FastQCFastQC Report
Thu 26 May 2016
SRR1926582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT2483.4554827922530307No Hit
CCCATGTACTCTGCGTTGATACCAC2132.967813849797966No Hit
GGTATCAACGCAGAGTACTTTTTTT1832.549811899122196No Hit
GTACATGGGAAGCAGTGGTATCAAC1712.3826111188518877No Hit
TATCAACGCAGAGTACTTTTTTTTT1602.229343736937439No Hit
GAGTACATGGGAAGCAGTGGTATCA1111.5466072175003482No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1061.4769402257210533No Hit
ACGCAGAGTACATGGGAAGCAGTGG901.2540058520273094No Hit
CATGTACTCTGCGTTGATACCACTG881.2261390553155913No Hit
GCGTTGATACCACTGCTTCCCATGT841.1704054618921556No Hit
GAGTACTTTTTTTTTTTTTTTTTTT570.7942037062839626No Hit
GTATCAACGCAGAGTACATGGGAAG540.7524035112163856No Hit
ACTCTGCGTTGATACCACTGCTTCC490.6827365194370907No Hit
GGTATCAACGCAGAGTACATGGGAA470.6548697227253727No Hit
TATCAACGCAGAGTACATGGGAAGC430.5991361293019367No Hit
GTACTTTTTTTTTTTTTTTTTTTTT390.5434025358785008No Hit
ATCAACGCAGAGTACTTTTTTTTTT330.45980214574334677No Hit
GATACCACTGCTTCCCATGTACTCT320.4458687473874878No Hit
GTGGTATCAACGCAGAGTACATGGG320.4458687473874878No Hit
GCTTCCCATGTACTCTGCGTTGATA320.4458687473874878No Hit
GCAGAGTACTTTTTTTTTTTTTTTT300.41800195067576984No Hit
ACATGGGAAGCAGTGGTATCAACGC300.41800195067576984No Hit
CAGTGGTATCAACGCAGAGTACATG290.40406855231991085No Hit
GGGAAGCAGTGGTATCAACGCAGAG290.40406855231991085No Hit
GTGGTATCAACGCAGAGTACTTTTT280.39013515396405185No Hit
GTACTCTGCGTTGATACCACTGCTT270.3762017556081928No Hit
CTGCTTCCCATGTACTCTGCGTTGA250.34833495889647487No Hit
GCAGTGGTATCAACGCAGAGTACAT240.33440156054061587No Hit
CATGGGAAGCAGTGGTATCAACGCA230.3204681621847569No Hit
ATACCACTGCTTCCCATGTACTCTG220.3065347638288978No Hit
GCAGAGTACATGGGAAGCAGTGGTA210.2926013654730389No Hit
CCACTGCTTCCCATGTACTCTGCGT200.2786679671171799No Hit
AAGCAGTGGTATCAACGCAGAGTAC200.2786679671171799No Hit
ATGGGAAGCAGTGGTATCAACGCAG190.2647345687613209No Hit
CTTCCCATGTACTCTGCGTTGATAC190.2647345687613209No Hit
ATCAACGCAGAGTACATGGGAAGCA180.2508011704054619No Hit
TACCACTGCTTCCCATGTACTCTGC170.2368677720496029No Hit
AAAAAGTACTCTGCGTTGATACCAC170.2368677720496029No Hit
GTTGATACCACTGCTTCCCATGTAC170.2368677720496029No Hit
CCATGTACTCTGCGTTGATACCACT120.16720078027030794No Hit
CAACGCAGAGTACTTTTTTTTTTTT120.16720078027030794No Hit
TTCCCATGTACTCTGCGTTGATACC110.1532673819144489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA100.13933398355858995No Hit
AAAGTACTCTGCGTTGATACCACTG90.12540058520273095No Hit
CTGCGTTGATACCACTGCTTCCCAT90.12540058520273095No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA90.12540058520273095No Hit
GTACATGGTAAGCAGTGGTATCAAC80.11146718684687194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG908.199049E-49.54225353
TACATGG850.0058137998.9809452
GTACATG900.0093054548.4820031
Produced by FastQC (version 0.11.5)