##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 757211 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.584104034410487 27.0 10.0 31.0 2.0 31.0 2 16.619738751814225 20.0 2.0 31.0 2.0 31.0 3 20.157749953447585 25.0 15.0 31.0 2.0 31.0 4 22.24475476452402 28.0 7.0 35.0 2.0 37.0 5 23.156459692212607 30.0 7.0 35.0 2.0 37.0 6 23.68737907927909 31.0 2.0 35.0 2.0 37.0 7 23.590750794692628 31.0 7.0 35.0 2.0 37.0 8 23.884128730301065 31.0 11.0 35.0 2.0 37.0 9 26.6306894643633 32.0 17.0 37.0 2.0 39.0 10 27.373990869123666 32.0 25.0 38.0 2.0 39.0 11 24.441246891553345 30.0 8.0 37.0 2.0 39.0 12 25.59407483515163 31.0 17.0 37.0 2.0 39.0 13 22.1657199908612 28.0 2.0 37.0 2.0 39.0 14 25.605269865334762 31.0 17.0 38.0 2.0 40.0 15 26.73397639495464 31.0 17.0 38.0 2.0 40.0 16 27.100367004705426 32.0 18.0 39.0 2.0 40.0 17 26.41558429552661 31.0 17.0 38.0 2.0 40.0 18 26.50954489567637 31.0 17.0 38.0 2.0 40.0 19 26.922145874795795 31.0 18.0 39.0 2.0 40.0 20 27.185122772912703 31.0 22.0 39.0 2.0 40.0 21 24.988610836345483 30.0 8.0 38.0 2.0 40.0 22 25.955083853773914 31.0 10.0 38.0 2.0 40.0 23 25.956375435644755 31.0 2.0 38.0 2.0 40.0 24 24.978842092890886 31.0 2.0 39.0 2.0 40.0 25 24.776915549298675 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.3011649979780735 1101 2 10.097659062566127 1101 3 6.387931048711618 1101 4 7.931999136669951 1101 5 7.688364027212941 1101 6 7.180546105671752 1101 7 6.805040286214865 1101 8 5.35241751625523 1101 9 5.365902638570702 1101 10 4.86872465374816 1101 11 7.586147990916981 1101 12 5.346139371186087 1101 13 9.872039876328355 1101 14 7.047130033609328 1101 15 6.35911326097127 1101 16 5.7458231001711155 1101 17 1.9521034019582935 1101 18 4.333644930325278 1101 19 4.779039263634406 1101 20 3.05158712140409 1101 21 0.4807773273261766 1101 22 4.170595738373301 1101 23 4.985043431773256 1101 24 6.520077392789368 1101 25 6.945897914338495 1102 1 5.685174939983046 1102 2 10.786482804413893 1102 3 6.833607621977254 1102 4 8.180156254338254 1102 5 8.122661111882852 1102 6 6.731194389542903 1102 7 6.630446216011848 1102 8 6.931815472620876 1102 9 6.000215301880882 1102 10 5.296351247903338 1102 11 7.817182766690777 1102 12 5.2898544520179875 1102 13 9.654431055985484 1102 14 7.423046163875085 1102 15 5.178096542257784 1102 16 5.640175134953971 1102 17 6.399968066061568 1102 18 6.271057743048779 1102 19 5.014489167684143 1102 20 5.532510368275968 1102 21 4.517334022954952 1102 22 5.633334463332407 1102 23 6.171909221787345 1102 24 7.80863275715252 1102 25 7.173820502658764 1103 1 4.478631061326492 1103 2 8.905803858946218 1103 3 5.888715362437107 1103 4 7.500008186443704 1103 5 7.0484092760817205 1103 6 6.219279137345961 1103 7 6.3643766361394505 1103 8 6.055946928019935 1103 9 4.780789516398755 1103 10 4.240217866417844 1103 11 5.146962470554989 1103 12 3.8933791901906147 1103 13 8.061451641034239 1103 14 6.038683578104052 1103 15 4.99727314030762 1103 16 4.7633796612570904 1103 17 2.489116976618927 1103 18 1.8448364838697735 