##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 482579 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.152598849100354 28.0 15.0 31.0 2.0 31.0 2 17.040977746648736 23.0 2.0 31.0 2.0 31.0 3 20.72058253674528 26.0 15.0 31.0 2.0 31.0 4 22.754769685377937 28.0 10.0 35.0 2.0 37.0 5 23.700538150230326 30.0 10.0 35.0 2.0 37.0 6 24.22334995928128 31.0 11.0 35.0 2.0 37.0 7 24.18572295934966 31.0 11.0 35.0 2.0 37.0 8 24.581685071252583 32.0 17.0 35.0 2.0 37.0 9 27.442630118591982 33.0 19.0 37.0 2.0 39.0 10 28.26987498419948 33.0 27.0 38.0 2.0 39.0 11 25.282279170871504 31.0 10.0 37.0 2.0 39.0 12 26.447982610101143 31.0 17.0 37.0 2.0 39.0 13 23.005406368698182 30.0 2.0 37.0 2.0 39.0 14 26.517144343205985 31.0 17.0 38.0 2.0 40.0 15 27.67946388052526 32.0 23.0 39.0 2.0 40.0 16 28.07436295404483 33.0 25.0 39.0 2.0 40.0 17 27.351312427602526 32.0 19.0 39.0 2.0 40.0 18 27.46669250008807 31.0 23.0 39.0 2.0 40.0 19 27.9139519125366 32.0 25.0 39.0 2.0 40.0 20 28.18985285310799 32.0 27.0 39.0 2.0 40.0 21 25.89825500073563 31.0 11.0 38.0 2.0 40.0 22 26.92028248224643 31.0 18.0 39.0 2.0 40.0 23 26.95256942386635 31.0 18.0 39.0 2.0 40.0 24 25.94384960804345 32.0 2.0 39.0 2.0 40.0 25 25.752231240895274 32.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.697122849190322 1101 2 10.598577315799478 1101 3 6.678039097378836 1101 4 7.993071435307346 1101 5 8.080412391918564 1101 6 7.477315308357454 1101 7 7.23293812837262 1101 8 5.55868834840696 1101 9 5.336521769912192 1101 10 5.066570669346131 1101 11 7.937679750884893 1101 12 5.525753255282304 1101 13 9.9186686838887 1101 14 7.167556584429413 1101 15 6.536342615178281 1101 16 6.1334942810027115 1101 17 1.76946687768109 1101 18 4.163611444023498 1101 19 4.749945671537191 1101 20 2.9896506376512377 1101 21 -0.07121298951177124 1101 22 3.930380467590439 1101 23 5.1214309077830436 1101 24 6.44282380230575 1101 25 7.253473953834085 1102 1 6.106977177753965 1102 2 11.482962083384717 1102 3 7.293750392650249 1102 4 8.524238814081755 1102 5 8.42065842753351 1102 6 7.127748308099374 1102 7 7.263613722671934 1102 8 7.228044297135202 1102 9 6.484897303992998 1102 10 5.745712125486985 1102 11 8.557786997178585 1102 12 5.8700554374863 1102 13 10.71168477083275 1102 14 8.088114035752788 1102 15 6.123213546844614 1102 16 6.36323505466715 1102 17 7.159433327369964 1102 18 7.163772026709243 1102 19 5.964937212270705 1102 20 6.138424760898449 1102 21 5.6419175125958745 1102 22 6.552943768711646 1102 23 6.607262353313974 1102 24 8.141202203934519 1102 25 8.098898781064317 1103 1 4.881235213915165 1103 2 9.057338540759183 1103 3 6.15126037299477 1103 4 7.485478757434908 1103 5 6.92770113242052 1103 6 6.463425140270878 1103 7 6.681504787364098 1103 8 6.351072644976153 1103 9 5.278537657547471 1103 10 4.443458765132451 1103 11 5.49320012850405 1103 12 4.111172320444634 1103 13 8.512578423699889 1103 14 6.425178044249812 1103 15 5.641730595836815 1103 16 5.212212908679433 1103 17 3.059744335659449 1103 18 