FastQCFastQC Report
Thu 26 May 2016
SRR1926583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472302
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10090.21363449657210853No Hit
GGTATCAACGCAGAGTACTTTTTTT6360.1346596033893568No Hit
TATCAACGCAGAGTACTTTTTTTTT5070.10734657062642122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC508.417627E-515.2575313
AATACTG704.2420834E-714.9818845
AGGACGT1600.014.3008895
TAGGACG1650.013.8675294
GACGTGA1051.3515091E-913.576517
CTAGCAC500.001454813913.350343
TGTAGGA2550.013.0871752
TAGGACC1102.584784E-913.0008084
GGACGTG1850.012.8836846
TCTATAC751.4172472E-512.7146093
GATATAC905.197344E-712.7024311
GTGTAAA603.9866255E-412.7024311
AGAACAG1600.012.5132785
TTAGGAC1154.994945E-912.4382063
GTAGGAC2250.012.290793
ATACCAT550.002982262812.1340876
CTATACT1052.5818554E-711.8039094
CTAGGAC657.755373E-411.7365633
CTAAATT907.647397E-611.58102819
CCTAGAC751.996631E-411.4431493
Produced by FastQC (version 0.11.5)