FastQCFastQC Report
Thu 26 May 2016
SRR1926581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681449
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG501.1349107E-418.6684071
GCTCTAA500.002436488916.0014921
CCTCTAT604.566949E-415.5570071
GCCTTAT550.00459575114.5468111
GTCCTAG550.00459575114.5468111
TGTAGGA3750.014.5224422
GTCTTAG852.7912967E-514.1189631
TAGGACC1600.014.0005864
TACAAGG600.00712817113.6147892
CCTTATA700.001254007713.6147882
TCTATAC652.5503445E-413.3919623
GTCCTAC2100.013.3345781
GTCTAGG600.008179516513.3345781
GAATAGA600.008179516513.3345781
GCCTTAG802.6427952E-413.3345771
GTTATGG700.001467034713.3345771
TTTAGGA1851.2732926E-1113.2468212
CTGTAGG3650.013.1519121
ACAGCGT450.00994092512.947768
GGACCTG1900.012.5148886
Produced by FastQC (version 0.11.5)