1103 19 3.547621465746019 1103 20 3.9420697760043915 1103 21 6.0200835113382425 1103 22 1.6326771863371015 1103 23 3.7885879264942197 1103 24 5.553124149953771 1103 25 1.3737410515933952 1104 1 5.89167646812 1104 2 10.646763354685682 1104 3 7.16025966032435 1104 4 7.880677032013917 1104 5 7.9646705110531215 1104 6 7.365640757516381 1104 7 7.241923035112254 1104 8 7.199467413604154 1104 9 5.884953167618551 1104 10 4.6576101857828505 1104 11 8.007779559384218 1104 12 4.489167680910818 1104 13 9.209027221750944 1104 14 6.9759895195071095 1104 15 6.220350063384544 1104 16 5.855330169019432 1104 17 6.365402724255205 1104 18 3.830738199756315 1104 19 4.67343797094037 1104 20 3.706571935453443 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6.006091645893296 2208 23 1.674648716098318 2208 24 2.962723290970235 2208 25 4.163757019877082 2209 1 3.5097127920957227 2209 2 4.992101935869293 2209 3 5.324917285514033 2209 4 0.742784628751398 2209 5 5.225464564543259 2209 6 5.915108464269039 2209 7 6.41923240537022 2209 8 4.698603178019937 2209 9 4.555452977937218 2209 10 3.5943645010332297 2209 11 -0.6432779140603948 2209 12 3.4541003440367675 2209 13 2.648687217957317 2209 14 4.097998481950206 2209 15 4.441443813384545 2209 16 4.061564757410935 2209 17 1.6547900535420048 2209 18 3.7234662915620795 2209 19 4.233102234976787 2209 20 4.158464006773624 2209 21 6.336621972876706 2209 22 5.250177186337101 2209 23 0.5527105226480664 2209 24 -3.048907100046229 2209 25 0.2816256669780124 2210 1 -1.9807839625106425 2210 2 -14.117273064130707 2210 3 -7.699732002593171 2210 4 -2.237684121248602 2210 5 -0.48178915195590477 2210 6 -0.9674828778582665 2210 7 -1.0154043199229115 2210 8 -21.324053071980064 2210 9 -13.123383914860101 2210 10 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-8.323793952630098 2213 10 -7.3536595516763406 2213 11 -6.49577315114761 2213 12 -5.141885578909889 2213 13 -19.521625282042685 2213 14 -12.634142579650133 2213 15 -7.059497523406311 2213 16 -6.284353029422256 2213 17 -4.9352131217331845 2213 18 -5.0570732406140415 2213 19 -5.910150305243363 2213 20 -5.939294778154402 2213 21 -3.9855414479531 2213 22 -4.91670469342581 2213 23 -4.732955094413747 2213 24 -3.602396595177474 2213 25 -4.512076703894131 2214 1 -19.110599707904278 2214 2 -14.117273064130707 2214 3 -12.080980181025165 2214 4 -19.737684121248602 2214 5 -20.58312918545674 2214 6 -21.063797785730962 2214 7 -16.443142116618308 2214 8 -14.856557852094786 2214 9 -13.477499937932762 2214 10 -13.505759483192392 2214 11 -14.399641441785061 2214 12 -11.979007925561703 2214 13 -19.521625282042685 2214 14 -16.64093107110907 2214 15 -24.279649936615456 2214 16 -18.212531502149293 2214 17 -24.013959946457994 2214 18 -17.81557828300771 2214 19 -14.734715642652274 2214 20 -13.273030974105918 2214 21 -10.791341698251403 2214 22 -11.775659333739378 2214 23 -11.761529737868186 2214 24 -10.528348125380838 2214 25 -10.328962287132887 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129106.0 