FastQCFastQC Report
Thu 26 May 2016
SRR1926584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403140
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9530.23639430470804187No Hit
GGTATCAACGCAGAGTACTTTTTTT5810.14411866845264673No Hit
GTACATGGGAAGCAGTGGTATCAAC5360.1329562930991715No Hit
CCCATGTACTCTGCGTTGATACCAC4620.11460038696234559No Hit
TATCAACGCAGAGTACTTTTTTTTT4620.11460038696234559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG551.0969916E-515.5856245
CTGATCC400.005249552414.2583949
CTAGACT551.9121336E-413.85388854
TTAATCC707.0402803E-613.6064984
TGCGTTG1205.2750693E-1113.43618412
GTAGTAC500.001464597413.3376923
TGGACTG655.302881E-513.1878365
CCTTTAA957.1247996E-813.0287231
TCTGCGT1403.6379788E-1212.87323710
GTCCTAA751.4390973E-512.6946521
CTGCGTT1302.0008883E-1012.40263211
CTACAAC550.00299741712.1251743
TGAATAC550.00299741712.1251743
TAATCCC550.003003020812.1221525
TGTAGGA1501.2732926E-1112.0674352
GACAGTG951.0230051E-612.01005657
TGCTATA657.812961E-411.7254442
GGACGTG752.017568E-411.4294586
CTCTGCG1604.3655746E-1111.2878969
GTAGGAC1451.1477823E-911.1694963
Produced by FastQC (version 0.11.5)