1.512615588624005 1103 19 3.864100633545018 1103 20 4.286783955634196 1103 21 6.427578168673811 1103 22 1.8096171703304798 1103 23 4.115507582892416 1103 24 5.779584106243121 1103 25 1.6980399220588112 1104 1 5.622073815847649 1104 2 10.552107716471934 1104 3 6.981905726686765 1104 4 7.741124111126901 1104 5 7.82944573459158 1104 6 7.1950933974580416 1104 7 6.83973274871445 1104 8 7.174336989482892 1104 9 5.729488987620233 1104 10 4.7261111568776535 1104 11 7.919047080669149 1104 12 4.764181386411472 1104 13 9.442078602633444 1104 14 6.837267988183967 1104 15 6.380934937957772 1104 16 5.824878422218738 1104 17 5.940327062606844 1104 18 3.3836730859400035 1104 19 4.13644048801239 1104 20 3.3731193964071906 1104 21 2.86100892138764 1104 22 5.075252980899489 1104 23 5.514258030226305 1104 24 6.561106234359542 1104 25 5.535037416989027 1105 1 4.285860390718561 1105 2 10.398331468623397 1105 3 7.079062353192789 1105 4 5.911423594775783 1105 5 3.6262994351079314 1105 6 5.85516912895546 1105 7 6.827429822724778 1105 8 3.5506949929252336 1105 9 4.423285889513522 1105 10 3.9069028952597513 1105 11 7.291088388758649 1105 12 4.421813055946753 1105 13 5.893851408141192 1105 14 4.1424934615058255 1105 15 5.070050256374298 1105 16 5.374152088311682 1105 17 5.822716047116305 1105 18 3.181525065285957 1105 19 3.2283509871518064 1105 20 2.439776883498414 1105 21 3.447218904175937 1105 22 4.686240932706717 1105 23 2.020832901138526 1105 24 5.444438420245948 1105 25 5.931360997023454 1106 1 6.430468685649437 1106 2 11.357354510098052 1106 3 7.746331170397088 1106 4 9.287269984944754 1106 5 8.714145825276901 1106 6 7.5112612948540765 1106 7 7.3481794387002 1106 8 7.160151214123388 1106 9 6.025719069237024 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-1.7383205380149107 1107 24 1.8110839228914521 1107 25 2.595826605489062 1108 1 4.55638542124359 1108 2 10.166714863006792 1108 3 -0.540476108345672 1108 4 5.285195267547458 1108 5 6.660575381383879 1108 6 6.6781898859762165 1108 7 6.653927991222947 1108 8 6.3402725265027655 1108 9 4.7535313107589445 1108 10 3.8075317108885685 1108 11 6.17754260121286 1108 12 1.755096328230028 1108 13 2.5577464020361838 1108 14 4.0074641574765195 1108 15 3.953262710586749 1108 16 4.1346868868464846 1108 17 -0.43010080270054374 1108 18 3.0226752484361405 1108 19 4.113973697774515 1108 20 4.146160277881808 1108 21 3.9689380738951066 1108 22 0.9500187104844962 1108 23 3.6397523150579403 1108 24 4.517779079586607 1108 25 4.821030105692561 1109 1 3.820948049111202 1109 2 -14.549097102805174 1109 3 -5.9717079991765925 1109 4 2.355675634352245 1109 5 2.146342483189862 1109 6 3.124215475075925 1109 7 4.132843466189456 1109 8 4.2916802385423125 1109 9 3.810479779463183 1109 10 2.9302599513264056 1109 11 1.2136129504666826 1109 12 1.8648430854555222 1109 13 3.007567776827184 1109 14 3.885938002384144 1109 15 3.780750482029564 1109 16 3.478792254949422 1109 17 4.057604434549045 1109 18 4.311448342494774 1109 19 2.9684655505956528 