FastQCFastQC Report
Thu 26 May 2016
SRR1926581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681449
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11690.17154621989319815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC402.6762072E-416.6909223
GTCCTAA1850.013.3983551
CTTAGAC655.2452036E-513.2060033
TTAGACT603.9550048E-412.7159544
TAGGACC2400.012.318584
TGGCGAG2950.011.56317718
CTACACT1501.6370905E-1011.4443584
GTAGGAC4050.011.3039043
GGCGAGA2100.011.28021619
AGAACAG1552.910383E-1011.0751855
GTCCTAC4650.011.0710971
TGTAGGA4400.011.0574752
AATCCCG600.005984377611.05461219
CTGTAGG4500.011.0164241
CTATAAG700.001446482510.8953661
GGACTGA1158.36606E-710.780126
CTATAGA803.6444535E-410.7251241
TATGGCG3200.010.65980416
ATGGCGA3200.010.36369917
ACTGTTC1659.240466E-1010.3626338
Produced by FastQC (version 0.11.5)