FastQCFastQC Report
Thu 26 May 2016
SRR1926585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757211
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17750.23441286510629136No Hit
GTCCTACAGTGGACATTTCTAAATT16480.21764078968741868No Hit
GTATCAACGCAGAGTACTTTTTTTT15010.1982274425490385No Hit
GTCCTAAAGTGTGTATTTCTCATTT14340.18937918228868836No Hit
CTTTAGGACGTGAAATATGGCGAGG13530.17868203182468295No Hit
GTACATGGGAAGCAGTGGTATCAAC11570.15279756897350938No Hit
CCCATGTACTCTGCGTTGATACCAC11460.15134486952778023No Hit
GTCCTACAGTGTGCATTTCTCATTT10650.14064771906377482No Hit
GGTATCAACGCAGAGTACTTTTTTT8460.11172579373516761No Hit
CTGTAGGACCTGGAATATGGCGAGA8450.11159373014919223No Hit
TATCAACGCAGAGTACTTTTTTTTT8080.10670737746810335No Hit
CTGAAGGACCTGGAATATGGCGAGA7660.10116070685713757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC350.002197962716.25319710
TAGGACC4300.013.3129364
GTAAGGC655.229863E-513.210533
CTCTAGG1259.640644E-1112.97051
GTGTTAC603.9553267E-412.7161761
GTAGGAC10150.012.6899173
CGTTTCC604.16417E-412.63886914
CCAACGA751.52125285E-512.62968819
GGCGAGG3000.012.62968819
TGTAGGA10750.012.427062
TAGGACA2300.012.0298784
CTGTAGG10700.011.9436981
AGTACTG802.7385402E-511.9261725
GTGTTAG802.749751E-511.9214151
ATCCCGT802.9209377E-511.85128910
TCCAACG802.948717E-511.84033318
GACGTGG4900.011.6324917
ATGGCAA8500.011.58959717
TCCCGTT907.630626E-611.58562911
GTATAAA1406.348273E-1011.5808031