##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926584_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 403140 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.009547551719006 27.0 12.0 31.0 2.0 31.0 2 16.9762638289428 23.0 2.0 31.0 2.0 31.0 3 20.59846455325693 25.0 15.0 31.0 2.0 31.0 4 22.61124175224488 28.0 10.0 35.0 2.0 37.0 5 23.575653619090144 30.0 10.0 35.0 2.0 37.0 6 24.083422632336163 31.0 10.0 35.0 2.0 37.0 7 24.036081758198144 31.0 10.0 35.0 2.0 37.0 8 24.36161879247904 31.0 17.0 35.0 2.0 37.0 9 27.244912437366672 32.0 18.0 37.0 2.0 39.0 10 28.083060475269136 33.0 27.0 38.0 2.0 39.0 11 25.13593788758248 31.0 10.0 37.0 2.0 39.0 12 26.271935307833505 31.0 17.0 37.0 2.0 39.0 13 22.90029022175919 29.0 2.0 37.0 2.0 39.0 14 26.35704469911197 31.0 17.0 38.0 2.0 40.0 15 27.487857816143276 32.0 23.0 39.0 2.0 40.0 16 27.879939971225877 32.0 24.0 39.0 2.0 40.0 17 27.113221213474226 31.0 18.0 39.0 2.0 40.0 18 27.19775760281788 31.0 19.0 38.0 2.0 40.0 19 27.653237088852507 32.0 24.0 39.0 2.0 40.0 20 27.91567693605199 32.0 26.0 39.0 2.0 40.0 21 25.641400009922112 31.0 10.0 38.0 2.0 40.0 22 26.659465694299747 31.0 18.0 38.0 2.0 40.0 23 26.682631343949993 31.0 18.0 39.0 2.0 40.0 24 25.71226124919383 31.0 2.0 39.0 2.0 40.0 25 25.52707992260753 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.381916312228935 1101 2 10.480623707802977 1101 3 6.7905602541220595 1101 4 8.2274170603892 1101 5 7.892140191368668 1101 6 7.405626807087263 1101 7 7.330645838262527 1101 8 6.032911900738007 1101 9 5.484083867113277 1101 10 5.261439039403751 1101 11 8.245447169358364 1101 12 5.81634637842869 1101 13 10.799937584227258 1101 14 7.861407853424488 1101 15 7.091383527705062 1101 16 6.198755444118657 1101 17 1.9130050011070487 1101 18 4.615193315474539 1101 19 5.490801616377173 1101 20 3.6816036020022587 1101 21 0.6693274524411059 1101 22 4.627222512551654 1101 23 5.751568853234957 1101 24 7.516618868297897 1101 25 7.782322861613288 1102 1 5.590999771447162 1102 2 10.499572593598224 1102 3 6.612579744066078 1102 4 7.924659440650451 1102 5 7.853661868254225 1102 6 6.469833041104817 1102 7 6.746168680200462 1102 8 6.8933682458304695 1102 9 6.0130354187063375 1102 10 5.187406872110127 1102 11 8.315002770989437 1102 12 5.90675049363065 1102 13 10.269720272338787 1102 14 7.592417005996985 1102 15 5.683915344495645 1102 16 6.036163498777388 1102 17 6.823250550705701 1102 18 6.6945096864351115 1102 19 6.014449217652807 1102 20 6.114493623032441 1102 21 5.407802813557975 1102 22 6.278334742639625 1102 23 6.840924421296727 1102 24 8.241101752888596 1102 25 7.921817149894643 1103 1 5.134750779349115 1103 2 9.222180775074332 1103 3 6.213629037190842 1103 4 7.871861504833646 1103 5 7.164581612381518 1103 6 6.886473367933824 1103 7 6.757947425564112 1103 8 6.310311749566427 1103 9 5.612549475578881 1103 10 4.974273506523932 1103 11 5.6007910847022835 1103 12 4.6754544661082065 1103 13 9.140769027915848 1103 14 7.047621005351928 1103 15 5.7702648580149685 1103 16 5.590138664073304 1103 17 2.998658818189437 1103 18 1.8448531794201166 1103 19 4.217422931569917 1103 20 4.647196950452749 