1109 20 1.8376994663833308 1109 21 1.2151189215340317 1109 22 3.537672281430897 1109 23 4.203072095722799 1109 24 5.3219430201417985 1109 25 5.675540132590193 1110 1 3.3064037737159317 1110 2 10.031270995354337 1110 3 6.828117567916713 1110 4 7.838869694688142 1110 5 8.212984798823971 1110 6 3.8885223279037504 1110 7 6.659268560673947 1110 8 7.428605597657924 1110 9 5.696330587147177 1110 10 5.226236812875879 1110 11 2.982222481659612 1110 12 5.870345921853854 1110 13 10.220687516817787 1110 14 7.655643330042196 1110 15 6.954768924209528 1110 16 6.668798889363394 1110 17 7.3415170988253315 1110 18 7.427386590264046 1110 19 6.25201444026311 1110 20 6.449059968511559 1110 21 4.213592260880581 1110 22 6.287271607553347 1110 23 6.555872340227044 1110 24 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2204 8 5.664018911085652 2204 9 4.264682232080968 2204 10 3.824353556132465 2204 11 5.602106313799386 2204 12 5.675756202787241 2204 13 3.456799081507114 2204 14 4.924045516548375 2204 15 5.186652103196675 2204 16 5.259065360084271 2204 17 -0.27743676838251474 2204 18 3.7358223674767963 2204 19 4.578595419525513 2204 20 4.764632215745383 2204 21 6.498657615021493 2204 22 5.834154747662357 2204 23 4.579196109539758 2204 24 6.40139514666275 2204 25 6.474197861046626 2205 1 6.421425725857354 2205 2 11.148770195773025 2205 3 7.626038656510325 2205 4 8.009427657064204 2205 5 8.303839042420051 2205 6 7.739720246084776 2205 7 7.9079078186624905 2205 8 7.912314491909662 2205 9 6.621300784571073 2205 10 5.754512915571194 2205 11 8.269929282462098 2205 12 7.007440679508029 2205 13 10.40085208518926 2205 14 7.722207747220111 2205 15 6.6237117323390855 2205 16 6.594615189191849 2205 17 7.189096548131882 2205 18 7.0837701566874465 2205 19 5.6642683872872155 2205 20 6.110443098799703 2205 21 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1.0302124111155386 2207 10 0.6349571368239282 2207 11 -1.1273903245809933 2207 12 -1.291508924501052 2207 13 1.2416344418844893 2207 14 -5.227571502228013 2207 15 -1.3287248934207234 2207 16 -0.43387314032281665 2207 17 0.8290900616227717 2207 18 0.6555925518832453 2207 19 -0.029832551164183485 2207 20 0.04789798094401476 2207 21 -4.186747667883353 2207 22 -4.458553769280037 2207 23 -1.4147773164584443 2207 24 0.541424859854903 2207 25 1.0458914290638184 2208 1 4.784922224407261 2208 2 6.785657694636189 2208 3 6.30112631907765 2208 4 8.316847901812032 2208 5 3.1887130811633675 2208 6 6.1008066556079115 2208 7 6.597903170272751 2208 8 6.607334651773847 2208 9 4.963149428959152 2208 10 4.4617856244708385 2208 11 6.449629966583807 2208 12 5.7565337382495265 2208 13 8.586772516883194 2208 14 2.689691755748896 2208 15 4.149103561278345 2208 16 4.681034817522772 2208 17 5.449300268864707 2208 18 -0.4030122481468794 2208 19 2.523020496746689 2208 20 3.6600924485303956 2208 21 6.453803125924338 2208 22 5.362539440169403 2208 23 1.2372934555095298 2208 24 2.8440460961521303 2208 25 4.2622010823113 2209 1 