FastQCFastQC Report
Thu 26 May 2016
SRR1926587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611766
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA12270.20056688341620812No Hit
GTATCAACGCAGAGTACTTTTTTTT12260.20040342222352991No Hit
GTCCTACAGTGGACATTTCTAAATT11410.18650922084587898No Hit
CTTTAGGACGTGAAATATGGCGAGG9460.15463428827362097No Hit
GTCCTAAAGTGTGTATTTCTCATTT9170.1498939136859518No Hit
GTCCTACAGTGTGCATTTCTCATTT8840.1444996943275697No Hit
CTGTAGGACCTGGAATATGGCGAGA6660.10886515432371202No Hit
GGTATCAACGCAGAGTACTTTTTTT6600.10788438716764254No Hit
TATCAACGCAGAGTACTTTTTTTTT6320.10330747377265163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA350.002121004316.3394091
CCCTTAT400.005160284714.2969831
GTGTAGC954.658432E-914.0461591
TCTATAC706.9732796E-613.6217733
TAGGACC2550.013.0854344
GTCTTAG701.05584724E-412.2545581
AGGACGT6500.012.1737675
GTACAGT550.002986437912.1327686
GTCCTAA3300.012.1307741
TGGACAG1052.5876034E-711.80359655
GGACGTG6800.011.7759226
TATGGCG6450.011.75319216
GGCGAGA4200.011.73220519
TCTTAGA907.1195336E-611.6570612
TCAGGAC907.1391296E-611.6541843
TGGCGAG6200.011.61575618
ATGGCGA6450.011.60627717
AGGACCT6000.011.5993015
TGTAGGA7900.011.5899892
TTAGGAC3300.011.5578693
Produced by FastQC (version 0.11.5)