FastQCFastQC Report
Thu 26 May 2016
SRR1926584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403140
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG350.005884192419.0734211
GCTACTC409.629084E-519.0695193
GTTCTGA552.3256778E-416.9926851
GTATAGC500.002412021616.0216731
TGAACTG400.002958734815.54048255
TATACTG507.763608E-414.504455
GTCCTAC1151.6761078E-713.93189051
GACAGTC450.00737594813.5433547
GTATTAT600.00809834913.3513961
GTCCTGT600.00809834913.3513961
CTTCAAG600.00809834913.3513961
TCCTACA1653.6925485E-1013.2177332
GTAGCCC755.730988E-513.07624153
CACTGGG705.802318E-513.0596627
TACACTC653.852335E-412.7511645
TCTGGGG704.6999732E-412.4535633
AGACTGT600.003069572212.0849976
TATCTGT600.003069572212.0849976
CCCAGGA800.003383148511.926942
GACTGTC708.486929E-411.6085887
Produced by FastQC (version 0.11.5)