FastQCFastQC Report
Thu 26 May 2016
SRR1926587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611766
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAAC250.002672275821.8638883
CCTTATA450.001030025818.1968752
GTTATAG552.3507021E-416.973051
GTCCTAC3800.015.4416461
TAGGACC1250.015.4170664
CCTAGAC505.1725283E-415.3047223
TCCTACA4350.015.0594832
GCCTTAG550.00459129214.5483271
GTCTATG658.372139E-414.361811
TTAGACA450.00520989314.2750624
GCCATAT1653.6379788E-1113.74008751
GTCAGAG600.00701331213.6476562
TTATAGG700.00123092413.6476552
TGTAGGA3450.013.4498632
GGCGAGG1650.013.41474619
ATATACT808.357807E-613.3828714
GTTCTAG600.00817163213.3359671
CTGTAGG3500.013.3359671
GCCTACA600.00817163213.3359671
ATTGGAT500.00911424313.1183333
Produced by FastQC (version 0.11.5)