1103 21 7.199909463023118 1103 22 2.0978498746075864 1103 23 4.538749503272754 1103 24 5.889891732999331 1103 25 1.9039555146745144 1104 1 5.883890059474311 1104 2 10.909233461563637 1104 3 7.284322829574776 1104 4 7.699248046304696 1104 5 7.767315050044534 1104 6 7.189060744042326 1104 7 7.043456032762862 1104 8 7.029769235221096 1104 9 6.089784739011328 1104 10 4.827262551907342 1104 11 7.967311689814437 1104 12 4.933774758231316 1104 13 9.739527504702494 1104 14 7.289749330860531 1104 15 6.605142270638858 1104 16 6.108135534182853 1104 17 6.605502845315158 1104 18 3.968139564396644 1104 19 4.915138111538614 1104 20 3.4414483849858755 1104 21 2.3634253732995916 1104 22 4.936420557967004 1104 23 5.680293582563312 1104 24 6.694763929139132 1104 25 6.0663029325148905 1105 1 3.7384793401246554 1105 2 9.908912070127773 1105 3 6.543005124687735 1105 4 5.995202295288532 1105 5 2.086570178128472 1105 6 4.698116420382245 1105 7 5.805494032573808 1105 8 3.1796132658478093 1105 9 4.224192528027306 1105 10 3.8027895393352686 1105 11 7.11818606357377 1105 12 4.2148032108260765 1105 13 5.380013372932005 1105 14 3.96013405531464 1105 15 4.6273466377813115 1105 16 4.951958201731774 1105 17 5.724455487341814 1105 18 2.811385617898864 1105 19 3.0546787108391165 1105 20 2.0979849196299796 1105 21 2.7800337483823014 1105 22 4.646534935507411 1105 23 1.9360549966856304 1105 24 5.211930012889962 1105 25 5.962389519148786 1106 1 6.042262918737862 1106 2 11.036692819252185 1106 3 7.34256172309011 1106 4 8.903252535967606 1106 5 8.327816878495963 1106 6 7.277336930725408 1106 7 7.015576674350175 1106 8 7.114091621983832 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1.7225737051378687 1107 23 -1.1540893902113005 1107 24 2.5034033587690203 1107 25 2.999770237586013 1108 1 4.645861890460225 1108 2 10.41468897948127 1108 3 0.07681703531554973 1108 4 5.548936047730972 1108 5 7.144262812569046 1108 6 7.080913124145731 1108 7 7.099241377744143 1108 8 6.99634081660815 1108 9 5.148761383689525 1108 10 4.105975334934623 1108 11 6.327785927646492 1108 12 1.5785955819075497 1108 13 2.819381311085092 1108 14 4.577269387911702 1108 15 4.400382063828374 1108 16 4.392107721738569 1108 17 -0.9010223819980894 1108 18 2.7530388334285547 1108 19 4.194581862652896 1108 20 4.175429486786552 1108 21 3.984035107655089 1108 22 0.9356182759202909 1108 23 3.8375586922085247 1108 24 4.691982684710535 1108 25 5.323871126488861 1109 1 4.29109998569832 1109 2 -14.411893298999743 1109 3 -5.59723736492556 1109 4 2.446464679436822 1109 5 2.672412300212205 1109 6 3.5246744261348795 1109 7 4.328562504929192 1109 8 4.350258839513515 1109 9 4.036464819494228 1109 10 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3.4439830170102006 2207 8 3.595003439910876 2207 9 1.1377914874426978 2207 10 0.5858601672088461 2207 11 -1.457443300423087 2207 12 -2.276412340132758 2207 13 0.4081814201196252 2207 14 -6.181875031734958 2207 15 -1.7532544082230075 2207 16 -0.6070200019951848 2207 17 0.8182869523801557 2207 18 0.8089088097476171 2207 19 0.2532117755956378 2207 20 -0.06730182827213937 2207 21 -3.6706712657541125 2207 