3 4.0 4 510.0 5 2087.0 6 3095.0 7 3130.0 8 2738.0 9 2602.0 10 2394.0 11 2126.0 12 2070.0 13 2202.0 14 3586.0 15 7794.0 16 11753.0 17 12157.0 18 12623.0 19 14445.0 20 16965.0 21 19930.0 22 20602.0 23 22050.0 24 23606.0 25 24204.0 26 20244.0 27 15654.0 28 16675.0 29 19675.0 30 18876.0 31 21727.0 32 28514.0 33 35794.0 34 44094.0 35 50697.0 36 50558.0 37 52070.0 38 40854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49357671417524 18.632419330682307 11.699763730671304 26.174240224471145 2 13.94633267736233 20.46525299356259 40.81001300823855 24.77840132083653 3 18.795057221091064 25.265463736572354 28.98195604858527 26.957522993751315 4 12.012574173495336 17.069732299716208 38.26323451724265 32.6544590095458 5 12.2628108180922 37.64212981955559 37.029218890637345 13.065840471714866 6 30.9648441142644 37.708184389667714 18.833732243324732 12.493239252743154 7 27.901354811242072 31.19317287111505 22.796463376938465 18.109008940704406 8 24.299393807812507 38.69060202140241 20.56911219411862 16.440891976666457 9 26.369564182703748 15.46170402923429 20.078876531653737 38.08985525640822 10 14.067928295673667 29.942956978209324 35.06273927273166 20.926375453385344 11 33.28857231549775 22.54185459505091 25.064437605435373 19.10513548401596 12 23.35911375640941 24.87377798126226 32.18461348325021 19.582494779078115 13 28.297264641506093 21.863099229494303 25.327370032515596 24.512266096484016 14 20.02929359437778 22.656042139776133 28.02416049664736 29.29050376919873 15 22.137382742151253 32.043985145749836 23.579214947974705 22.239417164124205 16 21.359318662699568 28.32261752263553 28.528339260793228 21.789724553871675 17 20.887519730453743 28.828729447302155 29.103696046827398 21.180054775416707 18 20.81728936076517 27.68035036276832 31.159542874328032 20.34281740213848 19 22.605177342083017 27.2120455734933 27.45964008212891 22.723137002294777 20 22.859973326234456 28.1838662126564 28.3014744738421 20.65468598726705 21 21.76943172677697 26.541614346174985 27.058981519040966 24.629972408007077 22 22.224267818411978 28.68509674176842 28.008482206448736 21.08215323337087 23 21.46715849595431 28.208128404463483 28.535353535353536 21.789359564228675 24 22.37477445516656 27.40458781091019 28.826992391451324 21.393645342471928 25 22.03347476171147 27.696603735666 28.699471527677694 21.570449974944832 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 891.0 1 891.0 2 1872.0 3 2853.0 4 2853.0 5 2853.0 6 4083.5 7 5314.0 8 5314.0 9 5314.0 10 6186.0 11 7058.0 12 7058.0 13 7058.0 14 8436.5 15 9815.0 16 9815.0 17 9815.0 18 13954.0 19 18093.0 20 18093.0 21 18093.0 22 24744.0 23 31395.0 24 31395.0 25 31395.0 26 40542.5 27 49690.0 28 49690.0 29 49690.0 30 59738.5 31 69787.0 32 69787.0 33 69787.0 34 76886.0 35 83985.0 36 83985.0 37 83985.0 38 87123.5 39 90262.0 40 90262.0 41 90262.0 42 91223.5 43 92185.0 44 92185.0 45 92185.0 46 90620.0 47 89055.0 48 89055.0 49 89055.0 50 81822.5 51 74590.0 52 74590.0 53 74590.0 54 64502.0 55 54414.0 56 54414.0 57 54414.0 58 45492.0 59 36570.0 60 36570.0 61 36570.0 62 29245.0 63 21920.0 64 21920.0 65 21920.0 66 16701.0 67 11482.0 68 11482.0 69 11482.0 70 8256.0 71 5030.0 72 5030.0 73 5030.0 74 3441.5 75 1853.0 76 1853.0 77 1853.0 78 1265.0 79 677.0 80 677.0 81 677.0 82 453.0 83 229.0 84 229.0 85 229.0 86 136.5 87 44.0 88 44.0 89 44.0 90 28.5 91 13.0 92 13.0 93 13.0 94 9.