3.0816694465276164 2209 2 4.85507980137173 2209 3 5.255828937959372 2209 4 1.0184871258393144 2209 5 5.219564440372938 2209 6 5.6409830987694285 2209 7 6.178041265336223 2209 8 4.757028443258683 2209 9 4.011603193830826 2209 10 3.4142879426447976 2209 11 -0.42232824865799046 2209 12 2.7437379418716397 2209 13 1.915466009755793 2209 14 3.5152052902176543 2209 15 3.7362826186066584 2209 16 3.755158757318352 2209 17 1.0937683461686056 2209 18 3.3518978276587177 2209 19 3.8377577965586163 2209 20 4.121380253101783 2209 21 6.129861234818264 2209 22 4.5684325788983635 2209 23 0.347235147540772 2209 24 -3.5526707408632134 2209 25 -0.08427817461571863 2210 1 -1.583227068966508 2210 2 -14.549097102805174 2210 3 -7.541421046541608 2210 4 -1.5038494084494118 2210 5 0.09354436016959156 2210 6 -0.3303486096140702 2210 7 -0.5473438445110403 2210 8 -21.90759072136048 2210 9 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-4.024019191062159 2211 22 -5.595092400626616 2211 23 -7.4161194798138546 2211 24 -23.462990151182623 2211 25 -23.269781860119405 2212 1 -0.00685988301967555 2212 2 -14.549097102805174 2212 3 -7.6388178863281695 2212 4 -20.369719262367074 2212 5 -21.142843422034925 2212 6 -21.614545156758826 2212 7 -9.730994471543827 2212 8 -9.169566769264673 2212 9 -4.227487421008291 2212 10 -4.610855872918179 2212 11 -5.128153698495417 2212 12 -2.1246505881935356 2212 13 -5.942778189805772 2212 14 -2.967912013258932 2212 15 -2.8860010695452942 2212 16 -3.0233432068123527 2212 17 -3.5269263823105526 2212 18 -2.4615869963051473 2212 19 -3.182299141162993 2212 20 -3.110070849606803 2212 21 -0.8943225842757343 2212 22 -2.4430285283711264 2212 23 -2.6628540107519783 2212 24 -1.3237685942963964 2212 25 -1.548224973891859 2213 1 -19.759853894995725 2213 2 -14.549097102805174 2213 3 -8.580657041580253 2213 4 -5.325702756177254 2213 5 -21.142843422034925 2213 6 -10.701202653320037 2213 7 -21.62171303441808 2213 8 -10.852570088623205 2213 9 -8.107510730647611 2213 10 -7.220147605821012 2213 11 -6.540168046690361 2213 12 -4.886439252954798 2213 13 -20.329005734715953 2213 14 -12.831093490744125 2213 15 -6.832866623169821 2213 16 -6.075176266964402 2213 17 -4.59760071452645 2213 18 -4.765751020389029 2213 19 -5.580216511374427 2213 20 -5.59819364116262 2213 21 -3.7503979164175014 2213 22 -4.576383551474851 2213 23 -4.390619250561219 2213 24 -3.3364426958868876 2213 25 -4.20100606919781 2214 1 -19.759853894995725 2214 2 -14.549097102805174 2214 3 -12.638922743677554 2214 4 -20.369719262367074 2214 5 -21.142843422034925 2214 6 -21.614545156758826 2214 7 -17.107555061243712 2214 8 -15.435161511226353 2214 9 -14.209788839036616 2214 10 -14.261689823496901 2214 11 -15.37667230955943 2214 12 -12.862114557423482 2214 13 -20.329005734715953 2214 14 -17.62391743906901 2214 15 -25.197378315054276 2214 16 -19.172097858462852 2214 17 -24.990998238597978 2214 18 -18.70002145376876 2214 19 -15.54009695834532 2214 20 -14.323377470344465 2214 21 -11.817131958076565 2214 