##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926586_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835947 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.56487911314952 27.0 10.0 31.0 2.0 31.0 2 16.684897487520143 22.0 2.0 30.0 2.0 31.0 3 20.150596867983257 25.0 15.0 31.0 2.0 31.0 4 22.189561060689254 28.0 7.0 35.0 2.0 37.0 5 23.18999410249693 30.0 7.0 35.0 2.0 37.0 6 23.671206428158722 30.0 7.0 35.0 2.0 37.0 7 23.5969744493371 30.0 8.0 35.0 2.0 37.0 8 23.86097683226329 31.0 11.0 35.0 2.0 37.0 9 26.58027004104327 32.0 17.0 37.0 2.0 39.0 10 27.32444042505087 32.0 25.0 37.0 2.0 39.0 11 24.426967259886094 30.0 8.0 37.0 2.0 39.0 12 25.557025744455093 31.0 17.0 37.0 2.0 39.0 13 22.178130910213206 28.0 2.0 37.0 2.0 39.0 14 25.570173707184786 31.0 17.0 38.0 2.0 40.0 15 26.67884806094166 31.0 17.0 38.0 2.0 40.0 16 27.053472289511177 32.0 18.0 39.0 2.0 40.0 17 26.349997069192185 31.0 17.0 38.0 2.0 40.0 18 26.410875330613067 31.0 17.0 38.0 2.0 40.0 19 26.83926612572328 31.0 18.0 39.0 2.0 40.0 20 27.092746310471835 31.0 21.0 39.0 2.0 40.0 21 24.902816805371632 30.0 8.0 38.0 2.0 40.0 22 25.84998331233918 31.0 10.0 38.0 2.0 40.0 23 25.831053882602607 31.0 2.0 38.0 2.0 40.0 24 24.897187261871863 31.0 2.0 39.0 2.0 40.0 25 24.701183208983345 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.50682586524718 1101 2 10.25252060579744 1101 3 6.818613533780152 1101 4 8.239089585711213 1101 5 8.135069306025297 1101 6 7.43735972814337 1101 7 7.008156052090328 1101 8 5.433131568555538 1101 9 5.510586767333322 1101 10 5.092941606712106 1101 11 7.8979637702912875 1101 12 5.2492480148013065 1101 13 9.87773451496551 1101 14 7.332972236407997 1101 15 6.339386893783487 1101 16 5.9271260842426265 1101 17 1.803698229090351 1101 18 4.388574385369861 1101 19 5.044199721602563 1101 20 3.122951456061532 1101 21 -7.828599311565654E-5 1101 22 3.9626811025167434 1101 23 5.01172799348911 1101 24 6.366674603809095 1101 25 6.807681498602886 1102 1 5.7248939516563375 1102 2 10.792341274786533 1102 3 6.764655587969102 1102 4 8.013016545201701 1102 5 8.136347259814862 1102 6 6.825346962521486 1102 7 6.968235187813196 1102 8 7.060259442791899 1102 9 6.030949140291725 1102 10 5.417089134996402 1102 11 8.097198389618772 1102 12 5.331480514256263 1102 13 9.94075039313546 1102 14 7.512615893721559 1102 15 5.245802175420383 1102 16 5.866194360837287 1102 17 6.505785630924478 1102 18 6.41137252433516 1102 19 5.105787816917971 1102 20 5.49871254596361 1102 21 4.021449784922883 1102 22 5.520950228915883 1102 23 6.045242853470835 1102 24 7.424074443311088 1102 25 7.316717165342226 1103 1 4.7496988025865186 1103 2 9.170917097316462 1103 3 5.972887191611122 1103 4 7.433916695360459 1103 5 7.212909121019262 1103 6 6.569715053976957 1103 7 6.69581740259753 1103 8 6.271827589267371 1103 9 5.496092631692939 1103 10 4.6590844888628915 1103 11 5.58405376725085 1103 12 4.146603755240051 1103 13 8.444153800554755 1103 14 6.672906544524125 1103 15 5.758044125879458 1103 16 5.49278526037611 1103 17 3.279237906803939 1103 18 1.9696287723531007 1103 19 3.996598903448387 1103 20 4.638329622032586 