FastQCFastQC Report
Thu 26 May 2016
SRR1926580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482579
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC454.874941E-520.8341481
GTCATGA450.001167759817.8578411
TGTAGGA3200.016.6725732
GTGTAGG604.410606E-415.6256131
GTCTTAA550.004458796714.6109621
TAAGGGA754.3913988E-614.2402034
CTGTAGG3250.014.0115391
CACAAGG600.006898907513.680062
ACTGTGC1007.197741E-913.6132388
GCCATAT802.541762E-413.3933831
GATATAC802.541762E-413.3933831
ATGCTAG600.00793698313.3933821
CTCCTAC700.001417261413.3933821
TCCTACA3500.013.2892012
GTAGGAC3550.013.2134983
TAGAGTG550.001603390413.1776195
TCCAGGG853.5476254E-412.8753512
TGTATAA750.002031073212.7680562
GTCCTGA854.242397E-412.6055361
GTCCTAC3200.012.5562961
Produced by FastQC (version 0.11.5)