FastQCFastQC Report
Thu 26 May 2016
SRR1926579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686496
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11530.16795436535682656No Hit
CTGTAGGACGTGGAATATGGCAAGA8160.11886449447629703No Hit
GTCCTACAGTGGACATTTCTAAATT7620.1109984617536009No Hit
GGTATCAACGCAGAGTACTTTTTTT7410.10793944902810797No Hit
GTCCTACAGTGTGCATTTCTCATTT7110.10356943084883234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGG250.006092833418.9652310
TCCAACG551.1541422E-515.50570418
TAGGACC2350.012.9853794
TTCTATA751.4181356E-512.7157832
TGTAGGA5300.012.4158832
TTGCACT550.00297946512.1369044
GACGACT550.00310947712.06790311
CTGTAGG5650.011.977321
GTAGGAC5100.011.7799363
CCTCTAA657.7955564E-411.7307661
CTTCTAT907.1761224E-611.64930251
GGACGTG3450.011.6083626
CAGGACT1001.8382561E-611.4433664
CTCTAGG1354.467438E-911.2962941
GTCCTAC4550.011.1023321
GTCTTAG951.3084829E-511.0361811
TAGGACG3550.011.0135694
GTATAAT700.001449291410.8928541
TTAGGAC2200.010.8365213
TTTAGGA2650.010.796422
Produced by FastQC (version 0.11.5)