1103 21 6.839932493740985 1103 22 2.251249056302086 1103 23 4.482713003209298 1103 24 5.867378050559974 1103 25 1.7000909995104116 1104 1 5.525548205708649 1104 2 10.351449696030876 1104 3 6.7406251775309265 1104 4 7.066102186636858 1104 5 7.244711998276685 1104 6 6.6624759967379 1104 7 6.874788936114516 1104 8 6.88332131439746 1104 9 5.666417088381035 1104 10 4.778720240316826 1104 11 7.83472716792425 1104 12 5.100169717896922 1104 13 9.162197877689742 1104 14 6.496245999681761 1104 15 5.931414190158609 1104 16 5.408472435154195 1104 17 5.95785743542347 1104 18 3.552334618695312 1104 19 4.2333757629525195 1104 20 3.3839066039732018 1104 21 2.656048196220322 1104 22 4.862360167413197 1104 23 5.164898494485698 1104 24 6.438559562650578 1104 25 5.501453105417973 1105 1 3.3312458067187514 1105 2 9.348269456131888 1105 3 6.2130354784568524 1105 4 5.526676681586352 1105 5 1.9789107566941944 1105 6 4.4930105085224135 1105 7 5.531792566165024 1105 8 2.567454311030456 1105 9 3.591268466748037 1105 10 3.36484055807777 1105 11 6.731307555129636 1105 12 3.8933646884356428 1105 13 4.529456400417015 1105 14 3.551011098587484 1105 15 3.8732031737444714 1105 16 4.205396098874733 1105 17 5.007931088622122 1105 18 2.2726536948737603 1105 19 2.3904898728010053 1105 20 1.5058776161785907 1105 21 2.311232855311232 1105 22 3.8081264237263284 1105 23 1.0207263038291323 1105 24 4.167018632515898 1105 25 4.8480926782294205 1106 1 6.476656154076874 1106 2 11.126586583496533 1106 3 7.787359974950654 1106 4 9.06437849176248 1106 5 8.808184953530464 1106 6 7.714247462207997 1106 7 7.625240615333318 1106 8 7.386151310378139 1106 9 6.311793224168131 1106 10 5.6657734205206935 1106 11 7.949395046975692 1106 12 5.425926964202116 1106 13 4.766692926020408 1106 14 5.462552245440062 1106 15 5.792870237916031 1106 16 5.831602628147397 1106 17 6.498075005778027 1106 18 6.214341510839187 1106 19 6.087304482634661 1106 20 5.550498814401028 1106 21 5.996972112486706 1106 22 6.184801804923328 1106 23 6.633945125176165 1106 24 7.471927817936642 1106 25 6.458592668036985 1107 1 3.5140747324743344 1107 2 -14.038449293868112 1107 3 -5.888551831278146 1107 4 1.3462466005526252 1107 5 4.032761569769097 1107 6 4.429538651316982 1107 7 4.416720737704662 1107 8 4.776800565958183 1107 9 3.011965432089692 1107 10 2.503828971076018 1107 11 5.321450732965818 1107 12 2.6156274047080927 1107 13 4.031114375121351 1107 14 3.3916804139072383 1107 15 3.176947048306449 1107 16 3.055077336555417 1107 17 3.6499522778468254 1107 18 -1.3463774250890062 1107 19 1.3933447033419597 1107 20 1.5774194813231368 1107 21 -1.5708661509305557 1107 22 1.2076461414770954 1107 23 -1.7145119038471712 1107 24 1.7633745101987692 1107 25 2.5166320521343692 1108 1 5.530848735761655 1108 2 11.073101861247398 1108 3 0.6137458229287986 1108 4 6.409896566074803 1108 5 7.683822576001123 1108 6 7.932676350106572 1108 7 8.013502807501656 1108 8 7.776323947994154 1108 9 5.956912804619325 1108 10 4.971646199579418 1108 11 7.230513413215444 1108 12 2.7731836859603973 1108 13 4.186740331935166 1108 14 5.4353332417393005 1108 15 5.269868035543151 