FastQCFastQC Report
Thu 26 May 2016
SRR1926582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA1431.992475964887836No Hit
GTACATGGGAAGCAGTGGTATCAAC901.2540058520273094No Hit
CCCATGTACTCTGCGTTGATACCAC811.1286052668245785No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA570.7942037062839626No Hit
GAGTACATGGGAAGCAGTGGTATCA430.5991361293019367No Hit
CATGTACTCTGCGTTGATACCACTG420.5852027309460778No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA400.5573359342343598No Hit
GCGTTGATACCACTGCTTCCCATGT310.43193534903162883No Hit
GGTATCAACGCAGAGTACATGGGAA250.34833495889647487No Hit
ACTCTGCGTTGATACCACTGCTTCC230.3204681621847569No Hit
ACGCAGAGTACATGGGAAGCAGTGG220.3065347638288978No Hit
GTATCAACGCAGAGTACATGGGAAG200.2786679671171799No Hit
GAACAAAAAAAAAAAAAAAAAAAAA200.2786679671171799No Hit
AAACAAAAAAAAAAAAAAAAAAAAA170.2368677720496029No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA170.2368677720496029No Hit
GNACATGGGAAGCAGTGGTATCAAC170.2368677720496029No Hit
GGTATCAACGCAGAGTACTTTTTTT160.2229343736937439No Hit
GTGGTATCAACGCAGAGTACATGGG160.2229343736937439No Hit
TATCAACGCAGAGTACATGGGAAGC140.19506757698202593No Hit
CNCATGTACTCTGCGTTGATACCAC140.19506757698202593No Hit
GTATCAACGCAGAGTACTTTTTTTT130.18113417862616693No Hit
GAAAACAAAAAAAAAAAAAAAAAAA120.16720078027030794No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA120.16720078027030794No Hit
NNAANANAAAAANAAAAAAAAAAAA120.16720078027030794No Hit
NNANNNNAAAANNAAAAAAAAAANA120.16720078027030794No Hit
GNGTTGATACCACTGCTTCCCATGT110.1532673819144489No Hit
CTGCTTCCCATGTACTCTGCGTTGA110.1532673819144489No Hit
GNGTACATGGGAAGCAGTGGTATCA110.1532673819144489No Hit
AAANAAAAAAAAAAAAAAAAAAAAA110.1532673819144489No Hit
NNNNANNAAANNNAAAAAAANAAAA100.13933398355858995No Hit
ACACAAAAAAAAAAAAAAAAAAAAA100.13933398355858995No Hit
ANANNNAAAAAAAAAAAAAAAAAAA100.13933398355858995No Hit
ATACCACTGCTTCCCATGTACTCTG100.13933398355858995No Hit
ANAAAAANAAAAAAAAAAAAAAAAA100.13933398355858995No Hit
CATGGGAAGCAGTGGTATCAACGCA100.13933398355858995No Hit
ACANAAAAAAAAAAAAAAAAAAAAA90.12540058520273095No Hit
CCATGTACTCTGCGTTGATACCACT90.12540058520273095No Hit
GATACCACTGCTTCCCATGTACTCT90.12540058520273095No Hit
GCAGAGTACATGGGAAGCAGTGGTA90.12540058520273095No Hit
GTACTCTGCGTTGATACCACTGCTT80.11146718684687194No Hit
GCAGTGGTATCAACGCAGAGTACAT80.11146718684687194No Hit
AAAAACAAAAAAAAAAAAAAAAAAA80.11146718684687194No Hit
NNAAAANAAAAAAAAAAAAAAAAAA80.11146718684687194No Hit
GTTGATACCACTGCTTCCCATGTAC80.11146718684687194No Hit
NNANNNAAAAAANAAAAAAAAAANA80.11146718684687194No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA80.11146718684687194No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)