22 -4.464150025696487 2207 23 -1.1561650074694825 2207 24 0.8805562063555961 2207 25 1.454893154871911 2208 1 4.988255905422818 2208 2 7.3162862521224525 2208 3 6.715533535205065 2208 4 8.35360159904257 2208 5 4.069692908216005 2208 6 6.758197760655722 2208 7 6.810078209714849 2208 8 6.767400509148953 2208 9 5.19624394311375 2208 10 4.645168491284256 2208 11 6.577961122570574 2208 12 6.06422328480221 2208 13 8.76471102477555 2208 14 3.3274824628193684 2208 15 4.7862212632980565 2208 16 5.276949414973831 2208 17 5.957088669487121 2208 18 0.7414949084309228 2208 19 3.3931613632684474 2208 20 4.377323485965817 2208 21 6.917694629735962 2208 22 5.845798521508851 2208 23 2.011247886943206 2208 24 3.198845275070081 2208 25 4.423722763219818 2209 1 3.431134349959489 2209 2 5.42315824739201 2209 3 5.825611800513812 2209 4 1.5446482827750643 2209 5 5.87835240821417 2209 6 6.528356311260065 2209 7 6.930842205469201 2209 8 5.4991297280751255 2209 9 5.104211505797615 2209 10 4.112228014787721 2209 11 -0.563487538545413 2209 12 3.7860637499133674 2209 13 2.8699284671783793 2209 14 4.589116668909444 2209 15 5.058926833687252 2209 16 4.655070753747669 2209 17 2.2246818367897774 2209 18 4.2138467974859 2209 19 4.7819721051912545 2209 20 5.154441809450187 2209 21 7.38704959284366 2209 22 6.098840438072376 2209 23 1.3644168790432225 2209 24 -2.424232523805337 2209 25 1.0650355343112352 2210 1 -1.8901002546501857 2210 2 -14.411893298999743 2210 3 -7.696460926589973 2210 4 -1.9454536020713675 2210 5 -0.20989204108267145 2210 6 -0.9401746049602053 2210 7 -0.6565706256100903 2210 8 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-6.8462854483547275 2211 21 -4.26138167331446 2211 22 -5.8423611103712005 2211 23 -7.915668568049345 2211 24 -23.18270085959326 2211 25 -23.002711151992152 2212 1 -0.8318212441223984 2212 2 -14.411893298999743 2212 3 -7.883131641716345 2212 4 -20.1725829396108 2212 5 -20.92282579502589 2212 6 -21.403897002436548 2212 7 -9.9297906020717 2212 8 -9.22557064048973 2212 9 -5.0270542849107365 2212 10 -5.274047523194028 2212 11 -5.709270117897251 2212 12 -2.854837665887505 2212 13 -6.575046480949066 2212 14 -3.809043768450838 2212 15 -3.8345606830286307 2212 16 -3.910637410126956 2212 17 -4.107528322955112 2212 18 -3.073420528700268 2212 19 -3.709724522528134 2212 20 -3.680271028599556 2212 21 -1.6680620178274097 2212 22 -2.8438827268183715 2212 23 -3.089562693080328 2212 24 -2.0225645733922377 2212 25 -2.150922395603736 2213 1 -19.554138109539775 2213 2 -14.411893298999743 2213 3 -9.004397729071218 2213 4 -6.049893863980547 2213 5 -20.92282579502589 2213 6 -11.072804565461757 2213 7 -21.393163685546998 2213 8 -11.027752364968277 2213 9 -8.681182239329303 2213 10 -7.569293253513415 2213 11 -6.980883362854517 2213 12 -5.4524011205709115 2213 13 -20.084416157269338 2213 14 -13.089372458700362 2213 15 -7.651633679177689 2213 16 -6.844781970847325 2213 17 -5.260824530705676 2213 18 -5.375202842988848 2213 19 -6.249094342006181 2213 20 -6.168576470026554 2213 21 -4.316666945317998 2213 22 -5.254170044471156 2213 23 -5.157994972295253 2213 24 -4.191104220937799 2213 