1108 16 5.289107165293874 1108 17 0.2612211051237736 1108 18 3.76324904347112 1108 19 4.94264669004523 1108 20 5.203455225502022 1108 21 5.375700161416841 1108 22 1.9408680181982767 1108 23 4.970199024538704 1108 24 5.650834123439989 1108 25 6.248274454219519 1109 1 3.2936037612642046 1109 2 -14.038449293868112 1109 3 -5.834490563209815 1109 4 1.5193376664348364 1109 5 0.9877885976032843 1109 6 2.3369972509466557 1109 7 3.4263830017710823 1109 8 3.583079311030456 1109 9 3.1258920462934903 1109 10 2.2100701982292854 1109 11 0.5520935399781202 1109 12 1.279136468738674 1109 13 2.747141059507925 1109 14 3.375170662981425 1109 15 3.3565720184414403 1109 16 3.0770225193292795 1109 17 3.5668799522584855 1109 18 3.662509281994975 1109 19 1.8141996265888842 1109 20 0.9073335820876807 1109 21 -0.022929076506951418 1109 22 2.4643172381202696 1109 23 2.8732949591321635 1109 24 4.1129229885765035 1109 25 4.436575159290026 1110 1 3.168805817387316 1110 2 9.102375741693766 1110 3 6.069158974485774 1110 4 7.507376954227414 1110 5 8.340469478244692 1110 6 3.6716052216565984 1110 7 6.377041906788541 1110 8 7.261177639622204 1110 9 5.4929704359870115 1110 10 5.0272184993414015 1110 11 2.8177599614833575 1110 12 5.446550069540432 1110 13 9.966361850274769 1110 14 7.502181121406359 1110 15 6.7064059283082464 1110 16 6.296837355098059 1110 17 6.747329203842604 1110 18 6.964205341092349 1110 19 5.688541933908166 1110 20 5.932989658319418 1110 21 3.8567465021817853 1110 22 5.920652909916466 1110 23 6.295353830204192 1110 24 7.377391217271704 1110 25 7.701232310460334 1111 1 5.517174928702666 1111 2 9.938092261091672 1111 3 7.761144490409489 1111 4 8.705868482301277 1111 5 8.884898943691514 1111 6 8.174617970542073 1111 7 7.85580012034286 1111 8 8.284780048295872 1111 9 6.628765324992006 1111 10 5.973313175928531 1111 11 7.846910213753407 1111 12 7.265659287407942 1111 13 8.929831316636854 1111 14 6.879043591326539 1111 15 6.287274400844563 1111 16 6.289820319719237 1111 17 7.383978340632844 1111 18 7.069509495253566 1111 19 6.159600476576696 1111 20 5.763203143993604 1111 21 8.593289239359493 1111 22 7.842645595419807 1111 23 7.822745056621805 1111 24 7.611329261425638 1111 25 8.273348556804041 1112 1 -1.6471763034043683 1112 2 8.088089693819988 1112 3 6.422456256215629 1112 4 -0.7514882180944511 1112 5 4.088697222152472 1112 6 3.0337224328270196 1112 7 6.176100271078383 1112 8 6.31907879803456 1112 9 4.966500918013427 1112 10 3.5632960485588434 1112 11 -0.5923857303240787 1112 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1.0657059852801893 2204 6 3.618130891481851 2204 7 5.0152801844235775 2204 8 4.944536228093678 2204 9 3.44775825898121 2204 10 2.9978464674648073 2204 11 4.527892219756875 2204 12 4.536049476278382 2204 13 2.7362167887582807 2204 14 3.808096432402351 2204 15 4.033462545911931 2204 16 4.081523361366234 2204 17 -1.0350350058714213 2204 18 2.5270118235560624 2204 19 3.0873155688977647 2204 20 3.4622064847203973 2204 21 4.882610213400966 2204 22 4.393854581300541 2204 23 3.0384442855025995 2204 24 4.680881261396475 2204 25 4.854045881124275 2205 1 