FastQCFastQC Report
Thu 26 May 2016
SRR1926583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472302
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG350.00586808319.083871
TAGGACG608.40346E-616.0280484
ATAGAGC450.00458212414.5523253
CCCTTGA658.255959E-414.38631
GTCCTAC1757.2759576E-1213.7403851
CCTGAAC652.481516E-413.4329163
CCCTGGA853.6193256E-412.8447082
TGTAACT750.002067594612.737672
TTAGGAC600.002074879812.7332853
GTATATA1051.4027637E-512.7225791
GGTATCA2005.456968E-1212.6907731
TTAACCA600.002433041612.4662594
CTGGAGT705.548205E-412.2118464
GAACAGT707.234274E-411.8325536
TAGGACC1006.468248E-611.75390154
GTAGGAC1554.616595E-911.2663163
TAAACAG650.005592311711.1531425
GTCCTGG1205.5021577E-511.1322561
GTGTATA1205.5021577E-511.1322561
AGAACTG750.001286123911.0469225
Produced by FastQC (version 0.11.5)