25 -5.0951481267820675 2214 1 -19.554138109539775 2214 2 -14.411893298999743 2214 3 -12.85796416478627 2214 4 -20.1725829396108 2214 5 -20.92282579502589 2214 6 -21.403897002436548 2214 7 -17.011079590483014 2214 8 -15.575679177375141 2214 9 -14.783853801555656 2214 10 -14.851093139829915 2214 11 -15.180843420003521 2214 12 -13.438473355680676 2214 13 -20.084416157269338 2214 14 -17.787146412888983 2214 15 -24.979364771614662 2214 16 -19.45528079560575 2214 17 -24.633933774403154 2214 18 -18.876341214242284 2214 19 -16.220464535421755 2214 20 -14.827055876414336 2214 21 -12.30681949628354 2214 22 -13.504568704849383 2214 23 -13.327979456033903 2214 24 -12.15527855611977 2214 25 -11.893021938098185 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 62615.0 3 4.0 4 231.0 5 1016.0 6 1483.0 7 1454.0 8 1253.0 9 1153.0 10 1079.0 11 986.0 12 1023.0 13 1143.0 14 1758.0 15 4026.0 16 6038.0 17 6479.0 18 6387.0 19 7316.0 20 8710.0 21 10200.0 22 10723.0 23 11893.0 24 12811.0 25 13126.0 26 11057.0 27 8453.0 28 9212.0 29 10810.0 30 10523.0 31 12158.0 32 15857.0 33 20392.0 34 25317.0 35 28959.0 36 28696.0 37 29424.0 38 19375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.658659445442076 17.492728096798068 11.293968479813655 25.554643977946196 2 14.905442696559144 19.280179604309254 40.68731388659359 25.127063812538005 3 19.620860345205784 22.94817684726851 29.028259726256202 28.402703081269507 4 11.927951659676731 16.36222892331866 37.26964980994304 34.44016960706157 5 13.05605662551064 36.72710952039267 36.67250798297347 13.54432587112322 6 33.27414927771196 35.92782649951537 17.335203937583138 13.462820285189531 7 28.800275177514067 31.610470941199914 21.120966178827167 18.468287702458852 8 26.003259591749494 37.028160735278405 19.335257026824838 17.63332264614727 9 26.093424058805997 15.856759709943379 19.415665044203834 38.63415118704679 10 14.367990070685819 29.52457353898315 34.18701217450971 21.920424215821313 11 34.254645990215074 22.758281637799104 23.095811406233064 19.891260965752757 12 22.849706706124824 24.815568355968924 31.02309358981134 21.311631348094913 13 27.98350747040655 20.909453648011585 25.162930231870778 25.944108649711083 14 20.7676374750562 21.345575791255147 27.373512844477 30.51327388921165 15 23.11257345504359 29.782483452611096 23.33482697256398 23.77011611978133 16 22.86094197615796 28.481327843435224 25.752636280559805 22.90509389984701 17 22.038515302177686 28.053907086057787 27.586674790961553 22.320902820802974 18 22.0584374936898 27.715404963350366 28.487268541889627 21.73888900107021 19 22.975986840445447 27.300020687950028 26.982132675355608 22.741859796248924 20 22.993095726814705 27.330223300366985 27.799465074329788 21.877215898488526 21 22.368112788100607 26.32980523435914 26.74161924644804 24.560462731092215 22 22.712219766340386 27.1057783391222 27.63751184085886 22.544490053678558 23 22.65281592553778 26.970949204567468 27.478307514267126 22.89792735562763 24 22.28551017848476 27.403927428194375 27.343941715700442 22.966620677620423 25 22.847663205576815 26.9460869651598 27.478785199681127 