6.183511949102858 2205 2 10.741020904807385 2205 3 7.242930794406078 2205 4 7.992619010504676 2205 5 8.145844738482275 2205 6 7.689713047153759 2205 7 7.858868138736767 2205 8 7.558762258050322 2205 9 6.41349270101956 2205 10 5.774682066079976 2205 11 7.82171180457776 2205 12 7.068169807089067 2205 13 10.281945017966684 2205 14 7.624566734625013 2205 15 6.481611527773165 2205 16 6.217391649371418 2205 17 6.966022976039341 2205 18 6.719186359056987 2205 19 5.290630428816456 2205 20 5.669125236211702 2205 21 -1.1077085768079726 2205 22 3.8941147998300814 2205 23 4.936769522648117 2205 24 6.987122454259826 2205 25 7.5766376216620905 2206 1 4.35308891896365 2206 2 9.035283425025899 2206 3 6.2175276230969665 2206 4 1.8648480388389324 2206 5 5.826993891157656 2206 6 6.12273538234291 2206 7 6.4634031892449 2206 8 6.342100459812549 2206 9 4.908493769167393 2206 10 3.9809501319476404 2206 11 3.624346423902953 2206 12 4.789810918137203 2206 13 8.022680252457832 2206 14 0.39557415062281365 2206 15 2.3749462853036114 2206 16 3.5748427434599854 2206 17 4.704143646225809 2206 18 -0.9048038052633913 2206 19 1.0873706160257122 2206 20 2.667359522449164 2206 21 3.6257052672598817 2206 22 -2.221819349946042 2206 23 -2.7942625697444896 2206 24 0.9708892230908361 2206 25 2.776607103152614 2207 1 1.8576022627627076 2207 2 -14.038449293868112 2207 3 -6.021818859913113 2207 4 -4.351814618779947 2207 5 1.6692009352656214 2207 6 2.14742900669091 2207 7 3.3418815657071477 2207 8 3.747399365975511 2207 9 1.3767895862535298 2207 10 0.8825617691583538 2207 11 -0.8611260529289702 2207 12 -2.0074051146779084 2207 13 0.862260315252179 2207 14 -5.143950840515078 2207 15 -1.1707600245156016 2207 16 0.03551714969890796 2207 17 1.3326220226880565 2207 18 1.0358390771997676 2207 19 0.5107608778376331 2207 20 0.3566889142555141 2207 21 -3.279641114468987 2207 22 -3.900099595046566 2207 23 -0.8368386572514517 2207 24 0.7432692048602192 2207 25 1.4670759085407745 2208 1 5.008540288700733 2208 2 6.4892212338024144 2208 3 6.46423450114025 2208 4 8.462467383002053 2208 5 3.8600793136440004 2208 6 6.502703236965139 2208 7 6.962593623919204 2208 8 7.056273487349632 2208 9 5.320749342713288 2208 10 5.06240782400441 2208 11 6.83364426684135 2208 12 6.41749093521814 2208 13 9.26040777589964 2208 14 3.286947090382853 2208 15 4.9162548749992965 2208 16 5.172675599357358 2208 17 5.864852742418776 2208 18 0.9640021303628217 2208 19 3.79570172527848 2208 20 4.860172480239079 2208 21 7.19255223272436 2208 22 6.274239579292612 2208 23 2.0114684410556443 2208 24 3.8294673535583676 2208 25 5.0608475648124305 2209 1 3.2575422564745082 2209 2 5.059247845001394 2209 3 5.327309419344267 2209 4 0.4905236518119871 2209 5 5.09185944388889 2209 6 5.5835184754914735 2209 7 5.927548210646297 2209 8 4.66781195234239 2209 9 4.496860155949015 2209 10 3.600941522691631 2209 11 -0.30177063165798756 2209 12 3.4774658238741196 2209 13 2.4681421776326466 2209 14 4.0826313700168875 2209 15 4.418797443487435 2209 16 3.988923608906134 2209 17 1.6479018667100505 2209 18 3.6856717114183084 2209 19 4.300545717910332 2209 20 4.3759968481220675 