FastQCFastQC Report
Thu 26 May 2016
SRR1926586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12900.15431600328728973No Hit
GGTATCAACGCAGAGTACTTTTTTT8660.10359508437735884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3050.012.5082944
TGAGTTA2400.012.24636412
GTAGGAC6250.011.7531233
CTGTAGG6850.011.6921921
TGGCGAG5200.011.66061418
GTCCTAA2900.011.5074011
GTCCTAT752.002611E-411.4416451
TGTAGGA6950.011.2563552
CTTACAC1451.1314114E-911.1846783
TCTAACC600.00569150411.1298513
ACCGTGG600.005872613311.0831238
TTACACC951.2998333E-511.0435084
CTAGGAC1053.289946E-610.9027123
AGGACGT5100.010.8466535
GGCGAGG2200.010.76619219
GACGTGA2650.010.7532237
GCACCGT803.6358024E-410.7284796
TAGGACT1252.1156484E-710.6820834
AGGACCT5100.010.6596425
ATTTCTC6250.010.61115915
Produced by FastQC (version 0.11.5)