22 -12.89072479009507 2214 23 -12.844451322834221 2214 24 -11.565821745817496 2214 25 -11.292136554605246 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 73317.0 3 5.0 4 271.0 5 1118.0 6 1718.0 7 1673.0 8 1424.0 9 1350.0 10 1225.0 11 1160.0 12 1087.0 13 1238.0 14 2072.0 15 4832.0 16 7662.0 17 7993.0 18 8013.0 19 8627.0 20 10195.0 21 12135.0 22 12431.0 23 14081.0 24 15206.0 25 15781.0 26 12978.0 27 9997.0 28 10755.0 29 12580.0 30 12286.0 31 14213.0 32 18833.0 33 24061.0 34 30078.0 35 34749.0 36 34700.0 37 36806.0 38 25929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73430569853472 18.697264741819723 11.886539220119493 25.68189033952607 2 13.670469122679238 20.49586126120806 42.45200998677834 23.381659629334354 3 18.649487112017212 24.629236936779563 29.961326101233187 26.759949849970038 4 11.14120494303302 17.575820709670698 39.380982259443826 31.901992087852456 5 12.016622555418497 37.419911138788756 38.09654974191926 12.466916563873486 6 30.693431904410794 37.97297759200754 18.638088215905817 12.695502287675847 7 26.85678148743425 32.22781816189363 22.8895748100526 18.02582554061952 8 23.898348974471162 38.979248332455285 20.810824084078185 16.311578608995365 9 25.85873923658865 15.849674219464688 20.723417407737003 37.56816913620966 10 13.244180015770143 30.491615259089972 35.96773445439097 20.296470270748912 11 32.93310804534658 23.060540248848863 25.1485087203259 18.857842985478655 12 22.558115868928475 25.397099294733955 32.7855796543577 19.259205181979876 13 27.69413009305132 22.126447485726917 25.79436328011503 24.385059141106733 14 19.40090560780216 22.888656681760132 28.91675374433995 28.79368396609776 15 21.80060192736629 32.31956092695102 23.97884263837694 21.900994507305754 16 20.8750437221439 29.163045507509477 28.77249678655654 21.189413983790082 17 20.384011231001672 29.172569648046775 29.635463257418497 20.807955863533056 18 20.250766770871568 28.50805584032603 31.38149540359359 19.85968198520881 19 21.787854380764024 28.063498623170375 28.062655235958655 22.08599176010694 20 22.103436451113442 28.62489998233454 29.14197832344414 20.129685243107872 21 21.239626047739236 27.21889883123586 27.469986041031962 24.071489079992944 22 21.318480858718512 29.306471636446553 28.806509640314502 20.568537864520433 23 20.922402902501105 28.767370510738132 29.12375250743154 21.18647407932922 24 21.282094563769412 28.199875910144346 29.470200186134782 21.04782933995146 25 21.381911267754646 28.368636903934597 29.255254046720612 20.99419778159014 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 622.0 1 622.0 2 1298.0 3 1974.0 4 1974.0 5 1974.0 6 2779.0 7 3584.0 8 3584.0 9 3584.0 10 4243.5 11 4903.0 12 4903.0 13 4903.0 14 5953.5 15 7004.0 16 7004.0 17 7004.0 18 9751.5 19 12499.0 20 12499.0 21 12499.0 22 17166.5 23 21834.0 24 21834.0 25 21834.0 26 27908.0 27 33982.0 28 33982.0 29 33982.0 30 40516.5 31 47051.0 32 47051.0 33 47051.0 34 51750.0 35 56449.0 36 56449.0 37 56449.0 38 58300.5 39 60152.0 40 60152.0 41 