2209 21 6.683073508660847 2209 22 5.4098165878633395 2209 23 0.9931940107086419 2209 24 -2.5064274087146217 2209 25 0.6237004685585639 2210 1 -1.7606270479723243 2210 2 -14.038449293868112 2210 3 -7.418574754666718 2210 4 -1.6081321475517107 2210 5 0.11224468594667414 2210 6 -0.597211650135101 2210 7 -0.5153550668441298 2210 8 -21.120045688969544 2210 9 -12.680787990631826 2210 10 -6.276077932101483 2210 11 -9.916587658447785 2210 12 -3.902708800313402 2210 13 -4.845624048667652 2210 14 -3.2648215792347877 2210 15 -4.987421766663616 2210 16 -4.446704814073399 2210 17 -4.1009195025296705 2210 18 -4.040422856899696 2210 19 -9.09748894823256 2210 20 -5.926370846350014 2210 21 -3.9428700480068457 2210 22 -8.980392300053229 2210 23 -6.932870962093826 2210 24 -9.39748663540935 2210 25 -6.963321259127561 2211 1 0.04661774208279468 2211 2 4.813705238078395 2211 3 0.7260359969684913 2211 4 1.3742265206155757 2211 5 0.9171711114490222 2211 6 -0.11083112099715109 2211 7 -20.886827225501644 2211 8 -13.606672732060183 2211 9 -9.215505655651665 2211 10 -7.629286523707769 2211 11 -9.842901521145746 2211 12 -5.148137524912549 2211 13 -7.235059990741975 2211 14 -5.208129603802313 2211 15 -5.639073750165881 2211 16 -11.921603460003425 2211 17 -8.185674966355588 2211 18 -6.382271972226288 2211 19 -9.091384469385723 2211 20 -7.127402124720369 2211 21 -4.734266541048353 2211 22 -6.269080539807597 2211 23 -8.117895267802862 2211 24 -22.30824178415077 2211 25 -22.117402113437247 2212 1 -0.9378930070370437 2212 2 -14.038449293868112 2212 3 -7.658848175265456 2212 4 -19.57228165174698 2212 5 -20.446870493305806 2212 6 -20.892406158144254 2212 7 -9.8845088329205 2212 8 -9.44771184043786 2212 9 -4.992812507942844 2212 10 -5.650904185289054 2212 11 -5.445558428899215 2212 12 -2.6576017109718784 2212 13 -5.617779640927653 2212 14 -3.64270247527908 2212 15 -3.5241163860829374 2212 16 -3.8716632857852353 2212 17 -3.878588424515435 2212 18 -2.939866631311194 2212 19 -3.609647983007857 2212 20 -3.657481517322182 2212 21 -1.458817618027254 2212 22 -2.8643895554725383 2212 23 -2.868094495694308 2212 24 -1.84920005308431 2212 25 -2.579535034611901 2213 1 -18.97344169328125 2213 2 -14.038449293868112 2213 3 -8.694455185851325 2213 4 -5.680772217784716 2213 5 -20.446870493305806 2213 6 -10.822437604685135 2213 7 -20.886827225501644 2213 8 -10.863756380793443 2213 9 -8.499398298189071 2213 10 -7.558078565349906 2213 11 -6.719262413423824 2213 12 -5.481021121312782 2213 13 -19.365074849582985 2213 14 -12.609722490248993 2213 15 -7.295220077041694 2213 16 -6.657995705370549 2213 17 -5.092403030874735 2213 18 -5.171172464717433 2213 19 -5.920769462890821 2213 20 -6.036906876745938 2213 21 -4.184074731166756 2213 22 -5.072364437122726 2213 23 -4.770412159221181 2213 24 -3.8247512181130325 2213 25 -4.7141002266447956 2214 1 -18.97344169328125 2214 2 -14.038449293868112 2214 3 -12.320516291807266 2214 4 -19.57228165174698 2214 5 -20.446870493305806 2214 6 -20.892406158144254 2214 7 -16.659140522405103 2214 8 -15.060847146164443 2214 9 -14.115097511166352 2214 10 -13.857207341512396 2214 11 -14.648559248149052 2214 12 -12.416267882147235 2214 13 -19.365074849582985 2214 14 -16.905895635246758 2214 15 -24.076666617922196 2214 16 -18.440967317564247 2214 17 -23.788943911377878 2214 18 -17.97535760407888 2214 19 -15.290154622872949 2214 20 -13.75639256255547 2214 21 -11.285498417457438 2214 22 -12.306873149361103 2214 23 -12.221158799768316 2214 24 -11.074179853367527 2214 25 -10.849642550595718 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 142532.0 3 3.0 4 595.0 5 2447.0 6 3461.0 7 3411.0 8 2890.0 9 2883.0 10 2652.0 11 2492.0 12 2382.0 13 2560.0 14 3950.0 15 8420.0 16 12564.0 17 13035.0 18 13549.0 19 15905.0 20 18571.0 21 21850.0 22 23032.0 23 24893.0 24 26340.0 25 27015.0 26 22476.0 27 17828.0 28 18925.0 29 22176.0 30 21639.0 31 25307.0 32 32410.0 33 40345.0 34 48914.0 35 55899.0 36 54824.0 37 56429.0 38 41343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.985398832511095 18.306962481813798 11.688215341117509 26.0194233445576 2 14.420064449715793 19.93092542034045 39.97504811350311 25.673962016440644 3 19.186510298091314 24.251732809290946 28.610409771094435 27.951347121523302 4 12.113688328107127 16.39747205581506 37.97767231613411 33.51116729994371 5 12.753402100463159 37.17018487820728 36.69201835013961 13.38439467118995 6 32.016731868457136 36.98589016500102 18.366101770640487 12.63127619590136 7 28.97461902190377 30.809372653873 21.789971418410758 18.426036905812467 8 25.09138249817106 37.953573949181916 19.970607930697682 16.98443562194935 9 26.08717000474245 15.6778585217937 19.719357326601294 38.51561414686256 10 13.804259286460868 29.996491666798182 34.81092026371983 21.388328783021123 11 33.84392452218367 22.54644530492972 24.087085500907563 19.522544671979045 12 22.885280073438604 24.92271956139706 31.86287064814134 20.329129717022997 13 28.17527927805133 21.208094094128207 25.263376098228633 25.353250529591833 14 20.31159782316696 21.877852778112057 27.93719907179489 29.87335032692609 15 22.661428377895735 30.788575932608346 23.55481113834255 22.995184551153365 16 22.126792692801132 28.480846517027576 27.191377081384587 22.200983708786705 17 21.4326430962544 28.211609230356316 28.563971175758677 21.791776497630604 18 21.31884682890094 27.95741502134715 29.78725730145331 20.936480848298604 19 22.57742209229342 27.283510117166585 27.221837103116552 22.91723068742344 20 22.739546583783874 27.894489981730437 28.083063528412588 21.282899906073098 21 22.093649926286975 26.415021201090877 26.948455902856367 24.54287296976578 22 22.14772525572789 27.868565468042224 28.119126525431763 21.86458275079812 23 22.174930479541462 27.52710758964606 27.956924860420163 22.341037070392307 24 22.097337478853515 27.49167593021562 28.241071885577345 22.16991470535352 25 22.40463729235781 27.4231409511719 28.102446794495613 22.06977496197468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 874.0 1 874.0 2 1792.5 3 2711.0 4 2711.0 5 2711.0 6 3889.0 7 5067.0 8 5067.0 9 5067.0 10 5976.5 11 6886.0 12 6886.0 13 6886.0 14 8397.5 15 9909.0 16 9909.0 17 9909.0 18 13617.5 19 17326.0 20 17326.0 21 17326.0 22 24167.0 23 31008.0 24 31008.0 25 