FastQCFastQC Report
Thu 26 May 2016
SRR1926585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757211
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8010.10578293236627571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG654.422461E-516.4500831
TAAGACT802.8998329E-816.0865924
GTACTAA500.00239940916.0388321
GTCTTAC500.00239940916.0388321
GTATTAG604.4880458E-415.5933081
GTATAAT604.4880458E-415.5933081
TAGGACC1950.014.8491624
TGTAGGA4400.013.9983492
ATTATAG550.001053525613.9341343
GTCCTAA3200.013.783371
GTCCTAC5650.013.7205331
TCCTAAA3500.013.6872752
TACTCTC450.007235613713.5856357
GTAGGAC5150.013.1804153
TCCTACA6150.013.1308832
TGGAGTG758.762391E-512.5011125
AATGTCC1102.6473572E-812.4075458
TAGAACA2000.012.3330554
CTTATAC902.2326209E-512.164723
CTGTAGG4850.012.1255771
Produced by FastQC (version 0.11.5)