576676380116 29.540587182028588 4 12.794067599760153 16.134756561995257 35.371031606696235 35.70014423154835 5 14.045458903212477 37.54525163351477 34.2450049353143 14.164284527958454 6 34.5429584746716 36.19781890723105 16.173072570732376 13.086150047364974 7 30.075282529233338 30.686792493654337 20.19335182377911 19.044573153333218 8 26.63897732467399 36.99489041025488 18.51313372843166 17.852998536639465 9 26.379763202527574 15.480695623006739 18.58335384640354 39.55618732806215 10 15.085237322416619 29.38709599021384 32.8185969393955 22.709069747974045 11 36.23665625763633 22.481615438306584 22.031232491295434 19.250495812761663 12 23.377075739165655 24.44660456325098 30.478466315647363 21.697853381936007 13 29.65417525063384 21.00167134228496 24.86762915101752 24.476524256063676 14 21.46277871216573 21.20042959330483 26.257337196971232 31.079454497558206 15 23.74633458798879 29.890321152194925 22.2430431425069 24.120301117309385 16 23.494143441140096 27.994162341975514 25.728897217257003 22.782796999627383 17 22.680063021551263 28.063221362523777 26.405597955444787 22.85111766048017 18 22.576555753865925 27.522539428812127 27.406028335281935 22.49487648204001 19 24.06603376210997 26.8278359200337 26.02752486796488 23.078605449891455 20 23.766495885732027 26.89698467021729 26.933975955664458 22.402543488386222 21 23.518976780731208 26.19407307204737 26.52159180042148 23.765358346799943 22 23.461163567438255 26.833143878540128 26.494420308833817 23.211272245187804 23 23.28822419207206 26.748496736967446 26.539511449639676 23.42376762132082 24 23.32379306620585 26.40430932066098 27.06771789610109 23.204179717032076 25 23.36345263601275 26.471235458424967 26.76068317220654 23.404628733355747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 120.0 2 137.5 3 155.0 4 155.0 5 155.0 6 267.5 7 380.0 8 380.0 9 380.0 10 650.5 11 921.0 12 921.0 13 921.0 14 1556.5 15 2192.0 16 2192.0 17 2192.0 18 3996.5 19 5801.0 20 5801.0 21 5801.0 22 9267.0 23 12733.0 24 12733.0 25 12733.0 26 18685.0 27 24637.0 28 24637.0 29 24637.0 30 32906.0 31 41175.0 32 41175.0 33 41175.0 34 49609.5 35 58044.0 36 58044.0 37 58044.0 38 65692.5 39 73341.0 40 73341.0 41 73341.0 42 80530.5 43 87720.0 44 87720.0 45 87720.0 46 93606.0 47 99492.0 48 99492.0 49 99492.0 50 99867.5 51 100243.0 52 100243.0 53 100243.0 54 92331.0 55 84419.0 56 84419.0 57 84419.0 58 73518.5 59 62618.0 60 62618.0 61 62618.0 62 52708.0 63 42798.0 64 42798.0 65 42798.0 66 34065.0 67 25332.0 68 25332.0 69 25332.0 70 18505.0 71 11678.0 72 11678.0 73 11678.0 74 8152.0 75 4626.0 76 4626.0 77 4626.0 78 3123.0 79 1620.0 80 1620.0 81 1620.0 82 1057.0 83 494.0 84 494.0 85 494.0 86 329.0 87 164.0 88 164.0 89 164.0 90 90.5 91 17.0 92 17.0 93 17.0 94 9.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08964226794937913 2 0.024435618221140997 3 0.022680573818517607 4 0.033210840234257924 5 0.019035481597684423 6 0.38273468318748466 7 0.16753923874274018 8 0.17928453589875826 9 0.18306463153517782 10 0.011340286909258803 11 0.0025650648961418727 12 0.0029700751429011154 13 0.0 14 0.06574666339058378 15 0.0018900478182098006 16 0.0018900478182098006 17 0.00310507855848753 18 0.0027000683117282868 19 0.0013500341558641434 20 9.450239091049003E-4 21 1.3500341558641434E-4 22 0.0 23 0.0 24 2.700068311728287E-4 25 1.3500341558641434E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.15876682024799 #Duplication Level Percentage of deduplicated Percentage of total 1 91.28384615381333 74.9976823060654 2 6.09153280028752 10.009456458334292 3 1.2193260240512989 3.005349674636722 4 0.4663519492490868 1.532596042180954 5 0.2559965779913539 1.0516181578986536 6 0.1510500982990675 0.7446053882597173 7 0.09984830713487718 0.5742389648303614 8 0.07486531065305375 0.4920673280695737 9 0.060004240187501987 0.44368869400120164 >10 0.27137947344792895 4.063621087709636 >50 0.015711275780077742 0.9210918740891784 >100 0.008599426784802326 1.3446945382054973 >500 0.0013229887288989707 0.6588283111526707 >1k 1.6537359111237134E-4 0.16046117456615883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1181 0.15943903380755534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.3500341558641434E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGTC 25 0.0060361004 18.99621 7 GCACCGT 60 1.4073667E-6 15.893629 6 TATGGAC 40 0.005134961 14.308155 3 CCGTCTC 40 0.005275509 14.2500515 9 GTCTTAT 75 9.2762093E-7 13.982594 1 TTAGAGC 55 0.0029736955 12.140252 3 GCTATAC 55 0.0029736955 12.140252 3 TAGGACG 315 0.0 12.109432 4 ACTGATC 55 0.0030657456 12.090952 8 ACCGATC 95 1.037266E-6 12.000042 8 GTAGGAC 415 0.0 11.952193 3 AGGACGT 330 0.0 11.847979 5 GTCCTAC 460 0.0 11.813357 1 TACCGAT 65 8.0281094E-4 11.689976 7 CGGTTTC 65 8.147386E-4 11.669445 13 TAGGACC 255 0.0 11.593 4 TGTAGGA 430 0.0 11.53604 2 GGACGTG 340 0.0 11.499509 6 GGCGAGG 165 7.2759576E-12 11.484878 19 GTCCTAT 75 2.0042014E-4 11.440304 1 >>END_MODULE