22.72746462958226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 521.0 1 521.0 2 1036.0 3 1551.0 4 1551.0 5 1551.0 6 2158.5 7 2766.0 8 2766.0 9 2766.0 10 3257.0 11 3748.0 12 3748.0 13 3748.0 14 4267.0 15 4786.0 16 4786.0 17 4786.0 18 6473.5 19 8161.0 20 8161.0 21 8161.0 22 10967.5 23 13774.0 24 13774.0 25 13774.0 26 17948.5 27 22123.0 28 22123.0 29 22123.0 30 26544.5 31 30966.0 32 30966.0 33 30966.0 34 34777.0 35 38588.0 36 38588.0 37 38588.0 38 41972.0 39 45356.0 40 45356.0 41 45356.0 42 47299.0 43 49242.0 44 49242.0 45 49242.0 46 49389.5 47 49537.0 48 49537.0 49 49537.0 50 46978.5 51 44420.0 52 44420.0 53 44420.0 54 39684.5 55 34949.0 56 34949.0 57 34949.0 58 29643.5 59 24338.0 60 24338.0 61 24338.0 62 19551.5 63 14765.0 64 14765.0 65 14765.0 66 11383.0 67 8001.0 68 8001.0 69 8001.0 70 5763.5 71 3526.0 72 3526.0 73 3526.0 74 2452.5 75 1379.0 76 1379.0 77 1379.0 78 915.0 79 451.0 80 451.0 81 451.0 82 302.0 83 153.0 84 153.0 85 153.0 86 90.0 87 27.0 88 27.0 89 27.0 90 18.5 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.528302822840702 2 36.35858510691075 3 6.1410428139108 4 14.121148980503051 5 13.229399216153197 6 14.778736915215557 7 9.136032147641018 8 7.158803393362108 9 0.11410428139108002 10 1.6713796695936893 11 6.152453242049908 12 6.121942749417076 13 16.07506077293248 14 1.7983826958376743 15 3.1242248350448976 16 3.3675646177506575 17 3.1110780374063602 18 1.7254551768616362 19 1.6803095698764696 20 0.3013841345438309 21 4.7095301880240115 22 1.802103487622166 23 0.11559259810487671 24 9.025648658034429 25 0.1173289676043062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 403140.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.455545525502 #Duplication Level Percentage of deduplicated Percentage of total 1 97.97395278351185 94.5013106302341 2 1.591144703089284 3.069494606929796 3 0.2302930263971883 0.666391184755789 4 0.07655863418410472 0.29538019299660667 5 0.04144483393703084 0.19987920333050746 6 0.023795789542135043 0.13771415168980025 7 0.011301537993116 0.07630672086822336 8 0.008314153804653448 0.06415569926486211 9 0.006700705658709805 0.05816881977450053 >10 0.03286335969135324 0.577767952040852 >50 0.002593194430963034 0.1730885642354481 >100 0.0010372777596257404 0.1803422738795238 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.4805278563278264E-4 13 0.0 0.0 0.0 0.0 2.4805278563278264E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTAG 35 0.0058841924 19.073421 1 GCTACTC 40 9.629084E-5 19.069519 3 GTTCTGA 55 2.3256778E-4 16.992685 1 GTATAGC 50 0.0024120216 16.021673 1 TGAACTG 40 0.0029587348 15.5404825 5 TATACTG 50 7.763608E-4 14.50445 5 GTCCTAC 115 1.6761078E-7 13.9318905 1 GACAGTC 45 0.007375948 13.543354 7 GTATTAT 60 0.008098349 13.351396 1 GTCCTGT 60 0.008098349 13.351396 1 CTTCAAG 60 0.008098349 13.351396 1 TCCTACA 165 3.6925485E-10 13.217733 2 GTAGCCC 75 5.730988E-5 13.0762415 3 CACTGGG 70 5.802318E-5 13.059662 7 TACACTC 65 3.852335E-4 12.751164 5 TCTGGGG 70 4.6999732E-4 12.453563 3 AGACTGT 60 0.0030695722 12.084997 6 TATCTGT 60 0.0030695722 12.084997 6 CCCAGGA 80 0.0033831485 11.92694 2 GACTGTC 70 8.486929E-4 11.608588 7 >>END_MODULE