FastQCFastQC Report
Thu 26 May 2016
SRR1926586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835947
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTAG808.8717024E-716.6091351
GTCCTAC3750.015.5904421
GCCCTAT604.694757E-415.5018611
GTCTTAG953.139321E-715.3853041
TCCTACA4550.014.942712
GTGTTAG658.615355E-414.3094091
CTTAGAC550.001086610313.8776833
CTGCGGT400.006528095313.79913311
CCAACGT701.478289E-613.41589819
ATCCTAC600.00837747813.2873091
GTATTGG700.001507718713.2873091
CCTACAG5500.013.2829253
GTAGGAC2850.013.0081543
CTGTGCG500.001907263212.8801229
GTCCTAA1152.7072601E-612.7095991
TAGGACG2200.012.6334274
CCCTAGA854.5477596E-412.5057031
TGTAGGA3050.012.4832872
TATACTG1254.9203663E-912.4527115
CTGTAGG3000.012.4014871
Produced by FastQC (version 0.11.5)