FastQCFastQC Report
Thu 26 May 2016
SRR1926580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482579
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8990.18629074203394677No Hit
GTCCTACAGTGGACATTTCTAAATT8280.17157812503237813No Hit
GTCCTACAGTGTGCATTTCTCATTT8200.16992036537022956No Hit
CTGTAGGACGTGGAATATGGCAAGA8090.16764094583477523No Hit
GTCCTAAAGTGTGTATTTCTCATTT6120.12681861415436643No Hit
CTGTAGGACCTGGAATATGGCGAGA6050.1253680744499864No Hit
CTTTAGGACGTGAAATATGGCGAGG5880.1218453351679207No Hit
GGTATCAACGCAGAGTACTTTTTTT4990.10340275892651773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG401.31542165E-518.94373718
GCCTTAG456.548562E-414.8370191
GGCGAGG1750.012.9899919
ATAGGGG550.002965726412.1431813
GTATTAG550.002972692512.13937951
CCCTTAC550.002972692512.13937951
GTTATAA550.002972692512.13937951
TGTAGGA5150.012.0458992
TGGCGAG4050.011.92753718
GTAGGAC4750.011.8510153
GACGTGG2700.011.6104137
TAGCCCT1251.7109414E-811.4468964
GGCGAGA2400.011.44517319
TATGGCG4400.011.40929516
CTGTAGG5150.011.2975361
ATGGCGA4450.011.28110117
TAGGACC1701.0913936E-1111.2224474
TTGATAC1105.1335155E-711.19402516
TAAGACC600.005689070611.1289254
TATTCTG951.2943872E-511.045255
Produced by FastQC (version 0.11.5)