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.585777280044798 2 36.64962606195631 3 5.950917247636392 4 14.003362338898933 5 13.726028808350646 6 15.027647511723947 7 9.423925431616814 8 7.541226949951863 9 0.11080134863333999 10 1.5826500143288993 11 5.98010330013695 12 5.965576305679659 13 16.494609824738415 14 1.7195999529853634 15 3.056611697400064 16 3.1935616360565287 17 3.029538662275112 18 1.6353433851330739 19 1.5901776387294957 20 0.2855214728787617 21 4.609019150540602 22 1.7254107507682799 23 0.11238611166504447 24 9.169438901442266 25 0.11621595565833039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 757211.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.31428756383883 #Duplication Level Percentage of deduplicated Percentage of total 1 96.64723722481564 89.21920849422179 2 2.4887295417781896 4.594905891766652 3 0.4046081156444714 1.120533298148001 4 0.1588981625066283 0.5867428266800991 5 0.0826384108545863 0.38143530117244695 6 0.05065843030070501 0.2805898141387181 7 0.029203443537032664 0.1887126559172375 8 0.02132856038275259 0.15751446851968984 9 0.013224185430402057 0.10987031309576971 >10 0.08949821203245727 1.503801178675323 >50 0.006767540182959976 0.4288903374661502 >100 0.006619750296753227 1.0695564735361431 >500 5.884222373784759E-4 0.3582389466619212 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 801 0.10578293236627571 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3206358597537543E-4 2 0.0 0.0 0.0 0.0 1.3206358597537543E-4 3 0.0 0.0 0.0 0.0 1.3206358597537543E-4 4 0.0 0.0 0.0 0.0 1.3206358597537543E-4 5 0.0 0.0 0.0 0.0 1.3206358597537543E-4 6 0.0 0.0 0.0 0.0 1.3206358597537543E-4 7 0.0 0.0 0.0 0.0 1.3206358597537543E-4 8 0.0 0.0 0.0 0.0 1.3206358597537543E-4 9 0.0 0.0 0.0 0.0 1.3206358597537543E-4 10 0.0 0.0 0.0 0.0 1.3206358597537543E-4 11 0.0 0.0 0.0 0.0 1.3206358597537543E-4 12 0.0 0.0 0.0 0.0 1.3206358597537543E-4 13 0.0 0.0 0.0 0.0 1.3206358597537543E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAGG 65 4.422461E-5 16.450083 1 TAAGACT 80 2.8998329E-8 16.086592 4 GTACTAA 50 0.002399409 16.038832 1 GTCTTAC 50 0.002399409 16.038832 1 GTATTAG 60 4.4880458E-4 15.593308 1 GTATAAT 60 4.4880458E-4 15.593308 1 TAGGACC 195 0.0 14.849162 4 TGTAGGA 440 0.0 13.998349 2 ATTATAG 55 0.0010535256 13.934134 3 GTCCTAA 320 0.0 13.78337 1 GTCCTAC 565 0.0 13.720533 1 TCCTAAA 350 0.0 13.687275 2 TACTCTC 45 0.0072356137 13.585635 7 GTAGGAC 515 0.0 13.180415 3 TCCTACA 615 0.0 13.130883 2 TGGAGTG 75 8.762391E-5 12.501112 5 AATGTCC 110 2.6473572E-8 12.407545 8 TAGAACA 200 0.0 12.333055 4 CTTATAC 90 2.2326209E-5 12.16472 3 CTGTAGG 485 0.0 12.125577 1 >>END_MODULE