FastQCFastQC Report
Thu 26 May 2016
SRR1926579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686496
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAA350.005967618419.031111
TTAGACA300.00894355517.7430274
GTTCTAA500.00245224115.9861331
GTGTAGA1003.1250238E-815.9861331
GTCCTAT801.5893065E-514.9869991
GTATAAC550.00462531814.5328471
ATCAGGC450.00473965514.4804533
GTCCTAC2950.014.4507421
GACCTAC658.443387E-414.346531
GTATTAA1059.444302E-713.9561481
TAGGACC1550.013.7365374
CTCTGAA903.9679537E-513.6010732
TCCTACA3350.013.3980742
GGGTATA700.001477758913.3217771
GTATAAG700.001477758913.3217771
GTAGGAC3200.013.2360383
TTAATGC500.00952084813.0324063
TTATACT757.15136E-512.7749814
CAGGCCC757.15136E-512.7749814
GATATAC1152.626426E-612.742571
Produced by FastQC (version 0.11.5)