60152.0 42 59996.5 43 59841.0 44 59841.0 45 59841.0 46 57812.0 47 55783.0 48 55783.0 49 55783.0 50 50823.0 51 45863.0 52 45863.0 53 45863.0 54 38651.5 55 31440.0 56 31440.0 57 31440.0 58 25660.5 59 19881.0 60 19881.0 61 19881.0 62 15438.5 63 10996.0 64 10996.0 65 10996.0 66 8153.5 67 5311.0 68 5311.0 69 5311.0 70 3772.5 71 2234.0 72 2234.0 73 2234.0 74 1526.5 75 819.0 76 819.0 77 819.0 78 534.5 79 250.0 80 250.0 81 250.0 82 169.0 83 88.0 84 88.0 85 88.0 86 52.0 87 16.0 88 16.0 89 16.0 90 9.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.847059237969328 2 36.83873521226577 3 6.286224638867418 4 14.463124172415293 5 13.484631531832095 6 15.125606377401422 7 9.234342978040901 8 7.172918838159141 9 0.10485329863089775 10 1.7126729509572527 11 6.303009455446674 12 6.330776929787661 13 16.556460185793416 14 1.8367977056606275 15 3.1938812090870092 16 3.4332202603097106 17 3.1706725738169297 18 1.7640635004838585 19 1.7201328694369213 20 0.29300902028476167 21 4.843973732798154 22 1.8531680823243448 23 0.10609661837750917 24 9.156428273919918 25 0.1081688179551949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 482579.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.95048551669147 #Duplication Level Percentage of deduplicated Percentage of total 1 97.70907730111308 92.77524329128627 2 1.7298022119166045 3.284911197386569 3 0.26296045808992125 0.7490466950198886 4 0.09687698935027955 0.3679406869681757 5 0.0548033382918341 0.26018017893725687 6 0.03176718549972992 0.18097858120188945 7 0.01409960777776835 0.0937135222865817 8 0.018372073954958178 0.1395549873577473 9 0.012491187392920655 0.10674398768740127 >10 0.06064365272875073 1.0271310305814332 >50 0.0062187286406685995 0.4135407454691155 >100 0.0028872652435331467 0.6010150958176605 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0721995776857262E-4 0.0 4 0.0 0.0 0.0 2.0721995776857262E-4 0.0 5 0.0 0.0 0.0 2.0721995776857262E-4 0.0 6 0.0 0.0 0.0 2.0721995776857262E-4 0.0 7 0.0 0.0 0.0 2.0721995776857262E-4 0.0 8 0.0 0.0 0.0 2.0721995776857262E-4 0.0 9 0.0 0.0 0.0 2.0721995776857262E-4 0.0 10 0.0 0.0 0.0 2.0721995776857262E-4 0.0 11 0.0 0.0 0.0 2.0721995776857262E-4 0.0 12 0.0 0.0 0.0 2.0721995776857262E-4 0.0 13 0.0 0.0 0.0 2.0721995776857262E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAC 45 4.874941E-5 20.834148 1 GTCATGA 45 0.0011677598 17.857841 1 TGTAGGA 320 0.0 16.672573 2 GTGTAGG 60 4.410606E-4 15.625613 1 GTCTTAA 55 0.0044587967 14.610962 1 TAAGGGA 75 4.3913988E-6 14.240203 4 CTGTAGG 325 0.0 14.011539 1 CACAAGG 60 0.0068989075 13.68006 2 ACTGTGC 100 7.197741E-9 13.613238 8 GCCATAT 80 2.541762E-4 13.393383 1 GATATAC 80 2.541762E-4 13.393383 1 ATGCTAG 60 0.007936983 13.393382 1 CTCCTAC 70 0.0014172614 13.393382 1 TCCTACA 350 0.0 13.289201 2 GTAGGAC 355 0.0 13.213498 3 TAGAGTG 55 0.0016033904 13.177619 5 TCCAGGG 85 3.5476254E-4 12.875351 2 TGTATAA 75 0.0020310732 12.768056 2 GTCCTGA 85 4.242397E-4 12.605536 1 GTCCTAC 320 0.0 12.556296 1 >>END_MODULE