31008.0 26 40601.5 27 50195.0 28 50195.0 29 50195.0 30 60759.5 31 71324.0 32 71324.0 33 71324.0 34 79115.5 35 86907.0 36 86907.0 37 86907.0 38 92798.5 39 98690.0 40 98690.0 41 98690.0 42 101460.0 43 104230.0 44 104230.0 45 104230.0 46 102563.5 47 100897.0 48 100897.0 49 100897.0 50 94539.5 51 88182.0 52 88182.0 53 88182.0 54 77086.0 55 65990.0 56 65990.0 57 65990.0 58 55563.5 59 45137.0 60 45137.0 61 45137.0 62 35938.0 63 26739.0 64 26739.0 65 26739.0 66 20411.5 67 14084.0 68 14084.0 69 14084.0 70 10171.5 71 6259.0 72 6259.0 73 6259.0 74 4311.0 75 2363.0 76 2363.0 77 2363.0 78 1595.5 79 828.0 80 828.0 81 828.0 82 536.0 83 244.0 84 244.0 85 244.0 86 159.5 87 75.0 88 75.0 89 75.0 90 45.5 91 16.0 92 16.0 93 16.0 94 11.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.926656833507389 2 35.853349554457395 3 5.525350291346222 4 13.516167891026583 5 13.114587408053383 6 14.320166230634237 7 9.177256452861245 8 7.286107851335073 9 0.11184919618109761 10 1.4587049178955125 11 5.558964862604926 12 5.653588086326047 13 15.746333200549795 14 1.589095959432835 15 2.868244039394842 16 2.934157309016002 17 2.836782714693635 18 1.5134930803029378 19 1.4651646575680037 20 0.2775295562996219 21 4.333289072154096 22 1.602135063586567 23 0.11256694503359664 24 8.85223584748794 25 0.11687343814859076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 835947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.1134804558444 #Duplication Level Percentage of deduplicated Percentage of total 1 96.91004164322734 91.20541310164944 2 2.386335364757536 4.491726534243973 3 0.34573144513595055 0.9761396881431936 4 0.13267870161554204 0.4994741756560451 5 0.0604776729313532 0.284588214471993 6 0.04401712892883852 0.24855631218999769 7 0.02563223149737579 0.16886369626475695 8 0.017388191045594754 0.13091705425056566 9 0.011681880679413047 0.09894802041085016 >10 0.05799072244474027 0.9488226851799049 >50 0.00478966244341234 0.31053069205124884 >100 0.003235355292901931 0.6360198254880229 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.1962480874983702E-4 0.0 8 0.0 0.0 0.0 1.1962480874983702E-4 0.0 9 0.0 0.0 0.0 1.1962480874983702E-4 0.0 10 0.0 0.0 0.0 1.1962480874983702E-4 1.1962480874983702E-4 11 0.0 0.0 0.0 1.1962480874983702E-4 1.1962480874983702E-4 12 0.0 0.0 0.0 1.1962480874983702E-4 1.1962480874983702E-4 13 0.0 0.0 0.0 1.1962480874983702E-4 2.3924961749967403E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCTAG 80 8.8717024E-7 16.609135 1 GTCCTAC 375 0.0 15.590442 1 GCCCTAT 60 4.694757E-4 15.501861 1 GTCTTAG 95 3.139321E-7 15.385304 1 TCCTACA 455 0.0 14.94271 2 GTGTTAG 65 8.615355E-4 14.309409 1 CTTAGAC 55 0.0010866103 13.877683 3 CTGCGGT 40 0.0065280953 13.799133 11 CCAACGT 70 1.478289E-6 13.415898 19 ATCCTAC 60 0.008377478 13.287309 1 GTATTGG 70 0.0015077187 13.287309 1 CCTACAG 550 0.0 13.282925 3 GTAGGAC 285 0.0 13.008154 3 CTGTGCG 50 0.0019072632 12.880122 9 GTCCTAA 115 2.7072601E-6 12.709599 1 TAGGACG 220 0.0 12.633427 4 CCCTAGA 85 4.5477596E-4 12.505703 1 TGTAGGA 305 0.0 12.483287 2 TATACTG 125 4.9203663E-9 12.452711 5 CTGTAGG 300 0.0 12.401487 1 >>END_MODULE